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| Tumor associated macrophages(TAMs) are tightly related to carcinogenesis, metastasis and invasion. It has been reported that the phenotype of tumor associated macrophages was rather different from the classical activated macrophages namely M1. TAMs produce anti-inflammtory and immune inhibitory cytokines and chemokines, thus inhibit the anti-tumor immunity within solid tumor. The mechanisms by which the tumor cell polarized macrophages to a pro-tumor state are largely unknown. In this study, we used flow cytometry and ELISA methods to analyze the effect of the condition medium from colon cancer cell line Caoco2 on the THP-1 macrophages. The results showed that the level of M2 markers, CD206 and CD163, were strongly upregulated, the M1 marker CCR7 was significantly downregulated. The cytokines IL-1b, TNF-a, IL-10 were all upregulated. Our research indicated that the tumor supernatant has the ability to induce macrophages to a M2 like phenotype. |
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| Keywords:Tumor associated macrophages; Colon cancer; Cytokines; Macrophages polarization |
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