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| Prostate cancer is a common multiple malignant tumor of male, whose mortality is the 2nd highest of all kinds of tumours in western countries, and the mortality of morbidity is 13% in the USA. The objective of this study was performed to investigate the anti-cancer effect of docetaxel induces apoptosis of prostate cancer through cofilin-1 and paxillin signal path. Treatment docetaxel (1-50 nM) disposed the human prostate cancer (LNCaP) cells for 24 h. Then cell growth and cytotoxicity were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5 -diphenyltetrazolium bromide assay (MTT) and Lactate dehydrogenase (LDH) assay, respectively. Docetaxel-induced cell death was analyzed using Flow cytometric and Caspase-3 assay, respectively. Real-time RT-PCR analysis was used to detect the expression of cofilin-1 gene and western blots was also used to probe the protein expression of paxillin. We found that supplement with docetaxel could inhibit cell growth, promoted cytotoxicity, activated apoptosis and increased caspase-3 activity in LNCaP cell. Interesting, administrate of docetaxel reduced the expression of cofilin-1 gene and the protein expression of paxillin in LNCaP cell. Meanwhile, knockdown of cofilin-1 advanced the anti-cancer effect of docetaxel against LNCaP cell through suppression of paxillin pathway. The present findings that the anti-cancer effect of docetaxel induces apoptosis of prostate cancer through suppression of cofilin-1 and paxillin signal path, and which will help to set a stage to the clinical treatment of prostate cancer. |
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| Keywords:docetaxel, prostate cancer, cofilin-1, paxillin |
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