FYCHWQDE-based affinity chromatography of IgG: Effect of ligand density and purification from human serum

XUE Aiying; YU Linling; SUN Yan

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FYCHWQDE-based affinity chromatography of IgG: Effect of ligand density and purification from human serum

XUE Aiying,YU Linling * #,SUN Yan

Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072

*Correspondence author

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Funding: 高等学校博士学科点专项科研基金（No.No.20130032110028）

Opened online: 4 May 2017

Accepted by: none

Citation: XUE Aiying,YU Linling,SUN Yan.FYCHWQDE-based affinity chromatography of IgG: Effect of ligand density and purification from human serum[OL]. [ 4 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4728711

In our previous work, it has reported on the identification of the octapeptide ligand FYCHWQDE for human IgG (hIgG) affinity chromatography. In this work, effect of ligand density on dynamic binding capacity (DBC) of hIgG on the FYCHWQDE-based resins with ligand densities of 31.1-59.4 μmol/mL and purification of hIgG from human serum were studied. DBC at 10% breakthrough, measured by using 10-fold diluted human serum as feedstock, reached the maximum (27.6±1.3 mg/mL) at 45.3 μmol/mL drained gel and decreased remarkably with increasing flow rate. By using this affinity column with ligand density of 45.3 μmol/mL, hIgG purification from human serum was achieved with high purity (>90%) and recovery yield (82%). The experimental results indicate that the FYCHWQDE-based resin would be a favorable affinity resin for hIgG purification from human serum.

Keywords:Affinity chromatography; Ligand density; Human immunoglobulin G; Dynamic binding capacity; Purification

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