Suppressing Phosphatidylcholine-specific Phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells

Wang Nan; Xie Kun; Huo Siwei; Zhao Jing; Zhang Shangli; Miao Junying

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Suppressing Phosphatidylcholine-specific Phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells

Wang Nan 1 *,Xie Kun 1,Huo Siwei 2,Zhao Jing 1,Zhang Shangli 1,Miao Junying 1

1.Institute of Developmental Biology, School of Life Science, Shandong University

2.Institute of Developmental Biology- School of Life Science- Shandong University

*Correspondence author

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Funding: 教育部博士点基金（No.20050422013）

Opened online:18 October 2006

Accepted by: none

Citation: Wang Nan ,Xie Kun ,Huo Siwei .Suppressing Phosphatidylcholine-specific Phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells[OL]. [18 October 2006] http://en.paper.edu.cn/en_releasepaper/content/8811

In the previous research, we found that D609 (tricyclodecan-9-yl-xanthogenate) could induce human marrow stromal cell (hMSC) differentiation to neuron-like cells. In this study, to understand the possible mechanism, we sequentially investigated the changes of phosphatidylcholine-pecific phospholipase C (PC-PLC) activity, the expression of Rb, the intracellular reactive oxygen species (ROS) levels, NADPH oxidase and superoxide dismutase (SOD) activities when D609 induced neuronal differentiation in rat mesenchymal stem cells (MSCs). The results showed that D609 obviously inhibited the activity of PC-PLC when it induced neuronal differentiation in rat MSCs. Simultaneously, ROS level and the activity of NADPH oxidase increased significantly, but the MnSOD and Cu/ZnSOD activities were not altered. Furthermore, the level of Rb protein was evidently elevated. Our data suggested that PC-PLC mediated neuronal differentiation of rat MSCs by elevating NADPH oxidase activity, ROS level, and up-regulating the expression of Rb protein.

Keywords:mesenchymal stem cells; PC-PLC; neuronal differentiation; ROS

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