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Molecular cloning, characterization and distribution of two types of growth hormone receptor in orange-spotted grouper (Epinephelus coioides)
Li Yun 1 #,Liu Xiaochun 1,Zhang Yong 2,Zhu Pei 2,Lin Haoran 2
1.State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University
2.Laboratory of Biocontrol- Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals- Sun Yat-Sen University
*Correspondence author
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Funding: none
Opened online:23 October 2006
Accepted by: none
Citation: Li Yun,Liu Xiaochun,Zhang Yong .Molecular cloning, characterization and distribution of two types of growth hormone receptor in orange-spotted grouper (Epinephelus coioides) [OL]. [23 October 2006] http://en.paper.edu.cn/en_releasepaper/content/8902
 
 
The cDNAs encoding two distinct growth hormone receptors were firstly cloned and sequenced from the liver of orange-spotted grouper (Epinephelus coioides). The cDNA of grouper GHR1 consisted of 2673-bp and encoded 658 amino acids and that of grouper GHR2 consisted of 2989-bp and encoded 577 amino acids. The two cDNAs had 78.6% identity in nucleotide sequence and 37.8% identity in deduced amino acid sequence. Northern blot analyses demonstrated a single GHR1 transcript of approximately 4.3Kb in liver and a single GHR2 transcript of approximately 3.9Kb in the liver and muscle. In the Real-time PCR assay, grouper GHR1 and GHR2 were expressed in all tissues tested, and the expression of GHR2 was significantly higher than GHR1 in telencephalon, cerebellum, pituitary, heart and white muscle, whereas the expression of GHR1 was significantly higher in liver. The grouper GHR1 had high homology to somatolactin receptor (SLR) and the grouper GHR2 might play a role of modulator of grouper GHR1. These results indicated that there were two types of GHR existing in orange-spotted grouper and they had different structural features and physiological functions. However the general functional differences between GHR1 and GHR2 of orange-spotted grouper remained further research.
Keywords:Growth hormone receptor; tissues expression; orange-spotted grouper
 
 
 

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