Spectrophotometric Determination of Albumin in Serum Using a Novel Near-Infrared Cyanine Dye, 5,5’-Dicarboxy-1,1’-Disulfobutyl-3,3,3’,3’- Tetramethylindotricarbocyanine

Li Weirong; Guo Xiaofeng; Zhang Huashan; Wang Hong

Chinese︱Feedback︱Save this page

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers

Spectrophotometric Determination of Albumin in Serum Using a Novel Near-Infrared Cyanine Dye, 5,5’-Dicarboxy-1,1’-Disulfobutyl-3,3,3’,3’- Tetramethylindotricarbocyanine

Li Weirong 1,Guo Xiaofeng 2,Zhang Huashan 3,Wang Hong 3 #

1.Shenzhen Middle School

2.Department of Chemistry, Wuhan University

3.Department of Chemistry- Wuhan University

*Correspondence author

#Submitted by

Subject:

Funding: 国家自然科学基金（No.20575047）

Opened online:21 November 2006

Accepted by: none

Citation: Li Weirong,Guo Xiaofeng,Zhang Huashan.Spectrophotometric Determination of Albumin in Serum Using a Novel Near-Infrared Cyanine Dye, 5,5’-Dicarboxy-1,1’-Disulfobutyl-3,3,3’,3’- Tetramethylindotricarbocyanine[OL]. [21 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9742

Near-infrared (NIR) dyes (λem > 650 nm) are very promising for the analysis of biological samples, because their long wavelengths allow for minimization of interferences from biomolecule matrices. In this paper, a novel NIR dye, 5,5’-dicarboxy-1,1’-disulfobutyl-3,3,3’,3’-tetramethylindotricarbocyanine (DCDSTCY) has been developed for the spectrophotometric assay of total proteins in serum. Under acidic conditions, the binding of DCDSTCY to proteins caused a new peak at 878 nm, the height of which was proportionable to the concentration of proteins. The linear range was found to be 0.04-0.5 μg mL-1 for bovine serum albumin (BSA) and human serum albumin (HAS) with the detection limits of 5 ng mL-1, respectively. The maximum binding number of BSA with DCDSTCY was measured as 133. The proposed method has been applied to the quantitation of total proteins in serum with recoveries of 96.6-104%.

Keywords:Near infrared (NIR) dye; 5,5’-dicarboxy-1,1’-disulfobutyl-3,3,3’,3’- tetramethylindotricarbocyanine (DCDSTCY); Spectrophotometry; bovine serum albumin (BSA); human serum albumin (HAS); Serum

For this paper

● PDF (0B)
● Revision 0 　　
● Print this paper
● Recommend this paper to a friend
● Add to my favorite list

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded	2045
Bookmarked	0
Recommend	5
Comments	Array

Submit your papers

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item

Saved Papers