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March 15,2014
Volume 7,Issue 5
Pages -
Subject Area:Community Ecology,Ecology,Genetics,Biophysics,Cell Biology,Botany

Title: Community structure of the intertidal macrobenthic fauna in Qushan island of Zhejiang province

Authors: ZHU Sixi, WANG Fengyou, YANG Xiuqing, SU Chunhua, WU Yunjie
PP: 506-511
Abstract: The community structure of the intertidal macrobenthic fauna in Qushan island of Zhejiang province, such as species composition, quantity distribution and biodiversity were analyzed in this paper. The results indicated that the specimen of marcobenthic fauna in this island had 22 species, including 10 species of Mollusca, 10 species of Arthropoda, 1 species of Annelida and 1 species of Chordata. The average biomass and density in all stations were 3 829.25 g/m2 and 4 788 ind/m2, respectively. The Mollusca were the first in biomass and density among all macrobenthic fauna. The value of Shannon-Weaner index, Margalef’s species richness index and Pielou’s evenness index were 0.26-1.22, 0.55-2.12 and 0.12-0.49, and their averages were 0.189±0.435, 1.501±0.707 and 0.023±0.152, respectively. For all of the sections, Cerithidea eingulata was the dominant species, which indicated that the community structure of the intertidal macrobenthic fauna in Qushan island was not stable and the biodiversity was also lower. The main factors of this result were human activities, such as excessive exploitation, habitat fragmentation.
Keywords: community ecology; Qushan island; marcobenthic fauna; community structure; intertidal zone

Title: Analysis on the spider diversity in the northeastern scenic areas in Jiangxi province

Authors: CNEN Lianshui, YUAN Fenghui, RAO Jun, ZHOU Guchun, TANG Xing
PP: 497-505
Abstract: The survy on spider resources and fauna of Longhushan scenic area in Yingtan city（A）, Guifeng scenic area in Yiyang county（B）, Shangrao camp scenic spot（C）, the southern suburbs of Yushan county（D） and Sanqingshan scenic area in Yushan county（E）, which are located at northeastern in Jiangxi province, obtain 708 copies of spider specimens. By the classification and identification firstly, they are attached to 18 families, 54 generas and 95 species, of which 6 species are new recorded in Jiangxi province and 9 species are undetermined. Spider diversity studies have shown that the size of the richness index M order is D>C>E>B>A, dominance index H′ order is E>A>C>B>D, diversity index D order is B>A>C>D>E, the size of the evenness index J as follows: D>B>C>A>E. Finally, spider communities between the cluster analysis show that the spider community between Longhushan scenic area in Yingtan city （A） and Guifeng scenic area in Yiyang county （B） is the more similar and continuous, with frequent exchanges of spider species, then the continuity degree between the other spider communities is followed by Shangrao camp scenic spot （C）, the southern suburbs of Yushan county （D） and Sanqingshan scenic area in Yushan county （E）, the degree of spider species in exchange successive reduce.
Keywords: community ecology; northeastern in Jiangxi province; scenic area; spider; diversity

Title: Fragmentation process of wetlands landscape and its driving forces analysis in the middle reaches of the Heihe river around water redistribution

Authors: JIANG Penghui, ZHAO Ruifeng
PP: 487-496
Abstract: In this paper, the wetland landscape fragmentation process of the middle Heihe river basin around water redistribution was analyzed by choosing some typical landscape index models, such as patch density (PD), mean patch size (MPS), mean patch shape fragmentation index (FS1) and area-weighted mean patch fragmentation index (FS2) based on satellite images of 1992, 2001 and 2010. Furthermore, the driving force of wetland landscape fragmentation process was researched through combining with Pearson correlation analysis, regression analysis and other analysis methods. The results indicated that fragmentation process of wetland landscape in the middle reaches of the Heihe river mainly expressed as the shrinkage of MPS, the increase of PD and the enlargement of FS. In the entire period of study, the MPS of the wetlands in middle Heihe river basin decreased 1.47 hm2 corresponding to the PD raised 0.002 ind/hm2. The dominant driving force which led to the middle reach of the Heihe river wetland landscape fragmentation and development included nature and human factors. The natural factors such as hydrology, temperature and precipitation had significantly influence on the process of wetland landscape fragmentation, and the temperature played a greater actor in the process of wetland landscape fragmentation compared with precipitation. The human factors mainly included population and industrial and agricultural production.
Keywords: ecology; middle reach of the Heihe river; fragmentation process; driving forces

Title: Research of chromosome specific molecular markers for Thinopyrum elongatum in a wheat background

Authors: CHEN Shiqiang, ZHU Xue, ZHANG Chao, CAO Wenguang, GEORGE Fedak, CHEN Xiulan, CHEN Jianmin
PP: 476-486
Abstract: 11 primers were synthesized according to the reverse transcriptase and long terminal repeated (LTR) conserved regions of retrotransposons BARE-1 from barley and RIRE-1 from rice. 52 pairs of primer combinations based on these 11 primers were used for DNA amplification of Chinese spring (CS)-Thinopyrum elongatum addition lines, substitution lines plus the two parents. The results showed that 145 specific framents of inter-simple sequence repeat (ISSR), inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon microsatellite amplified polymorphism (REMAP) were obtained which distributed over all the 7E genome chromosomes of Th. elongatum. 60 specific fragments of ISSR, IRAP and REMAP were randomly selected for cloning and sequencing. 34 sequences were found not to be homologous with wheat sequences, which were considered to be the specific sequences of Th. elongatum. Thirty-four primers acording to the 34 specific sequences were synthesized, and 12 chromosome-specific markers of Th. elongatum were obtained which were furtherly converted into sequence-characterized amplified regions (SCAR) markers. The results indicated that ISSR, IRAP and REMAP techniques can be used to develop chromosome-specific SCAR markers with good stability and repeatability. These specific SCAR markers can now be used to detect and identify Th.elongatum chromosomes in a wheat background and possibly even some introgressed segments.
Keywords: genetics; Thinopyrum elongatum; chromosome-specific molecular markers; sequence characterized amplified region

Title: Research on the transport mechanism of the fucose transporter FucP using accelerated algorithm

Authors: LIU Yufeng, SUN Linfeng, GONG Haipeng
PP: 469-475
Abstract: In this paper, we employ an accelerated algorithm to simulate L-fucose/proton symporter (FucP) to reveal the transport mechanism of major facilitator superfamily (MFS). Preliminary results indicate that there is an occluded conformation existed in FucP besides the canonical outward-open and inward-open conformations. The transitions between these conformations do not follow the rigid body movement exactly. Instead, there are considerable structural rearrangements within domains, especially for the N domain. In addition, the simulations identify the indispensable role of the 135 residues to glutamic acid (E135) in both substrate transport and conformational change, and outline the influence of the 312 residues to arginine (R312), the 159 residues to glutamine (Q159) and the 365 residues to tyrosine (Y365) on the structural transition. Finally, we propose a possible mechanism to elucidate the coupling between substrate delivering and conformational change in FucP.
Keywords: biophysics; major facilitator superfamily; self-guided Langevin dynamics; L-fucose/proton symporter; molecular dynamics simulation

Title: ISSR polymorphism analysis on BC1 progenies between Saccharum officenarum and Erianthus fulvus

Authors: YUAN Shenhong, LI Fusheng
PP: 462-468
Abstract: The Saccharum officenarum (sugarcane) Yacheng 89-9 was hybridized with wild Erianthus fulvus to produce the F1 generation. Since the genetic of sugarcane had great variability, the F1 generation was backcrossed with Xintaitang 10 and Yacheng 93-26 respectively. Inter-simple sequence repeat (ISSR) markers analysis on BC1 progenies that derived from intergeneric hybridization and their parents was performed. The results showed that totally 121 bands were amplified using 11 primers, among which 109 were polymorphic bands and the polymorphism was 90.08%, indicating that there were more broad genetic differentiation among the BC1 progenies. Clustering result showed that BC1 progenies were preferential cluster with female parents, indicating that genetic material of female parents were predominant in BC1 progenies.
Keywords: molecular biology; Saccharum officenarum; Erianthus fulvus; inter-simple sequence repeat marker; polymorphism

Title: Functional research on PAM gene of taxol-producing fungus NK-101

Authors: YAN Fei, WANG Yu, LI Qiang, MA Tianzhu, ZHU Xudong, PAN Jiao
PP: 453-461
Abstract: We have isolated a taxol-producing fungus—Pestalotiopsis malicola named NK-101, and finished the whole genome sequencing and annotating work of NK-101. Comparing with the amino acid sequences of phenylalanine aminomutase (GenBank: AAT47186.1) in Taxus canadensis, we have found the homologous gene in NK-101 and named it PAM gene. Based on the homologous recombination and agrobacterium-mediated transformation, we constructed PAM gene deletion strain, named Δpam. Comparing to NK-101, the growth rate, asexual spore production capacity, pigment secretion capacity, mycelial biomass and secondary metabolites synthesis capacity have decreased in Δpam. Based on the above factors mentioned, we demonstrated that the PAM gene is closely related to the growth, asexual spore production, pigment secretion and secondary metabolites in NK-101.
Keywords: molecular biology; Pestalotiopsis malicola; PAM gene; agrobacterium-mediated transformation; gene knockout

Title: Prediction of genes regulated by JAK-STAT signal pathway of Bombyx mori

Authors: LIU Jiabin, QIAN Ying, XUE Renyu, CAO Guangli, GONG Chengliang
PP: 445-452
Abstract: In the present study, the genes regulated by Janus kinase/signal transducer and activator of transcription pathway (JAK-STAT) signal pathway were predicted by bioinformatics analysis. 1 000 bp of upstream promoter sequence of the all predicted genes were downloaded from the silkworm genome database, and the STAT binding sequence TTCN2-4GAA was searched by scanning the promoter sequences. The results showed that 1-6 the target sequences could be found in the upstream promoter sequences of 9 293 genes coding 7 271 non-redundant proteins. Gene ontology (GO) annotation results showed that these proteins were involved in cellular component, molecular function and biological process, suggesting JAK-STAT pathway played an important role in many ways. More than 50% genes were related to binding, about 40% genes were related to cellular process, metabolic process and catalytic activity in the targeting genes. It is considered that JAK-STAT plays a role in immune response, 1-4 STAT binding sequences could be detected in promoter region of some genes related to anti-viral factors, cellular immune effector, and small antimicrobial peptide such as defensin, attacin, moricin and gloverin3, implying that the expression of some anti-viral factors, cellular immune effectors and antimicrobial peptides related to antiviral activity might be regulated by JAK-STAT signal pathway.
Keywords: molecular biology; Janus kinase/signal transducer and activator of transcription pathway; Bombyx mori; gene ontology annotation; immune response

Title: Identification of miRNA-34c expression in mouse testis and spermatogonial stem cells by FISH

Authors: YU Meng, LIU Weishuai, HUA Jinlian
PP: 440-444
Abstract: Objective: To explore the expression of miRNA-34c (miR-34c) in mouse testis tissues and mouse spermatogonial stem cells (SSCs). Methods: The technology of fluorescence in situ hybridization (FISH) was used to illustrate the miR-34c expression in mouse testis tissues and SSCs. Results: The expression of miR-34c was higher in 2 month old mouse testis tissue slices. Furthermore, the expression of miR-34c was higher in the group of miR-34c-transfected mimics. Conclusion: FISH is a stable and repeatable method in measuring miRNA expression.
Keywords: cell biology; fluorescence in situ hybridization; spermatogonial stem cell; testis; mouse

Title: Establishment of transgenic Kunming white mice expressing green fluorescence protein

Authors: YANG Baotian, LIU Tao, ZHAO Wuyang, QIAN Qijun
PP: 435-439
Abstract: Objective: To establish transgenic Kunming white mice expressing green fluorescence protein (GFP), provide outbred animal models carrying reporter gene for related studies. Methods: Using infected Kunming mouse embryonic stem cell line KES with a lentivirus carrying GFP as a reporter gene, an embryonic stem cell (ESC) line KES-GFP expressing GFP was generated efficiently and stably. These KES-GFP cells were introduced into host embryos from 129 stain mouse through aggregation to form chimeric blastocysts, then transferred it into uterus of pseudopregnant mice to obtain chimeric mice expressing GFP. These chimeric mice were mated with Kunming mice to obtain transgenic mice. Results: We obtained transgenic Kunming white mice expressing GFP successfully. Conclusion: Transgenic Kunming white mice expressing GFP can be successfully established based on ESCs.
Keywords: cell biology; embryonic stem cell; transgenic mouse; green fluorescence protein; chimera

Title: Comparison of haploid strain selection of Saccharomyces cerevisiae and identification methods

Authors: ZHANG Jinjin, HUANG Guangqing, LI Zhibin, LIN Jianghai, GONG Yingxue, LI Jingbo, XIAO Wenjuan, LIU Zehuan
PP: 428-434
Abstract: Objective: To evaluate the five methods of identification haploid strain for molecular biology research. The haploid strains of AS line from the industrial strain AS2.489 of Saccharomyces cerevisiae have been obtained by sporulation. Methods: The five methods including comparison of the morphological characteristic of diploid and haploid, cross breeding, specific PCR, concentration analysis genomic DNA, andstress tolerance of ethanol and glucose osmotic pressure were used and their difference was comparied. Results: With the regard to the stability and accuracy of haploid strain, the cross breeding and specific PCR were more stable than the other three. others were able to be applied to separate haploid strain from diploid strain. Three stable haplotype strains (two MATa, AS-5 and AS-21, and one MATα AS-7) were separated in this study. Conclusion: One of other methods combined with cross breeding may be suitable for selection of haploid strain from yeast diploid.
Keywords: microbiology; Saccharomyces cerevisiae; haploid; selection; identification

Title: Over-expression of AtAGP11 gene leads to abortion in Arabidopsis thaliana

Authors: ZHONG Jing, ZHANG Xuelian, ZHAO Jie
PP: 417-427
Abstract: In this paper, we adopted pollen and embryo separation, pollen DAPI dyeing, artificial pollination, semi-thin section technologies and biostatistics to observe the phenotypes of AtAGP11 over-expression transgenic plants and found serious abortion phenomenon in the AtAGP11 over-expression plants. Further research showed that over-expression of AtAGP11 affected pollination by shortening the length of filaments so that pollen could not land on stigma, which resulted in abortion phenotype. This study discovered that over-expression of AtAGP11 affected regular growth of filaments, indicating a key role of AtAGP11 in cell growth.
Keywords: plant developmental biology; Arabidopsis thaliana; AtAGP11 gene; over-expression; abortion

Title: Effects of exogenous GA3 on seed germination of Cynomorium songaricum Rupr. under NaCl stress

Authors: SONG Zhaowei, CHEN Guilin, LIU Guangda
PP: 408-416
Abstract: To understand the growth character of halophytic medicinal root parasitic plant Cynomorium songaricum Rupr., we used C. songaricum seeds to study the germination and proteomics in order to reveal the role of NaCl and gibberellic acid (GA3) in the establishment of relationship between C. songaricum seed and its host. We added exogenous GA3 in NaCl environment and recovery conditions, determined the proteomics and the percentages of seed germination, recovery germination and final germination under different temperature conditions. The result showed that exogenous GA3 could increase the germination percentage of C. songaricum seed under NaCl environment and recovery conditions, under the conditions of 25℃, 200 μmol/L GA3+100 mmol/L NaCl, the C. songaricum seed reached the highest germination percentage (7.00±1.91)%, the non-germinated seeds were transferred to another petridish under condition of 25℃, 200 μmol/L GA3 for recovery germination, the germination percentage reached the maximum (32.32 ± 2.89)%. After 40 d treatment, the final germination percentage were mainly contributed from recovery germination stage, NaCl environment with GA3 was regarded as the preconditioning stage of seed germination, and it could activate the gibberellin receptor (GID1). The preculture seeds germ tube was longer and could prevent browning, the haustorial was more obvious. Therefore, the exogenous GA3 can induce and promote C. songaricum seed germination under NaCl and the NaCl reduced environment.
Keywords: botany; Cynomorium songaricum Rupr.; seed germination; halophytic medicinal herb; root parasitic plant; proteomics

Title: Study on phylogenetic relationship within Euplotes-complex using RAPD technology

Authors: YI Zhenzhen, ZHANG Yi
PP: 403-407
Abstract: The present work used 19 random primers to explore phylogenetic relationship in 13 species/14 populations of Euplotes-complex, and the consequence of electrophoresis reflected the significant differences in different species clearly.Based on the genetic similarity and neighbor joining (NJ) tree constructed by RAPDistance, the results showed that Euplotes-complex could be separated well among different species, and it had good homologous in different populations at the same time. The results of the genetic distance tree indicated that populations of Euplotoides (Ed.) parawoodruffi group clustered into one branch, while Euplotopsis (Ep.) orientalis, Ep. raikovi and Ep. encysticus did not fall into the same clade with showing a certain genetic distance.
Keywords: zoology; Euplotes-complex; random amplified polymorphic DNA fingerprint; distinction between species; genetic relationship

Title: Research progress of PDTX model

Authors: LIANG Wenfeng, ZHENG Lin, FENG Yuntian, ZHANG Hao
PP: 398-402
Abstract: Patient-derived tumor xenograft (PDTX) is an important model of tumor xenograft for mice. This paper reviewed the classification, establishment, generation of tumor xenograft and impact factors for successful transplanting of PDTX model. At the same time, this paper summaried and prospected the biological characters and the relationship of primary tumors of PDTX model, and the application of PDTX model in tumor research as well. This review provided theory evidence and help for individualized treatment for tumor based on PDTX model which had human tumor characters.
Keywords: zoology; patient-derived tumor xenograft; review; primary tumor; drug trial

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