Optimization of an enzyme production medium and bioconversion conditions for L (+)-tartaric acid production by Corynebaterium sp. in fed-batch culture

Wenpeng Li; Jinlun Xie

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Optimization of an enzyme production medium and bioconversion conditions for L (+)-tartaric acid production by Corynebaterium sp. in fed-batch culture

Wenpeng Li * #,Jinlun Xie

Key Laboratory of Industrial Microbiology & Fermentation Technology of Yunnan, Yunnan University

*Correspondence author

#Submitted by

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Funding: 云南省教委基金项目（No.9642037）

Opened online:24 February 2007

Accepted by: none

Citation: Wenpeng Li,Jinlun Xie.Optimization of an enzyme production medium and bioconversion conditions for L (+)-tartaric acid production by Corynebaterium sp. in fed-batch culture[OL]. [24 February 2007] http://en.paper.edu.cn/en_releasepaper/content/11195

The medium for production of cis-epoxysuccinate hydrolase from Corynebaterium sp. YNUCC0211 and bioconversion conditions for L (+)-tartrate production were optimized by means of statistical methods respectively. Results revealed that the yields of L (+)-tartrate were improved by feeding fresh synthesized cis-expoxysuccinate solution with 12.0 of pH or with 3% of hydrogen peroxide to the optimum medium after being inoculated for 32-48 hours. The optimum medium contains (g/litre): glucose 15, cornsteep liquor 3.0, yeast extraction 0.3, K2HPO4.3H2O 3.5, dipotassium cis-epoxysuccinate 7.0, NH4NO3 4.0, (NH4)2 SO4 1.0, MgSO4.7H2O 1.0, FeSO4.7H2O 0.02, pH 7.0 - 7.2.

Keywords:OADs, Corynebaterium, L (+)-tartaric acid, cis-Expoxysuccinate, Bioconversion

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