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Cloning and expression of a bactericin sakacin P from Lactobacillus sakei in Escherichia coli
Shuo Li,Haiqin Chen ,Fengwei Tian, ,Hao Zhang,Wei Chen * #
School of Food Science and Technology, Jiangnan University
*Correspondence author
#Submitted by
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Funding: Nation Natural Science Foundation ,Ministry of Education,Ministry of Education（No.20706023,SKLF-MB-200802,IRT0627）
Opened online:15 October 2008
Accepted by: none
Citation: Shuo Li,Haiqin Chen ,Fengwei Tian, .Cloning and expression of a bactericin sakacin P from Lactobacillus sakei in Escherichia coli[OL]. [15 October 2008] http://en.paper.edu.cn/en_releasepaper/content/24827
 
 
Sakacin P, a novel bactericin from Lactobacillus sakei, is a potential antimicrobial substrate, which has strong activity against food-borne pathogen eg. Listeria monocytogenes. In Lactobacillus sakei, the gene (sppA) of sakacin P was controled by strict regulation mechnism, so that the quantity of secreted sakacin P was low. In this study, we synthesized the sppA gene using splicing by overlap extension PCR (SOE-PCR), and cloned it into E. coli expression system. After the induction of IPTG, the recombinant sakacin P was successfully expressed. The collected cells were sonicated and the activity was identified by inhibition zone method using the cell-free supernatant. The results also showed that the low temperature introduction took more active sakacin P which has strong antimicrobial activity.
Keywords:sakacin P; expression; class IIa bacteriocins
 
 
 

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