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In this work, the interactions of the single ingredient (Ginsenoside Rb1, Rg1 and Re) and the multi-ingredients (Rb1 plus Rg1, Rb1 plus Re, Rg1 plus Re) with bovine serum albumin (BSA) were investigated by fluorescence spectroscopy. They were found to quench the intrinsic fluorescence of BSA via static quenching and the hydrophobic and electrostatic forces might play a major role in these reactions. With the gradual adding of Rb1, Rg1 or Re to BSA solution, the fluorescence intensity of BSA decreased by 13.3%, 6.5% and 13.0%, respectively. Further studies show no any two-drug combinations exiting synergistic effect or antagonism, thus the binary mixture had an additive effect on BSA quenching. |
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Keywords:Ginsenoside Rb1; Ginsenoside Rg1; Ginsenoside Re; bovine serum albumin; fluorescence; additive effect |
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