Home > Papers

 
 
Synthesis of amino-group functionalized superparamagnetic iron oxide nanoparticles and applications as biomedical labeling probes
MA Ming 1 #,ZHAN Yanqiang 2,SHEN Yaqi 3,XIA Xing 1,ZHANG Suming 4,LIU Zuli 5 *
1.School of Physics, Huazhong University of Science and Technology (HUST), Wuhan 430074, Hubei Province, People\'s Republic of China
2.Department of Neurology, Tongji Hospital, Huazhong University of Science and Technology (HUST), Wuhan 430030, Hubei Province, People\'s Republic of China
3.Department of Radilogy, Tongji Hospital, Huazhong University of Science and Technology (HUST), Wuhan 430030, Hubei Province, People\'s Republic of China
4.Department of Neurology, Tongji Hospital, Huazhong University of Science and Technology (HUST), Wuhan 430030, Hubei Province, People\'s Republic of China
5.School of Physics, Huazhong University of Science and Technology, Wuhan 430074, Hubei Province, People\'s Republic of China
*Correspondence author
#Submitted by
Subject:
Funding: The Research Fund for the Doctoral Program of Higher Education （No.No.20090142110064）
Opened online:25 January 2011
Accepted by: none
Citation: MA Ming,ZHAN Yanqiang,SHEN Yaqi.Synthesis of amino-group functionalized superparamagnetic iron oxide nanoparticles and applications as biomedical labeling probes[OL]. [25 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4407416
 
 
Superparamagnetic iron oxide (SPIO) nanoparticles were synthesized by coprecipitation technique and further functionalized with amino-group to obtain amino-group functionalized (amino-SPIO) nanoparticles. The X-ray diffraction results reveal the structure of amino-SPIO nanoparticles, from which the average iron core diameter is approximately 10 nm by calculation; while Zetasizer reveals their hydrodynamic diameter are mainly distributed in the range of 40-60 nm. These nanoparticles can be taken up by liver tissue, resulting in dramatically darkening of liver tissue under T2-magnetic resonance imaging (MRI). The spin-spin relaxivity coefficient of these nanoparticles is 179.20 mM-1sec-1 in a 1.5 T magnetic resonance system. In addition, amino-SPIO nanoparticles were conjugated to Tat (FITC) peptide and incubated with neural stem cells in vitro, we can detect the positive-labeling (labeled) neural stem cells showing green fluorescence, which indicates Tat (FITC) peptide derivated amino-SPIO nanoparticles are able to enter cells. Furthermore, we also find significant negative T2 contrast enhancement when compared with the non-nanoparticles-labeled neural stem cells in T2-weighted MRI. The amino-SPIO nanoparticles show promising potential as a new type of labeling probes, which can be used in magnetic resonance-enhanced imaging and fluorescence diagnosis.
Keywords:amino-group functionalization; superparamagnetic; MRI; T2 relaxivity; fluorescence labeling
 
 
 

For this paper
  • ● PDF (0B)
  • ● Revision 0 　　
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags
Add yours

Related Papers

Statistics
PDF Downloaded 446
Bookmarked 0
Recommend 5
Comments Array
Submit your papers