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The cathelicidin-like peptide derived from panda genome is a potential antimicrobial peptide
Yan Xiuwen 1 #,Zhong Jian 2,Liu Huan 3,Liu Cunbao 3,Zhang Keyun 4,Lai Ren 4 *
1.Life Sciences College of Nanjing Agricultural University, NanJing 210095
2.Nanjing Maternal and Child Health Hospital No. 123 Tianfeixiang, Nanjing, 210004, China
3.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
4.Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
*Correspondence author
#Submitted by
Subject:
Funding: Chinese National Natural Science Foundation (No.31070701, 31000962, 31025025), the Ministry of Science and Technology (No.2010CB529800, 2009ZX09103-1/091, 2011ZX09102-002-10), the Ministry of Agriculture (No.2009ZX08009-159B)
Opened online:16 February 2012
Accepted by: none
Citation: Yan Xiuwen,Zhong Jian,Liu Huan.The cathelicidin-like peptide derived from panda genome is a potential antimicrobial peptide[OL]. [16 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4463680
 
 
A novel cathelicidin-like antimicrobial peptide was identified by mining genome of panda. This peptide(cathelicidin-AM) was synthesized. It showed potential antimicrobial activities against wide spectrum of microorganisms including Gram-negative and -positive bacteria, and fungi. It had similar antimicrobial abilities against both standard and clinically isolated drug-resistant strains. Cathelicidin-AM could rapidly exert its antibacterial activities. It just took less than 1 h to kill all Staphylococcus sciuri at the concentration of 2, 4 or 10 times of minimal inhibitory concentration (MIC) while clindamycin took 6 h. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analysis indicated that cathelicidin-AM killed bacteria by directly affecting bacterial cell wall and membrane. Phylogenetic analysis revealed that the panda cathelicidin had the nearest evolution relationship with dog cathelicidin. The current work provides a novel cathelicidin-like peptide with strong antimicrobial abilities.
Keywords:cathelicidin; antimicrobial peptide ; panda
 
 
 

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