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Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata
Liu Xiuhong 1,Liu Rui 1 #,Wei Lin 1,Yang Hailong 2,Zhang Keyun 1,Liu Jingze 3,Lai Ren 1 *
1.Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China
2.Key laboratory of Animal models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences
3.College of Life Sciences School of Hebei Normal University
*Correspondence author
#Submitted by
Subject:
Funding: Chinese Academy of Sciences (No.KSCX2-YW-G-024), the Ministry of Science and Technology)
Opened online: 9 March 2012
Accepted by: none
Citation: Liu Xiuhong,Liu Rui,Wei Lin.Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata[OL]. [ 9 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4465356
 
 
Two novel antimicrobial peptides with similarity to brevinin-2 family are purified and characterized from the skin secretions of the frog, Rana nigrovittata. Their amino acid sequences were determined as GAFGNFLKGVAKKAGLKILSIAQCKLSGTC (brevinin-2-RN1) and GAFGNFLKGVAKKAGLKILSIAQCKLFGTC (brevinin-2-RN2), respectively, by Edman degradation. Different from brevinin-2,which is composed of 33 amino acid residues (aa), both brevinin-2-RN1 and -RN2 contain 30 aa. Five cDNA sequences (Genbank accession numbers, EU136465-9) encoding precursors of brevinin-2-RN1 and -RN2 were screened from the skin cDNA library of R. nigrovittata. These precursors are composed of 72 aa including a predicted signal peptide, an acidic spacer peptide, and a mature brevinin-2-RN. Both brevinin-2-RN1 and -RN2 showed strong antimicrobial activities against gram-positive and gram-negative bacteria and fungi. The current work identified and characterized two novel antimicrobial peptides with unique primary structure.
Keywords:antimicrobial peptides; amphibian; skin; brevinin-2; Rana nigrovittata
 
 
 

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