Home > Papers

 
 
Introducing human Kringle 1-5 gene into MSC blocks angiogenesis
LIU Haixia 1 #,CHU Yinzhu 2,LOU Ge 3 *
1.The Third Affiliated Hospital of Harbin Medical University, Harbin 150081
2.The First Affiliated Hospital of Harbin Medical University
3.The Third Affiliated Hospital of Harbin Medical University
*Correspondence author
#Submitted by
Subject:
Funding: Programs Foundation of the Department of Education of Heilongjiang Province (No.No. 12511330), Specialized Research Fund for the Doctoral Program of Higher Education (No.No. 20092307110018), The Key Program of the Heilongjiang Provincial Science and Technology Committee (No.No. GC10C302), Programs Foundation of the Department of Health Heilongjiang Province(No.No.2012661), Programs Foundation of the Affiliated Tumor Hospital of Harbin Medical University (No.No. JJZ2011-06), Programs Foundation of Natural Science of the Heilongjiang (No.No. 41400453-8-12132/ H1621)
Opened online:26 November 2012
Accepted by: none
Citation: LIU Haixia,CHU Yinzhu,LOU Ge.Introducing human Kringle 1-5 gene into MSC blocks angiogenesis[OL]. [26 November 2012] http://en.paper.edu.cn/en_releasepaper/content/4494254
 
 
In this paper, Mesenchymal stem cell (MSC), as a cell tool, can be utilized to deliver anti-cancer molecules locally. However, MSC release some pro-angiogenic factors and may promote stem cell-mediated tumor angiogenesis. Here, we efficiently engineered human placenta MSC (HPMSC) to deliver kringle 1-5 protein (K1-5), an angiogenesis inhibitior, and conditioned medium from the expressed K1-5 protein of HPMSCS (K1-5-CM) could reduce VEGF-induced proliferation and tube formation of human umbilical veins endothelial cell (HUVEC). Moreover, Administration of K1-5-CM blocked STAT3 activation of HUVEC with VEGF stimulation and introducing K1-5 gene into HPMSC inhibited STAT3 activation and VEGF release induced by TNF-a. Take together, these data demonstrate that MSC, as cell tool of gene therapy ,engineered to express anti-angiogenesis gene not only block angiogenesis of endothelial cell in vitro but also reverse the role of MSC mediated angiogenesis and it may be a safe therapeutic option by suppressing angiogenesis in tumor.
Keywords:Gene therapy; Mesenchymal stem cell; Kringle 1-5; Angiogenesis; Endothelial cell
 
 
 

For this paper

  • PDF (0B)
  • ● Revision 0   
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded 196
Bookmarked 0
Recommend 5
Comments Array
Submit your papers