Determination of 5-Hydroxyindole-3-acetic acid, dihydroxyphenylacetic acid and homovanillic acid in the brains of freely moving rats using microdialysis coupled with HPLC-ECD

LIU Rui; DUAN Jin-ao; GUO Jianming; TANG Yuping; QIAN Dawei

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Determination of 5-Hydroxyindole-3-acetic acid, dihydroxyphenylacetic acid and homovanillic acid in the brains of freely moving rats using microdialysis coupled with HPLC-ECD

LIU Rui 1 #,DUAN Jin-ao 2 *,GUO Jianming 2,TANG Yuping 2,QIAN Dawei 2

1.College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046

2.Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046

*Correspondence author

#Submitted by

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Funding: none

Opened online:18 January 2013

Accepted by: none

Citation: LIU Rui,DUAN Jin-ao,GUO Jianming.Determination of 5-Hydroxyindole-3-acetic acid, dihydroxyphenylacetic acid and homovanillic acid in the brains of freely moving rats using microdialysis coupled with HPLC-ECD[OL]. [18 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4511624

In present paper, microdialysis (MD) combined with high performance liquid chromatography with electrochemical detection (HPLC-ECD) was applied for the determination of 5-hydroxyindole-3-acetic acid (5-HIAA), dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the hypothalamus of LPS-induced hyperthermia rats. The extracellular neurotransmitters of 5-HIAA, DOPAC and HVA could be detected increasing significantly in rat brain 150 min after LPS injection. The result showed that dynamic change of neurotransmitters in rat brain could be determined easily by MD coupled with HPLC-ECD. Furthermore, the advantage that fewer animals sacrifice occurred in the microdialysis experiment was confirmed.

Keywords:Microdialysis; HPLC-ECD; Neurotransmitters; Hyperthermia

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