Home > Papers

 
 
Enhancing fatty acid production in E.coli by overexpressing the reducing power generating enzymes
Zhou Yongshuang 1 #,Zhang Huaiyuan 2,Chen Haiqin 2,Song Yuanda 2 *,Chen Yongquan 2,Zhang Hao 2,Chen Wei 2
1.School of Food Science and technology, Jiangnan University, JiangSu WuXi 214122
2.School of Food Science and technology, Jiangnan University, WuXi 214122
*Correspondence author
#Submitted by
Subject:
Funding: the National High Technology Research and Development Program of China(No.No. 2012AA022105C), the National Science Fund for Distinguished Young Scholars (No.No. 31125021), the National Natural Science Foundation of China(No.No. 31271812)
Opened online:20 March 2013
Accepted by: none
Citation: Zhou Yongshuang,Zhang Huaiyuan,Chen Haiqin.Enhancing fatty acid production in E.coli by overexpressing the reducing power generating enzymes[OL]. [20 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4529473
 
 
Reducing power is an essential factor for fatty acid biosynthesis. In this study, we investigated the effect of five reducing power generating enzymes (NAD-dependent malic enzyme (NAD-ME), NADP-dependent malic enzyme (NADP-ME), NADP-dependent isocitrate dehydrogenase (NADP-IDH), glucose 6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (PGD)) on fatty acid biosynthesis in an engineered E. coli BL21 △fadE/pTE which has a fatty acid metabolic sink. Overexpression of NAD-ME, NADP-IDH and G6PD resulted in 2.15-, 1.6- and 1.16-fold increase in fatty acid yield respectively, while overexpression of PGD didn't lead to a significant increase of fatty acid production and overexpression of NADP-ME led to a 15% decline in fatty acid yield. This study provides a feasible strategy for enhancing fatty acid production in engineered E. coli strain by overexpressing the reducing power generating enzymes NAD-ME and G6PD.
Keywords:Fatty acid biosynthesis; Reducing power; Overexpression
 
 
 

For this paper

  • PDF (0B)
  • ● Revision 0   
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded 389
Bookmarked 0
Recommend 6
Comments Array
Submit your papers