The protective effect of organic media on protein conformation of covalent immobilization enzyme

SU Jianhua; CHU Maoqing; ZHU Xiongjun; LIU Daling

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The protective effect of organic media on protein conformation of covalent immobilization enzyme

SU Jianhua 1,CHU Maoqing 2,ZHU Xiongjun 2,LIU Daling 2 *

1.Institute of Microbial Technology, Jinan University, GuangZhou 510632

2.Institute of Microbial Technology,Jinan University,Guangzhou 510632

*Correspondence author

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Funding: Guangdong Province Rural Science and technology key project （No.2012A020200003）, The national key basic research and development plan（No.2013CB127804）

Opened online:12 April 2013

Accepted by: none

Citation: SU Jianhua,CHU Maoqing,ZHU Xiongjun.The protective effect of organic media on protein conformation of covalent immobilization enzyme[OL]. [12 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4535583

In order to know the feasibility of protecting active conformation of enzyme during the covalent-immobilization, three lyophilized enzymes catalase (CAT), trypsin (TRY) and laccase (LAC) are immobilized under organic media and aqueous media, and kinetic constants are investigated. Specific activity of CAT immobilized in chloroform is 1.67 times larger with Kcat 2.43 times higher and Kmapp is 0.74 times smaller than that of CAT immobilized in aqueous phase. Specific activity of TRY immobilized in ethyl acetate is 5.33 times larger with Kcat 55.58 times higher and Kmapp is 0.40 times smaller than that of TRY immobilized in aqueous phase. Specific activity of LAC immobilized in ethyl acetate is 0.24 times larger with Kcat 0.29 times higher and Kmapp is 0.27 times smaller than that of LAC immobilized in aqueous phase.Results show that organic media has more advantages over aqueous media to remarkably improve activities and kinetic constant values. Organic media makes the enzymes under〝rigid〞state, and this "rigid" state helps enzymes maintain their original active conformation and prevent enzymes from conformation disorder during the modification reaction occurring.

Keywords:Enzyme; Covalent immobilization; Conformation; Organic media

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