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To comprehend the gene expression profile in rice panicle under high temperature, Agilent 4×44K rice oligo microarray experiments were carried out using post-meiosis stage rice panicle treated at 40 centigrade degree for 0 min, 10 min, 20 min, 60 min, and 2 hr. The time course differentially expressed genes under heat stress were mainly involved in binding, catalysis, stress response, and cellular process. The significantly changed genes during heat treatment were mainly up-regulated. Among heat-responsive genes, the predominant transcription factor gene families were Hsf, NAC, AP2/ERF, WRKY, MYB, and C2H2. The MapMan analysis demonstrated that, under heat treatment, the HR genes were enriched in the pathways related to biotic and abiotic stress, cell cycle, development, ubiquitin-proteasome system, lipid and secondary metabolisms. These data revealed the great importance of cross-talk and protein homeostasis in response to heat stress in rice panicle at post-meiosis stage. |
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Keywords:Rice; Young panicle; Microarray; Heat stress |
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