Electrochemiluminescent immunosensor based on  magnetic nanofibers/carbon nanohorns for ultrasensitive detection of aflatoxin B1

Wang Yu; Xu Guifang; Zhang Qingrong; Yan Huixiang; Zhang Shupei; Li Yilin; Gong Lingshan; Dai Hong

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Electrochemiluminescent immunosensor based on magnetic nanofibers/carbon nanohorns for ultrasensitive detection of aflatoxin B1

Wang Yu #,Xu Guifang,Zhang Qingrong,Yan Huixiang,Zhang Shupei,Li Yilin,Gong Lingshan,Dai Hong *

College of Chemistry and Chemical Engineering, Fujian Normal University, Fujian 350007

*Correspondence author

#Submitted by

Subject:

Funding: This project was financially supported by NSFC （No.21205016), National Science Foundation of Fujian Province）

Opened online:10 September 2015

Accepted by: none

Citation: Wang Yu,Xu Guifang,Zhang Qingrong.Electrochemiluminescent immunosensor based on magnetic nanofibers/carbon nanohorns for ultrasensitive detection of aflatoxin B1[OL]. [10 September 2015] http://en.paper.edu.cn/en_releasepaper/content/4653712

A label-free electrogenerated chemiluminescence (ECL) immunoassay protocol was fabricated based on magnetic nanoarchitecture for sensitive detection of low-abundance aflatoxin B1 (AFB1). It was constructed by combining the Fe3O4 nanoparticles doped magnetic nanofibers (Fe3O4-NFs) with carbon nanohorns (CNHs) onto the magnetic glassy carbon electrode (MGCE). Owing to the specific recognition of antibody with AFB1 antigens and the effective ECL amplification of the nanoarchitecture, the proposed immunosensor shows high sensitivity and specificity and responds linearly to the concentration of AFB1 from 0.05 ng/mL to 200 ng/mL with a low detection limit of 0.02 ng/mL. Moreover, the immunosensor successfully achieves the detection of AFB1 in practical samples.

Keywords:electrochemiluminescence; immunosensor; aflatoxin B1; magnetic nanofibers

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