A innovative electrochemiluminescent immunosensor based on carbon nanodots functionalized  with  poly(amidoamine) dendrimer for  α-fetoprotein detection

Chen Sihong; Zhang Shupei; LI Yilin; Gong Lingshan; Yan Huixiang; Zhang Qingrong; Xu Guifang; Dai Hong

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A innovative electrochemiluminescent immunosensor based on carbon nanodots functionalized with poly(amidoamine) dendrimer for α-fetoprotein detection

Chen Sihong #,Zhang Shupei,LI Yilin,Gong Lingshan,Yan Huixiang,Zhang Qingrong,Xu Guifang,Dai Hong *

College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350007

*Correspondence author

#Submitted by

Subject:

Funding: Education Department of Fujian Province（No.JA14071, JB14036, JA13068）, NSFC （No.21205016）, Fujian Normal University outstanding young teacher research fund projects （No.fjsdjk2012068）, National Science Foundation of Fujian Province（No.2011J05020）, Foundation of Fuzhou Science and Technology Bureau（No.2013-S-113）

Opened online: 7 March 2016

Accepted by: none

Citation: Chen Sihong,Zhang Shupei,LI Yilin.A innovative electrochemiluminescent immunosensor based on carbon nanodots functionalized with poly(amidoamine) dendrimer for α-fetoprotein detection[OL]. [ 7 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4679177

A sandwich-type electrochemiluminescent biosensor based on carbonaceous based nanomaterials was fabricated for alpha-fetoprotein detection. After the capturing antibody was immobilized onto the modified electrode surface, the detection antibody together with poly(amidoamine) dendrimers functionalized carbon nanodots as the recognizable signal tags was self-assembled onto the electrode by typical sandwich immune reaction. Poly(amidoamine) dendrimers functionalized carbon nanodots make the composite not only an immobilizing platform for carrying numerous detection antibodies, but also an promising promoter for the electrochemiluminescent signal of luminol.This immunosensing strategy for alpha-fetoprotein with the assistance of poly(amidoamine) dendrimers functionalized carbon nanodots provided a promising approach in clinical disease scanning.

Keywords:electrochemiluminescent; biosensor; alpha-fetoprotein; carbon nanodots;poly(amidoamine) dendrimers

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