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Development of a LC-MS/MS Method for Determination of Triptolide in Mouse Plasma: Application to Potential Drug-drug Interaction
ZHANG Junying 1,LIU Xiaoying 2,WU Chunyong 2 * #
1.School of Traditional Chinese Pharmacy,China Pharmaceutical University, Nanjing 211198
2.School of Pharmacy, China Pharmaceutical University, Nanjing 210009
*Correspondence author
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Funding: 高等学校博士学科点专项科研基金（No.20120096120004）
Opened online:19 May 2016
Accepted by: none
Citation: ZHANG Junying,LIU Xiaoying,WU Chunyong.Development of a LC-MS/MS Method for Determination of Triptolide in Mouse Plasma: Application to Potential Drug-drug Interaction[OL]. [19 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4689876
 
 
A simple, sensitive, and accurate LC-MS/MS method for determining triptolide in mouse plasma has been developed and validated to study the drug-drug interaction with curcumin. The quantification was performed with the transitions of [M+NH4]+→[M+H]+ for triptolide. The assay for triptolide in mouse plasma was linear over the range of 0.25 to 100 ng/mL and the Lower Limit of Quantification was 0.25 ng/ml. After a single oral administration of triptolide at 0.5 mg/kg, triptolide reached the maximum exposure at 11.64 ± 4.08 min, and declined rapidly with a mean elimination half-life of 38.52 min. Co-administration of 400 mg/kg curcumin significantly increased Cmax and AUC0-∞ by 1.8-fold and 1.5-fold respectively, demonstrating that curcumin increases the bioavailability of triptolide in mouse.
Keywords:Pharmaceutical analysis; Triptolide; LC-MS/MS; Pharmacokinetics; Drug-drug interaction; Curcumin
 
 
 

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