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| Objective. To investigate SKOV3 cells growth induced by malignant ascites whether to involve in nuclear factor kappaB (NF-κB) signal transduction pathway. Methods. Expression of NF-κBp65 and IκBα protein of SKOV3 cells stimulated by malignant ascites, benign and borderline cystic fluid are measured by immunocytochemistry and western blotting analysis. Results. SKOV3 growth rate in the malignant ascites group (217.24±12.64) is significantly higher than that of the borderline fluid group (147.67±10.43, P=0.000), the borderline fluid group higher than the benign fluid group (57.47±2.30, P=0.000). Immunohistochemical and western blotting analyzing study show that NF-κBp65 and IκBα protein are localized in cytoplasm and karyon. Nuclear-cytosol NF-κBp65 and cytosol NF-κBp65/IκBα protein ratio in the malignant group (12.71±1.70, 3.50±0.74, respectively) is higher than that of the borderline group (2.89±0.84, P=0.000; 1.88±0.44，P=0.015, respectively), as well as the benign group (1.64±0.61, P=0.000; 1.04±0.31，P=0.001, respectively) and the control group (1.23±0.45, P=0.000; 0.69±0.09, P=0.001, respectively). There is a positively correlated relationship between expression of NF-κBp65 and IκBα (correlated coefficient 0.839, P=0.001), between SKOV3 growth rate and nuclear-cytosol NF-κBp65 protein ratio(correlated coefficient 0.878，P=0.000) as well as cytosol NF-κB/IκBα protein ratio (correlated coefficient 0.839，P=0.001).
Conclusion. Malignant ascites may activate NF-κB and promote cells proliferation of human ovarian carcinoma SKOV-3 cells. |
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| Keywords: ovarian carcinoma; ascites; NF-κBp65;IκBα |
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