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Maternal Subclinical Hypothyroidism Can Affect Hippocampi BDNF and Rap1 Expression in Rat Offspring
YU Xiaohui 1,LIU Dijie 2,SHAN Zhongyan 2 *,TENG Weiping 2
1.Department of Endocrinology and Metabolism, The First Hospital of China medical University, Key Laboratory of Endocrine Diseases of Liaoning Province, ShenYang 110001
2.Department of Endocrinology and Metabolism, The First Hospital of China medical University, Key Laboratory of Endocrine Diseases of Liaoning Province, Shenyang 110001
*Correspondence author
#Submitted by
Subject:
Funding: the Key Laboratory Support Special Program of Liaoning Province（No.20070905）, the Healthcare Industry Special Project（No.200802008）, Special Research Fund for Doctor Discipline Unit of the Ministry of Education （No.No. 20092104120003）, the Social Development Foundation of Liaoning Province（No.[2005]36–39）
Opened online:30 November 2012
Accepted by: none
Citation: YU Xiaohui,LIU Dijie,SHAN Zhongyan.Maternal Subclinical Hypothyroidism Can Affect Hippocampi BDNF and Rap1 Expression in Rat Offspring[OL]. [30 November 2012] http://en.paper.edu.cn/en_releasepaper/content/4497524
 
 
Background: Epidemiological studies and case reports have shown that maternal subclinical hypothyroidism may result in significant negative effects on pregnancy and neurodevelopment of the fetus. To understand the mechanism responsible for these neurological alterations, we induced maternal subclinical hypothyroidism in pregnant rats. several genes that are under the control of THs were evaluated in the offspring of TH-deficient rats. Methods: A total of 60 female rats were divided into three groups: (i) maternal subclinical hypothyroidism (total thyroidectomy with T4 infusion), (ii) maternal hypothyroidism (total thyroidectomy without T4 infusion), and (iii) control (sham operated). All rats were mated 10 days after the start of infusion. The infusion continued until 10 days postpartum. Pups were sacrificed at postnatal day 3 (PND 3), PND 7, and PND 21. The hippocampus was collected and tested for brain-derived neurotrophic factor (BDNF) and Rap1 protein expression by Western blotting and for BDNF and neural cell adhesion molecule mRNA expression by real-time polymerase chain reaction. Results: This study found decreases in BDNF mRNA expression (on PND 3) and protein level (on PND 3 and PND 7) in hippocampi of pups from subclinical hypothyroidism dams ( p<0.05). Rap1 protein expression was higher in maternal subclinical hypothyroidism offspring than in control offspring at PND 7 and PND 21. No change was observed in neural cell adhesion molecule mRNA expression in the maternal subclinical hypothyroidism offspring. Conclusions: Maternal subclinical hypothyroidism dams can affect the expression of brain development related genes in pups. It can decrease BDNF mRNA expression and protein level as well as increase Rap1 protein expression in hippocampi.
Keywords:Internal medicine; subclinical hypothyroidism; brain development; pregnancy
 
 
 

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