Enhance eicosapentaenoic acid production in oleaginous fungus Mortierella alpina by overexpressing ω3 fatty acid desaturase

Xiaoyun Huang; Haiqin Chen; Guangfei Hao; Kai Du; Danhui Hao; Yuanda Song; Zhennan Gu; Hao Zhang; Wei Chen; Yong Q. Chen

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Enhance eicosapentaenoic acid production in oleaginous fungus Mortierella alpina by overexpressing ω3 fatty acid desaturase

Xiaoyun Huang 1 #,Haiqin Chen 1,Guangfei Hao 2,Kai Du 1,Danhui Hao 1,Yuanda Song 1,Zhennan Gu 1,Hao Zhang 1,Wei Chen 2,Yong Q. Chen 1 *

1.State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, P.R. China

2. Synergistic Innovation Center for Food Safety and Nutrition, Wuxi 214122, P.R. China

*Correspondence author

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Funding: National Natural Science Foundation of China （No.No. 21276108, 31271812), Fundamental Research Funds for the Central Universities）

Opened online:25 March 2014

Accepted by: none

Citation: Xiaoyun Huang,Haiqin Chen,Guangfei Hao.Enhance eicosapentaenoic acid production in oleaginous fungus Mortierella alpina by overexpressing ω3 fatty acid desaturase[OL]. [25 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4590491

ω3 fatty acid desaturase is the key enzyme responsible for ω-3 polyunsaturated fatty acids (PUFAs) formation. In oleaginous fungus Mortierella alpina, about 50% of total fatty acids are arachidonic acid (AA), whereas ω-3 PUFAs level is very low. In order to enhance the eicosapentaenoic acid (EPA) production, ω3 fatty acid desaturase gene (FADS15) from M. alpina ATCC32222 was cloned and homologously overexpressed, using the Agrobacterium tumefaciens-mediated transformation. Compared to the wild-type M. alpina, the FADS15 overexpressing strains MA-ω3Des-1, MA-ω3Des-2, MA-ω3Des-3 had 6.35-, 5.33- and 6.27-fold increase in EPA yield, respectively. These FADS15 overexpressing clones may be useful to future industrial production of EPA.

Keywords:Mortierella alpina; ω3 fatty acid desaturase; overexpression; EPA production

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