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| Cardiovascular diseases are currently the major causes of mortality in the world, especially in developed nations. As a predominant one, thrombosis is the platelet aggregation induced by a high shear rate. Platelet aggregation assay can clarify the occurrence mechanism of thrombosis, as well as used as an important tool in the clinical diagnosis, personalized treatment, and screening of anticoagulants. Thus, relevant studies attracted considerable attention. As an important step in platelet aggregation, platelet adhesion and its detection are also attracted intensive concern. Thus, some analytical methods have been developed for platelet adhesion assay, and the impact of shear rate is one of the focuses. Compared with other devices, biosensors can give a more accurate result within a shorter time. Furthermore, some biosensors can achieve real-time analysis. However, only one or several shear rates can be tested at the same time, which may decrease the analytical efficiency. Meanwhile, in most cases, only the average platelet-adhesion effect within a reactor is detection, and the impact of the distribution of shear rates is improperly neglected.
In this study, a microfluidic device with a single channel is designed and fabricated for platelet adhesion assay. When the platelet-rich plasma flows through the collagen-modified sensing surface of the channel bottom, the interaction between platelets and collagen molecules on the entire surface can be simultaneously monitored by using a surface plasmon resonance imaging (SPRi) system. A gradient of the shear rate (0-546) could be formed within the channel by choosing a suitable depth-to-width ratio (1:5), so platelet adhesion at multiple shear rates could be monitored simultaneously. This method enables the measurement of the adhesion process of unlabeled platelets on the entire sensing surface, in vitro, at multiple shear rates. Such a system can obtain more accurate platelet adhesion result at a given shear rate than traditional methods. Furthermore, in an individual operation, platelet adhesion can be repeatedly tested at multiple points with an equal shear rate, so a much higher analytical efficiency can also be achieved. |
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| Keywords:Biomedical engineering; Surface plasmon resonance imaging; Platelet aggregation; Biosensor; Microfluidics |
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