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1. Tyrosine hydroxylase (TH) upstream-inhibited UPF3 regulator of nonsense transcripts homolog A (UPF3A) subnetwork for learning in human left hemisphere|Prostate via nucleus to mitochondrion to cytosol RNA binding | |||
Xi Ruipeng,HUANG Juxiang,WANG Lin | |||
Biology 14 September 2020 | |||
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Abstract:High tyrosine hydroxylase (TH) upstream-inhibited UPF3 regulator of nonsense transcripts homolog A (yeast) (UPF3A) molecular subnetwork was constructed, including upstream hypothetical LOC400642 (LOC400642), retinoblastoma binding protein 6 (RBBP6), ubiquitin protein ligase E3 component n-recognin 5 (UBR5); downstream chromosome 10 open reading frame 10 (C10orf10), sulfotransferase family 1A member 2 (SULT1A2) reported relation with learning in human left hemisphere. The common biology process of TH upstream-inhibited UPF3A subnetwork was identified by DAVID, containing upstream RBBP6, upstream UBR5, downstream SULT1A2, second-core UPF3A, first-core TH as protein binding; upstream RBBP6, upstream UBR5 as ubiquitin protein transferase activity, zinc ion binding, ligase activity, cellular response to DNA damage stimulus, protein ubiquitination involved in ubiquitin dependent protein catabolic process; upstream UBR5, second-core UPF3A as RNA binding; downstream SULT1A2, first-core TH as small molecule metabolic process; The common cellular component of upstream RBBP6, upstream UBR5, second-core UPF3A, first-core TH as cytoplasm; upstream RBBP6, upstream UBR5, second-core UPF3A as nucleoplasm; upstream UBR5, second-core UPF3A, first-core TH as nucleus; downstream SULT1A2, second-core UPF3A, first-core TH as cytosol; downstream C10orf10, first-core TH as mitochondrion; The common tissue distributions as Prostate_3rd maybe exist the same pattern with human left hemisphere. We propose and mutual positively verify tyrosine hydroxylase (TH) upstream-inhibited UPF3 regulator of nonsense transcripts homolog A (yeast) (UPF3A) subnetwork for learning in human left hemisphere|Prostate via nucleus to mitochondrion to cytosol RNA binding. | |||
TO cite this article:Xi Ruipeng,HUANG Juxiang,WANG Lin. Tyrosine hydroxylase (TH) upstream-inhibited UPF3 regulator of nonsense transcripts homolog A (UPF3A) subnetwork for learning in human left hemisphere|Prostate via nucleus to mitochondrion to cytosol RNA binding[OL].[14 September 2020] http://en.paper.edu.cn/en_releasepaper/content/4752808 |
2. High efficiency of neuron differentiation from neural stem cells by monolayer culturing by mash1> | |||
Zhang Qian,Zhou Mingming,Wu Xiaobo,Zhang Jie,Cao Yuelong,Ding Daofang,Zhan Hongsheng | |||
Biology 10 July 2013 | |||
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Abstract:Efficient generation of neuroprogenitor or neurons is necessary for the development of autologous cell therapies for the treatment of neurological disease. Neural stem cells (NSCs) represent a valuable resource for both research and regenerative medicine due to their ability to propagate indefinitely in vitro and to spontaneously differentiate into neurons. However, several weeks are often required for neuronal generation, and the percentage of resulting neurons is often relatively low. In this study, we adopted the initiative methods in China. Primary neurosphere monolayer from rat EC stem cells was isolated by selecting a single colony during passage, more than 95% of neural stem cells formed the neurosphere monolayer only after 1 day of differentiation, and purified homogenous NSCs proliferated throughout the entire plate after six passages. This monoclonic culture assay on neurosphere monolayer successfully generated high-contented neuron with high purity. In addition, the effect of serum concentration on neuronal production was also characterized in this study. These novel methods will be valuable for both research purposes as well as for clinical therapies. | |||
TO cite this article:Zhang Qian,Zhou Mingming,Wu Xiaobo, et al. High efficiency of neuron differentiation from neural stem cells by monolayer culturing by mash1>[OL].[10 July 2013] http://en.paper.edu.cn/en_releasepaper/content/4550749 |
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