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	1. Construction of a genetic map using newly developed SSR markers for identifying QTL for plant height in jute (Corchorus capsularis)
	ZHANG Liwu
	Agronomy 27 March 2016
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	Abstract:Jute, comprising white and dark jute, is one of the most important crops for natural ?ber production. In this study, a set of 197 novel SSRs from 529 publicly available dark jute genomic sequences were developed and used to assess polymorphism among six diverse accessions of dark and white jute. Of these SSRs, 142 (72.1%) successfully amplified at least one fragment and 122 (61.9%) detected polymorphism, showing the high transferability of these dark jute SSRs to white jute.An SSR genetic map of white jute using an F8 recombinant inbred lines population consisting of 94 lines derived from a cross between Huangma 179 and Aidianyehuangma was constructed. The map, comprising 39 newly developed SSR markers and spanning 389.9 cM with an average distance of 12.1cM between adjacent markers, was used for identifying quantitative trait loci (QTL) for plant height. Six QTL were detected, of which qPH.N1 was stable in different environments and regarded as a major QTL. This study has not only enriched the stock of jute SSR markers but also shed light on the genetic basisof plant height at the QTL level in jute.
	TO cite this article:ZHANG Liwu. Construction of a genetic map using newly developed SSR markers for identifying QTL for plant height in jute (Corchorus capsularis)[OL].[27 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4682339


	2. Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in cultivated Brassica napus
	HENG Shuangping,CHEN Fengyi,YANG Zonghui,HU Kaining,WEI Chao,WEN Jing,YI Bing,MA Chaozhi,TU Jinxing,SI Ping,FU Tingdong,SHEN Jinxiong
	Agronomy 30 September 2015
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	Abstract:Mitotype-specific markers have been developed in order to distinguish among different mitotypes in plant. Here, we comparatively analyzed six sequenced mitochondrial genomes that are studied in Brassica napus and identified the collinear blocks and mitotype-specific sequences (MSSs) of these mitochondrial genomes. The collinear relationship between mitochondrial genomes of nap, cam, and pol cytoplasmic male sterility (CMS) lines were higher than those of other lines. After comparative analysis of the six sequenced mitochondrial genomes (cam, nap, ole, pol CMS, ogu CMS, and hau CMS), 90 MSSs with sizes ranging from 101 to 9981 bp and a total length of 103,756 bp (accounting for 6.77% of the mitochondrial genome sequences) were identified. Additionally, 12 mitotype-specific markers were developed based on the mitochondrial genome specific sequences in order to distinguish among these different mitotypes. Cytoplasms of 570 different inbred lines collected from various scientific research institutes in China were identified using the MSS markers developed in our study. In addition to confirming the accuracy of the cytoplasmic identification, we also identified mitotypes that have not been reported in Brassica napus. Our study may provide guidance for the classification of different mitotypes in B. napus breeding.
	TO cite this article:HENG Shuangping,CHEN Fengyi,YANG Zonghui, et al. Identification of different cytoplasms based on newly developed mitotype-specific markers for marker-assisted selection breeding in cultivated Brassica napus[OL].[30 September 2015] http://en.paper.edu.cn/en_releasepaper/content/4656522


	3. iTRAQ -based quantitative proteomics analysis of upland cotton (Gossypium hirsutum) stem terminal buds reveals phytohormone related pathways associated with dwarfism
	TU Xiaoju,WANG Qiming,LI Juan,LIU Aiyu
	Agronomy 26 July 2015
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	Abstract:Identifying important regulative elements and pathways related to dwarf in cotton is a major challenge in cotton breeding. An iTRAQ-based proteomics of stem terminal buds from the upland cotton species Gossypium hirsutum dwarf line LA-1 and high near-isogenic line （NIL） LH-1 was performed. Moreover, the transcriptional expression of differentially expressed proteins (DEPs) belonged to phytohormone signal transduction, hormone biosynthesis and ubiquitin system, were analyzed using the quantitative real-time polymerase chain reaction (PCR). A total of 4, 849 proteins were identified from LA-1 and LH-1, 697 of which showed differential accumulations. Most of DEPs function as catalytic activity, binding and transporter activity, and involved in metabolism and protein processing related pathways. In particular, 7 DEPs, including 2 of gibberellins (GA) receptor, 3 of cytokinin (CK) receptor, CK oxidase and CK-N-glucosyltransferase were up-regulated in LA-1, and gibberellin 20-oxidase was down-regulated in LA-1. Our results suggest that DELLA-independent GA signaling pathway is primary reason caused the dwarf in LA-1, and indicate that CRE1-2, GID, CKX and GA20ox are potential indicators of dwarf cotton, and the profiling of DEPs may offer a valuable resource for molecular markers of dwarf cotton breeding.
	TO cite this article:TU Xiaoju,WANG Qiming,LI Juan, et al. iTRAQ -based quantitative proteomics analysis of upland cotton (Gossypium hirsutum) stem terminal buds reveals phytohormone related pathways associated with dwarfism[OL].[26 July 2015] http://en.paper.edu.cn/en_releasepaper/content/4650289

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