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1. Genetic diversity of Hirsutella sinensis revealed by EST-SSR | |||
YUAN Feng,YU Hong,ZUO Shimei | |||
Biology 19 November 2014 | |||
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Abstract:In recent years, expressed sequence tags has been used as a new approach to develop markers, as they rapidly accumulated in databases. In the present study, EST-SSR markers of the genus Hirsutella have been identified using primers designed from ESTs pools of Paecilomyces militaris and were found to have well transferability from Paecilomyces to Hirsutella. These markers were used to evaluate genetic diversity and structure within and between 8 populations of Hirsutella sinensis. The genetic differentiation of H. sinensis was relatively astonishing between populations (67.49%). The Nm was 0.2408, indicating serious lack of gene flow among populations of H. sinensis. The genetic distance and geographical distance were significantly positively correlated relationship (P = 0.04). And the analysis of mismatch distribution indicated the populations of H. sinensis underwent population expansion recently. It was also found that H. robertsii might originate from H. sinensis. The EST-SSR markers were proved to be an informative and useful tool for assaying genetic diversity and authenticating species of Hirsutella. | |||
TO cite this article:YUAN Feng,YU Hong,ZUO Shimei. Genetic diversity of Hirsutella sinensis revealed by EST-SSR[OL].[19 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4619402 |
2. Genetic Structure of Paecilomyces militaris by EST-SSR | |||
Feng Yuan,Hong Yu,Shi Mei Zuo | |||
Biology 19 November 2014 | |||
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Abstract:The EST-SSR markers of Paecilomyces genus was established with the primers designed according to ESTs pools of Paecilomyces militaris. The markers had well transferability to Paecilomyces hepialid. Genetic diversity and structure were also evaluated within and among 11 populations of P. militaris. Nine primer groups were screened, and a total of 179 DNA fragments were amplified, all of which were polymorphic, averaging 19.9 polymorphic bands per primer. At the species level, the percentage of polymorphic bands was 100.00%, and 28.08% within the 11 populations. The observed number of alleles (Na) was 2.0000 and the effective number of alleles (Ne) was 1.3278; Nei's gene diversity (H) was 0.2128; Shannon's information index (I) was 0.3452. At the population level, Na = 1.3017, Ne = 1.1677, H = 0.0918, and I = 0.1546. Total gene diversity was 0.2027(Ht), the gene diversity within population was 0.0918(Hs), the coefficient of gene differentiation was 0.5472(Gst), and estimated gene flow was 0.4138(Nm). These results indicated the genetic differentiation was relatively higher among populations of P. militaris. The genetic structure had correlation with the geographical source, but not so obvious among the populations in Yunnan. The Paecilomyces sp. isolated from living Hepialus yunnanensis was proved to be Paecilomyces hepialid. The EST-SSR markers were an informative and useful method for assaying genetic diversity and authenticating species of Paecilomyces. The EST-SSR markers would provide for insight into population genetics of Paecilomyces species and help us select genotypes with high adaptability and high content of useful chemical components. | |||
TO cite this article:Feng Yuan,Hong Yu,Shi Mei Zuo. Genetic Structure of Paecilomyces militaris by EST-SSR[OL].[19 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4619399 |
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