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	1. Antioxidative effect of Oolong tea Polysaccharides on Streptozotocin induced diabetic rats
	YU zhi,ZHANG yun,NI dejiang,ZHOU JIRONG
	Agronomy 28 February 2013
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	Abstract:Effect of Oolong tea polysaccharide (OTPS) on the antioxidant and antidiabetic activities in streptozotocin-induced diabetic rats were investigated. Significant changes of biochemical were found in serum and tissues 30 days after OPTS-treated diabetic rats induced by a single dose of streptozotocin. OTPS significantly reduced blood glucose (BG), nitric oxide (NO) and malondialdehyde (MDA) concentration and activity of nitric oxide synthase (NOS) in serum of diabetic rats, meanwhile the activities of superoxide dismutase(SOD), glutathione peroxidase (GSH-PX), and the content of insulin in serum were increased.
	TO cite this article:YU zhi,ZHANG yun,NI dejiang, et al. Antioxidative effect of Oolong tea Polysaccharides on Streptozotocin induced diabetic rats[OL].[28 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4524853


	2. Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua
	Liang Yuerong,Lin Chen,Wang Xunlei,Ye Jianhui,Lu Jianliang
	Agronomy 25 January 2012
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	Abstract:Ectropis obliqua is a major tea pest and chitin synthase (CHS) plays a key role in the pest growth and development. A 192 bp conserved domain from E. obliqua CHS gene was cloned and it was used to construct recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) with double-stranded RNA interference (dsRNAi) method. The recombined AcMNPV virus could propagate in host cells sf9. Injection test showed that the virus efficacy of the recombined AcMNPV on E. obliqua larvae was significantly enhanced. It is considered that the CHS dsRNAi mediated by the Nuclear polyhedrosis virus will be interesting for development of alternative bio-pesticide to control the tea pest E. obliqua.
	TO cite this article:Liang Yuerong,Lin Chen,Wang Xunlei, et al. Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua[OL].[25 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463882


	3. Chitin synthase (CHS) gene in epidermis of Ectropis obliqua Prout
	Liang Yuerong,Lin Chen,Wang Luoren,Ye Jianhui,Lu Jianliang
	Agronomy 25 January 2012
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	Abstract:Ectropis obliqua Prout is a major pest in tea fields and chitin synthase (CHS) plays an important role in biosynthesis of chitin and growth of the pest. A cDNA sequence encoding the CHS and its expression pattern during development of E. obliqua were investigated. The CHS cDNA sequence was 5496 bp nucleotides, with an open reading frame of 4692 bp encoding a protein of 1563 amino acids. It belonged to CHS-A member of CHS gene family. The CHS-A expression was the strongest in the third and fourth instar larvae, during which the growth rate of E. obliqua larvae was the rapidest. Catalysis model of CHS-A enzyme in E. obliqua was also hypothesized according to the specific motifs and topological structure prediction of the protein. This study provided an important information for the further research on development of RNA interference (RNAi) technology to control E. obliqua.
	TO cite this article:Liang Yuerong,Lin Chen,Wang Luoren, et al. Chitin synthase (CHS) gene in epidermis of Ectropis obliqua Prout[OL].[25 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4463876


	4. Light-induced gene expression involved in phenylpropanoid biosynthetic pathways in callus of tea (Camellia sinensis L.)
	Wang YunSheng ,Gao LiPing ,Wang ZhengRong ,Liu YaJun ,Sun MeiLian ,Yang DongQing ,Wei Chaoling ,Shan Yu ,Luo Yang ,Xia Tao
	Agronomy 11 March 2010
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	Abstract:Tea (Camellia sinensis L.) is a commercially important crop that is valued for its secondary metabolites. Light can influence the accumulation of phenylpropanoids (including catechins, anthocyanins, and lignins) in the seedlings of tea plants. To investigate the molecular mechanism by which light influences phenylpropanoid metabolism, we established light-induced suppression subtractive hybridization (SSH) cDNA libraries of tea callus. A total of 191 clones from the forward (light-induced) SSH library and 74 clones from the reverse (darkness control) library were selected, sequenced, and analyzed in this study. BlastX analysis of the tea callus light-induced cDNA library showed that the subtractive expressed sequence tags (ESTs) were classified into 12 putative cellular functions, in which the ‘photomorphogenesis’, ‘stress defense’, ‘signal transduction’, and ‘phenylpropanoid and secondary metabolism’ categories contained 5, 17, 13, and 9 ESTs, respectively. The bioinformatic sequence analysis of the SSH library, associated with morphological comparison, secondary metabolites analysis, and representative genes expression analysis, implied that: (1) light could inhibit the growth, and promote the differentiation, of the tea callus; (2) light might be effective for activation of the biosynthesis of phenylpropanoids, e.g. analysis and identification of catechins implied that light irradiation could activate epicatechin and epigallocatechin biosynthesis; and (3) light, as a signal or a stress condition, may regulate photomorphogenesis and phenylpropanoid synthesis mediated by the cAMP and Ca2+ signal pathway in tea callus. The SSH library established in this study represents a valuable resource for future research aimed at improving economically important secondary metabolic characteristics in the tea plant.
	TO cite this article:Wang YunSheng ,Gao LiPing ,Wang ZhengRong , et al. Light-induced gene expression involved in phenylpropanoid biosynthetic pathways in callus of tea (Camellia sinensis L.)[OL].[11 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40595


	5. Isolation and Preliminary Identification of Aluminium-resistant Microorganisms from the Rhizophric Soil of Tea Plant
	Wu Ying,Liang Yuerong,Lu Jianliang,Liu Zusheng
	Agronomy 03 March 2006
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	Abstract:Microorganism isolated from rhizospheric soil of tea plant was inoculated in culture medium containing aluminium 5-20 mmol/L and an aluminium-resistant fungus ALF-1 was isolated. The ALF-1 fungus possesses aluminium-resistant ability. It could grow normally in the medium containing 20 mmol/L of aluminium and decreased the aluminium content in medium and faded the haematocylin colour of the medium. The fungus was identified as a species of Neurospora sp.
	TO cite this article:Wu Ying,Liang Yuerong,Lu Jianliang, et al. Isolation and Preliminary Identification of Aluminium-resistant Microorganisms from the Rhizophric Soil of Tea Plant[OL].[ 3 March 2006] http://en.paper.edu.cn/en_releasepaper/content/5494

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