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| There are 179 papers published in subject: since this site started. | |||
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| 1. OAS1 network construction and analysis of the frontal cortex with HIV encephalitis (HIVE) compared with no-encephalitis HIV patients by integrative biocomputation | |||
| LI Hao,WANG Lin,HUANG Juxiang | |||
| Basic Medicine 14 November 2018 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (867K B) | |||
| Abstract:Single molecular functional network construction and analysis of disease is very useful to identify novel and potential targets for prognosis and therapy. This paper integrated an infer method based on linear programming and decomposition procedure with function analysis using Kappa statistics and fuzzy heuristic cluster (DAVID). We first identified the significant molecule OAS1, then constructed OAS1 up- and down-stream network by infer and further data-mined the main OAS1 modules including response to stimulus, catalytic activity, organelle, metabolic process, alternative splicing and sequence variant from 16 frontal cortex of HIV encephalitis (HIVE) and 12 no-encephalitis HIV patients in the same GEO Dataset GDS1726. Our infer OAS1 network result showed the different gene rate of HIVE as 78% (21/27) compared with the control considering activation and inhibition relationship. The different active genes in HIVE include HLA_B, ISG15_1, ISG15_2, LCAT, LY96, M33210, PDCD4, STAT1, VEZF1, ADH1B, DGKG, IFI35, LSM7, TSPAN4, ZC3HAV1 and the different inhibitory genes include DGKG, CREB5, GAS1, M33210, TENC1, VEZF1. Our integrative analysis showed the positive results of OAS1 response to stimulus, catalytic activity, organelle, metabolic process, alternative splicing and sequence variant through the net numbers of activation minus inhibition compared with the control and predicted the increases of these modules in HIVE. | |||
| TO cite this article:LI Hao,WANG Lin,HUANG Juxiang. OAS1 network construction and analysis of the frontal cortex with HIV encephalitis (HIVE) compared with no-encephalitis HIV patients by integrative biocomputation[OL].[14 November 2018] http://en.paper.edu.cn/en_releasepaper/content/4746438 | |||
| 2. Reduced Nicotinamide Adenine Dinucleotide Phosphate Inhibits Platelet Function | |||
| Gu Yi,Qin Zhenghong | |||
Basic Medicine 07 March 2018
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (813K B) | |||
| Abstract:Background-In previous studies, the protection of reduced nicotinamide adenine dinucleotide phosphate (NADPH) on neurons against ischemia/reperfusion-induced injury was determined and its inhibition on platelet aggregation was found by accident. Here, the function of NADPH on platelet is explored deeply. Methods and Results-In vitro studies, the effects of different concentrations of NADPH on platelet aggregation induced by ADP (10 μM), thrombin (0.05 U/mL) or AA (0.5 mM) were determined and we found that NADPH could concentration-dependently inhibit platelet aggregation induced by ADP or thrombin. When the inhibitory effects of NAD+, NADH, NADP+ and NADPH on platelet aggregation were compared, NADPH showed the best effect. In vivo studies, the effects between NADPH and aspirin on tail bleeding time, clotting time and ferric chloride-induced thrombosis model were compared in mice or rats. NADPH could prolong the tail bleeding times in mice, especially at 30 min after the injection of NADPH (7.5 mg/kg), while bleeding times in mice given aspirin (15 mg/kg) were significantly longer at any time point in 6 h. As we detected the influence of NADPH or aspirin on clotting times in rats, we found that both of them had no effect. Using a FeCl3-induced abdominal aorta injury thrombosis model, we found that the injection of NADPH provoked a significant time delay of vessel occlusion, while aspirin almost completely prevented the vessel occlusion. After we observed the injured vessel sections under the microscope, we discovered that the thrombi in injured vessel sections of rats injected NADPH were much looser than rats injected normal saline and the thrombi in injured vessel sections of rats given aspirin were fewest. Conclusions-The current results suggest that NADPH can inhibit platelet aggregation and prevent hemostasis and thrombosis. To our knowledge, this is the first study on the effect of NADPH on platelet function. | |||
| TO cite this article:Gu Yi,Qin Zhenghong. Reduced Nicotinamide Adenine Dinucleotide Phosphate Inhibits Platelet Function[OL].[ 7 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4743750 | |||
| 3. Dental Pulp Stem Cells in Tooth Regeneration: Characterizations and Applications | |||
| Su Cheng,Zhu Guanyin,Zheng Yi,Gao Bo | |||
| Basic Medicine 04 November 2017 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (530K B) | |||
| Abstract:In order to make up the deficiency of traditional medical treatment method, regenerative medicine as a new research field has received extensive attention in the recent years. Dental pulp stem cells(DPSCs), originating from the neural crest, become a promising source for tooth regeneration applications for their unique advantages, such as easily isolated and minimally invasive. As a kind of mesenchymal stem cells, DPSCs have the ability to differentiate in several cell phenotypes including odontogenic differentiation, vascularization and neural differentiation. To be applied in tissue engineering, DPSCs are usually combined with various scaffolds and growth factors. Based on the characterizations of DPSCs, they are considered to be a great candidate for application used in regenerative endodontic procedures and whole tooth regeneration. In this review, we have carefully described the important aspects of DPSCs and discussed the practical applications in the present studies. this paper. | |||
| TO cite this article:Su Cheng,Zhu Guanyin,Zheng Yi, et al. Dental Pulp Stem Cells in Tooth Regeneration: Characterizations and Applications[OL].[ 4 November 2017] http://en.paper.edu.cn/en_releasepaper/content/4741871 | |||
| 4. Characterization of HIV-1 Variants Resistant to an Electronically Constrained Peptide Fusion Inhibitor | |||
| Xiyuan Wu | |||
| Basic Medicine 04 May 2017 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (4K B) | |||
| Abstract:Emergence of drug resistant HIV-1 mutants is a serious problem for AIDS treatment. SC29EK, a shorter variant of SC34EK, is one of the representative next-generation fusion inhibitors of HIV-1. It shows high activity on inhibiting both wide type and T-20 resistant HIV-1 strains. In this study, we focused on the selection and characterization of HIV-1 escape mutants of SC29EK. Three mutations, Q39R, N43K and E49A located at N-heptad repeat regions and N126K located at C-heptad repeat region of gp41 were identified. High resistance to pocket targeting inhibitor peptides and cross-resistance to enfuvirtide (T20) and sifuvirtide (SFT) were detected, with the mechanisms of significantly reducing binding affinity of the inhibitors and dramatically enhancing interaction of the viral six-helix bundle. Our data helps increase the understanding of the structure-function relationship of gp41 and HIV-1 evolution, providing a reference for the clinical application of fusion inhibitors.????? | |||
| TO cite this article:Xiyuan Wu. Characterization of HIV-1 Variants Resistant to an Electronically Constrained Peptide Fusion Inhibitor[OL].[ 4 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4732446 | |||
| 5. Development of Fast Scanning Photoacoustic/Ultrasound Microscopy based on Parablic Mirror | |||
| WU Ning,LI Changhui | |||
| Basic Medicine 04 May 2017 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (4K B) | |||
| Abstract:We have developed a prototype fast scanning photoacoustic/ultrasound dual-modality microscopy, which provides the complementary information of optics and ultrasound imaging. Based on the design of optical parabolic mirror and water-immersible MEMS scanning mirror, the system achieves fast scanning property as well as confocal effect of collimated light and parallel ultrasound, to acquire a high resolution and simplified configuration. The feasibility of this system is demonstrated by the phantom experiment. The system shows strong potential for the pre-diagnosis of significant diseases. | |||
| TO cite this article:WU Ning,LI Changhui. Development of Fast Scanning Photoacoustic/Ultrasound Microscopy based on Parablic Mirror[OL].[ 4 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4732388 | |||
| 6. Ginsenoside-Rh2 suppresses proliferation and migration of hepatocellular carcinoma cells by targeting EZH2 to regulate CDKN2A-2B gene cluster transcription | |||
| Li Qi | |||
| Basic Medicine 25 April 2017 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (4K B) | |||
| Abstract:Ginsenoside-Rh2 (G-Rh2) exerts important pharmacological effects with regard to the control of human hepatocellular carcinoma (HCC). EZH2 is a potent histone methyltransferase of H3K27me3, which has been determined as an oncogene in many malignancies. The CDKN2A-2B gene cluster encodes three important tumor suppressors, P14, P15 and P16. In this study, the anticancer effect and molecular mechanism of G-Rh2 on HCC was investigated. Treatment of HCC cells with G-Rh2 inhibited cell proliferation, migration and induced cell cycle arrest at the G0/G1 phase. We demonstrate for the first time that this effect was specifically mediated by down-regulating expression of EZH2. Further molecular mechanism study indicated that the decreased EZH2 promoted P14, P15 and P16 gene transcription through reducing H3K27me3 modification in the promoter of CDKN2A-2B gene cluster loci. Similarly, silencing of EZH2 by siRNA down-regulated P14, P15, P16 mRNA levels and inhibited HCC cell proliferation. Our results suggested that EZH2 could be a potentially therapeutic target by G-Rh2 in HCC, which provided a rationale for the development of drugs that inhibited histone methylase as a strategy against various cancers. | |||
| TO cite this article:Li Qi. Ginsenoside-Rh2 suppresses proliferation and migration of hepatocellular carcinoma cells by targeting EZH2 to regulate CDKN2A-2B gene cluster transcription[OL].[25 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4727383 | |||
| 7. The Effects of Colonic Inner Environment on Microbial Fuel Cell Performance | |||
| FU Yuming,Li Hongyan | |||
| Basic Medicine 22 April 2017 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (4K B) | |||
| Abstract:Microbial fuel cells (MFCs) can produce electricity by utilizing the microorganisms and organic compounds in human large intestine, which might provide a new way for implantable medical devices (IMDs) to have a lasting, stable and safe power source. Our previous work showed that MFC could power IMDs under colonic inner environment of healthy people, but the effects of environmental changes caused by diseases on electricity generation of MFC remain unclear. Therefore, five continuous-flow double-chamber MFCs with simulated colonic content as anodic substrate were constructed and the performance changes under different intestinal peristalsis rates, colonic temperature and pH values were investigated accordingly in this study. The results indicated that most of the environmental changes which may happen during illnesses could lead to the decrease of electricity generation of MFC, except the increase of colonic temperature. However, all of the MFCs could still generate electricity of at least 1.71 mW which could fulfil the requirement of most IMDs. The performance of MFC with multi-factor environmental change simulating diarrhea and fever could maintain stable for about 4h, but with prolonged disease duration, the output voltage dropped dramatically. Generally, MFC has strong potential to be used as power source for IMDs, even under most ill conditions, but strategies should still be applied to improve the stability of output to avoid some extreme conditions. | |||
| TO cite this article:FU Yuming,Li Hongyan. The Effects of Colonic Inner Environment on Microbial Fuel Cell Performance[OL].[22 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4728103 | |||
| 8. Nuclear translocation of DJ-1 protects the adult neuronal stem cells in MPTP-induced mouse Parkinson's disease model | |||
| SUN Yi,WANG Yupeng,LIN Yuanbin,ZHAO Xin,PU Xiaoping | |||
| Basic Medicine 21 April 2017 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (4K B) | |||
| Abstract:The DJ-1 gene, originally identified as an oncogene, is a causative gene for a familial form of Parkinson's disease (PD), PARK7. DJ-1 protein was found to play multiple roles in various physiological and pathological processes including cellular transformation, regulating oxidative stress and transcription, as well as cell growth and death. We found previously that DJ-1 was closely associated with the proliferation and differentiation of cultured neuronal stem cells. In this study, we further investigated other functions of DJ-1, in an attempt to determine how adult neurogenesis was affected in the subgranular zones (SGZ), the subventricular zone (SVZ) and olfactory bulb (OB), the brain areas closely related to early non-motor symptoms of PD, using an MPTP-induced mouse PD model. The results demonstrated that the number of BrdU+ cells in SGZ for both MPTP-treated and saline control groups were not significantly different. However, increased migration of BrdU+ cells into the olfactory glomerulus layer of OB was observed 14 days after MPTP injection, suggesting a compensatory mechanism might exist to restore neuronal loss. Specifically, we found that DJ-1 was translocated into the nuclei of the BrdU+ cells in SGZ and SVZ, but not in OB. A plausible explanation is that the nuclear translocation of DJ-1 mainly occurs in the early stages of adult NSCs proliferation in vivo. However, within the BrdU- cells in the olfactory glomerulus layer of OB, DJ-1 was concentrated into nuclei. These results indicated that the nuclear translocation of DJ-1 might be involved in maintaining the olfactory function of mature olfactory neurons. The research presented here thus provided evidence on using DJ-1 as a tool and biomarker for PD early diagnosis, stratification and possibly therapeutic treatments. | |||
| TO cite this article:SUN Yi,WANG Yupeng,LIN Yuanbin, et al. Nuclear translocation of DJ-1 protects the adult neuronal stem cells in MPTP-induced mouse Parkinson's disease model[OL].[21 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4725748 | |||
| 9. Seroprevalence of Hepatitis E among Pregnant Women in Zhenjiang, China | |||
| Rui Zhilian,Xiaochun Wang,Yang Yan,Peng Ying,Zhao Xiaoying,Wen Zhang,Fu Xingli,Zhou Chenglin,Yang Shixing,Shen Quan | |||
Basic Medicine 01 April 2017
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (4K B) | |||
| Abstract: Backgroud: HEV is the most common cause of acute viral hepatitis in the worldwide. Most of HEV infections are mild or subclinical, nevertheless, hepatitis E (HE) is characteristically associated with a high occurrence of symptomatic presentations of clinical syndrome in pregnant women. Objectives: So far, only few cases of HE during pregnancy have been reported, and the data of seroprevalence among pregnant women was extremely limited. The aim of this study is to assess the HEV prevalence in the pregnant women in Zhengjiang, China. Materials and Methods: A total of 225 serum samples taken from pregnant women were subjected to detection of anti-HEV. IgM positive samples were tested for HEV RNA by using reverse transcription-nested PCR method. Positive PCR products were sequenced and phylogenetically analyzed. Results: The prevalence of anti-HEV IgG and IgM in pregnant women were 21.8% (49/225) and 3.6% (8/225), respectively. Four of the eight IgM positive samples were positive to HEV RNA. Phylogenetic analyses indicated that the 4 HEV strains had distinct nucleotide sequence and were divided into two different subgenotypes in genotype 4. Conclusions: The prevalences in current study for IgG and IgM were higher than those in the other regions of China. Additional, all of the four strains belonging to genotype 4 suggested that the genotype 4 was the predominant genotype in this area. | |||
| TO cite this article:Rui Zhilian,Xiaochun Wang,Yang Yan, et al. Seroprevalence of Hepatitis E among Pregnant Women in Zhenjiang, China[OL].[ 1 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4723385 | |||
| 10. Fetal exposure to angiotensin II type 1 autoantibody induces hepatic insulin resistance in the adolescent offspring of rats | |||
| WEI Mingming,ZHANG Suli,YANG Xiaoli,WANG Li,ZHAO Chengrui,LEI Jinghui,WANG Pengli,LIU Huirong | |||
| Basic Medicine 09 February 2017
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| Show/Hide Abstract | Cite this paper︱Full-text: PDF (4K B) | |||
| Abstract:Fetal origin of adult disease has gained lots of attention in relation to the occurrence of insulin resistance. Studys found offspring of pregnant rats tested positive for angiotensin II type 1 receptor autoantibody (AT1-AA) exhibited both liver damage and systemic insulin resistance during adulthood. But the mechanism and time-course associated with symptom remain unclear. Normal pregnant rats were administered with preeclampsia serum-derived AT1-AA in the second trimester to establish AT1-AA positive pregnant rat models. Compared to saline group, fasting serum glucose and insulin levels, insulin resistance index values were higher, and impaired glucose tolerance, abnormal insulin tolerance, islet compensatory hypertrophy were observed in adolescent and middle-aged offspring of AT1-AA group. Triglyceride and systolic blood pressure levels were elevated in adolescence. Hepatic glycogen synthetase reduced in the third trimester, adolescence and middle age. Expression of insulin receptor subunit, insulin receptor substrate 1/2, and their phosphoprotein decreased in hepatic insulin signaling pathway of adolescent and middle-aged offspring of AT1-AA group. We found the offspring of AT1-AA positive pregnant rats exist insulin resistance in adolescence. Meanwhile, hepatic insulin receptor and downstream receptor pathway disorder may be an important mechanism of insulin resistance in AT1-AA positive pregnant rat offspring. | |||
| TO cite this article:WEI Mingming,ZHANG Suli,YANG Xiaoli, et al. Fetal exposure to angiotensin II type 1 autoantibody induces hepatic insulin resistance in the adolescent offspring of rats[OL].[ 9 February 2017] http://en.paper.edu.cn/en_releasepaper/content/4718781 | |||
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