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1. The total AvBD-6 gene expressed by Pichia pastoris | |||
Wenyu Sun,Anshan Shan,Qingquan Ma,Na Dong,Yanping Cao,Liang Wang,Zeyun Wang,Changjiang Yan | |||
Animal Husbandry, Veterinary Medicine 09 January 2013 | |||
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Abstract:Avian β-defenisin 6 (AvBD-6) is a 67 amino acid-long peptide that plays an important role in the innate immunity of chickens and is widely expressed in most tissues. In this study, the codon-optimized AvBD-6 gene was synthesized and cloned into the eukaryotic expression vector pPICZαA and expressed in Pichia pastoris. Expression levels of the recombinant AvBD-6 were tested by Tricine-SDS-PAGE, and the product concentration reached 213.0 mg/l after optimization of the expression conditions. Recombinant AvBD-6 exhibited antimicrobial activity against bacteria with the minimal inhibition concentration (MIC) from 5.8 to 46.6 μg/ml and showed no hemolytic activity. | |||
TO cite this article:Wenyu Sun,Anshan Shan,Qingquan Ma, et al. The total AvBD-6 gene expressed by Pichia pastoris[OL].[ 9 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4512514 |
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