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There are 7 papers published in subject: > since this site started. |
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1. Characterization of Akirin in the innate immune response of the kuruma shrimp Marsupenseus japonicus | |||
LIU Ning,SUN Jiejie,WANG Xianwei,WANG Lei,ZHANG Hongwei,ZHAO Xiaofan,WANG Jinxing | |||
Aquatic Science 27 November 2015 | |||
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Abstract:Akirin as a conserved nuclear protein functions in parallel with the NF-κB of the immune deficiency (IMD) pathway and the Toll-like receptor (TLR) pathway. In this study, the full-length cDNAs of Akirin and 14-3-3 in Marsupenaeus japonicus, named as MjAki and Mj14-3-3 were identified separately. MjAki was mainly expressed in hemocytes, heart and intestine and Mj14-3-3 was detected in all six tested tissues including hemocytes, heart, hepatopancreas, gills, stomach, and intestine. The expression of MjAki was upregulated in the hemocytes of shrimp challenged with Vibrio anguillarum and white spot syndrome virus (WSSV), Mj14-3-3 was first decreased and then increased in the shrimp challenged with V. anguillarum and no obviously change in the shrimp challenged with WSSV. The expression of some AMPs was obviously suppressed by knockdown of MjAki and Mj14-3-3 with RNA interference. The interaction of MjAki with Mj14-3-3, dorsal, relish was confirmed in vitro. We proposed that MjAki combined with Mj14-3-3, dorsal and relish, acted as an active nuclear factor of NF-κB dependent pathway in innate immunity of M. japonicus. | |||
TO cite this article:LIU Ning,SUN Jiejie,WANG Xianwei, et al. Characterization of Akirin in the innate immune response of the kuruma shrimp Marsupenseus japonicus[OL].[27 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4664578 |
2. Cloning, phylogeny and expression correlated to gonads development of Leptin genes in blunt snout bream | |||
ZHAO Honghao,ZENG Cong,YI Shaokui,WAN Shiming,Chen Baixiang,GAO Zexia | |||
Aquatic Science 14 November 2015 | |||
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Abstract:To investigate the leptin related genes expression patterns and their possible function during the gonadal development in fish, the cDNA and genomic sequences of leptin, leptin receptor (leptinR), and leptin receptor overlapping transcript like-1 (leprotl1) were cloned and their expression levels were quantified in the different gonadal development stages of Megalobrama amblycephala. The results showed that the full length cDNA sequences of leptin, leptinR, and leprotl1were 953, 3432 and 1676 bp, coding 168, 1,082, and 131 amino acid polypeptides, and the genomic sequences were 1836 bp, 28528 bp and 5480 bp, which respectively had 3, 15 and 4 exons, respectively. The phylogenetic analysis revealed that three genes were relatively conserved in fish species. Quantitative real-time PCR results showed that the three genes were ubiquitously expressed in all examined tissues during the different gonadal development stages. The leptin and leptinR took part in the onset of puberty, especially in female M. amblycephala, by increasing the expression levels in brain during the stage I to III of ovary. The expression levels of leptin and leptinR had significant differences between male and female in HPG axis tissues (P<0.05). The leptinR had the same variation tendency with leptin, but the opposite changes of expression levels were found in leprotl1, which may resist the expression of leptinR for inhibiting the function of leptin in target organ. These findings revealed details about the possible role of these genes in regulating gonadal maturation in fish species. | |||
TO cite this article:ZHAO Honghao,ZENG Cong,YI Shaokui, et al. Cloning, phylogeny and expression correlated to gonads development of Leptin genes in blunt snout bream[OL].[14 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4661884 |
3. miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle | |||
Yan Biao,Wang Zhenhua,Zhao Jinliang | |||
Aquatic Science 14 October 2014 | |||
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Abstract:MyoD is one of the helix-loop-helix proteins regulating muscle-specific gene expression in tilapia. Tight regulation of the MyoD protein level is necessary for the precise regulation of skeletal muscle development. MicroRNAs (miRNAs) are a class of regulatory RNAs that post-transcriptionally regulate gene expression. An increasing amount of evidence has suggested that miRNAs play an important role in regulating skeletal muscle development. We reasoned that MyoD expression may be regulated by miRNAs. Predictions from bioinformatics have identified a putative miR-203b target site in the 3′-UTR of the MyoD gene. Interestingly, miR-203b expression is negatively correlated with MyoD expression, whereas miR-203b suppression leads to a significant increase in MyoD expression, thereby activating MyoD downstream genes. A 3′-UTR luciferase reporter assay further verifies the direct interaction between miR-203b and MyoD. Taken together, our results reveal a novel molecular mechanism inwhich miRNA participates in transcriptional circuits that regulate gene expression in tilapia skeletal muscle.????? | |||
TO cite this article:Yan Biao,Wang Zhenhua,Zhao Jinliang. miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle[OL].[14 October 2014] http://en.paper.edu.cn/en_releasepaper/content/4613624 |
4. Dynamic expression pattern of Corticotropin releasing hormone and Urotensin I, II gene and responses to acute salinity and temperature challenge in zebrafish during early development | |||
Lei Luo,Aqin Chen,Jianghong Xiong,Congcong Hu,Weiqun Lu | |||
Aquatic Science 26 December 2013 | |||
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Abstract:Corticotropin releasing hormone (CRH), urotensin I (UI) and urotensin II (UII) are found throughout vertebrate species from fish to human. To further understand the role of CRH, UI and UII in teleosts during development, we investigated the expression pattern of CRH, UI, UIIα and UIIβ, and the response to acute salinity and temperature challenge during the early development of zebra?sh, Danio rerio. The results reveal that CRH, UI, UIIα and UIIβ mRNA are detected from 0hpf, and the relative expression levels alter along with development from 0hpf to 6dpf and reach the highest level at 6dpf except UIIα at 5dpf. After zebrafish at 2, 3, 4 and 5 dpf were exposed to an acute stress for 15min, no significant difference in expression levels of CRH, UI, UIIα and UIIβ mRNA was observed in response to acute osmotic (30ppt) stress compared with control, while cold stress (18℃) and hot stress (38℃) generally inhibited the mRNA levels of CRH, UI, UIIα and UIIβ at each time point apart from at 3dpf. These indicate that the contribution of CRH, UI, UIIα and UIIβ to stress response in zebrafish may be stressor-specific during early development. Overall, results from this study offer new insights into the developmental and temperature-specific function of CRH, UI, UIIα and UIIβ in zebrafish embryos and larvae. | |||
TO cite this article:Lei Luo,Aqin Chen,Jianghong Xiong, et al. Dynamic expression pattern of Corticotropin releasing hormone and Urotensin I, II gene and responses to acute salinity and temperature challenge in zebrafish during early development[OL].[26 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4578577 |
5. Daily rhythms of urotensin I and II gene expression and hormone secretion in the caudal neurosecretory system of the euryhaline flounder (Platichthys flesus) | |||
Lv Weiqun ,Ying Zhang,Jianghong Xiong,Richard Balment | |||
Aquatic Science 04 April 2013 | |||
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Abstract:The caudal neurosecretory system (CNSS) is a unique neuroendocrine structure for environmental adaptation in fish, and is the major site of expression and secretion of urotensin I (UI) and II (UII). This study examined daily changes in mRNA expression and the secretion profile of UI and UII in the CNSS. Daily rhythms were observed in mRNA level of CNSS UI, urophysis UI, plasma UII, glucose, potassium and sodium. No statistically significant (Cosinor, P>0.05) diel rhythmicity in mRNA level of CNSS UII, urophysis UII, cortisol, lactate, osmolality and chloride were detected. The calculated acrophase of sodium, cortisol, plasma UII, urophysis UII, urophysis UI and mRNA level of CNSS UI rhythms were recorded at 13:04 h, 13:39 h, 14:45 h, 15:27 h. 14:41 h and 14:39 h, respectively and a positive relationship was evident among them. The acrophase of glucose and potassium rhythms were recorded at 18:57 h and 22:35 h, respectively. The glucose levels increased progressively at the onset of the UII surge at 15:00 h and reached peak values at dusk. The results support the hypothesis that the CNSS may play a role in the control of co-ordinated daily changes in energy mobilization, nutritional behavior and osmoregulatory systems in euryhaline flounder. Our findings described for the first time the existence of daily rhythms of CNSS hormone expression and secretion in Platichthys flesus. These results reveal the importance of taking into account the time of day when assessing stress responses and evaluating UI and UII as physiological indicators of stress in this species. | |||
TO cite this article:Lv Weiqun ,Ying Zhang,Jianghong Xiong, et al. Daily rhythms of urotensin I and II gene expression and hormone secretion in the caudal neurosecretory system of the euryhaline flounder (Platichthys flesus)[OL].[ 4 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4532623 |
6. Comparative analysis of nuclear oligomerization domain proteins (NOD)-1 and NOD2 in orange-spotted grouper (Epinephelus coioides) | |||
HOU Qinghua,LU Danqi,YI Shibai,DING Xu,SUN Yan,ZHANG Huixian,LIU Xiaochun,ZHANG Yong,LIN Haoran | |||
Aquatic Science 25 December 2012 | |||
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Abstract:Nucleotide-binding oligomerization domain-containing proteins-1 and-2 (NOD1 and NOD2) are members of the NOD-like receptors (NLRs) family. They are both cytoplasmic receptors, and sense microbial infections/danger molecules to induce host innate immune response. In this study, the full-length ORF sequences of NOD1 and NOD2 were cloned, and the putative amino acid sequences were identi?ed in orange-spotted grouper (Epinephelus coioides). The complete open reading frame (ORF) of grouper NOD1 contained 2823 bp encoding a 940 amino acid protein. Grouper NOD2 cDNA contained a 2967 bp ORF, encoding a protein of 988 amino acid residues. Both grouper NOD1 and NOD2 had similar domains to human and fish counterparts. Phylogenetic tree analysis showed that grouper NOD1 clustered with grass carp, zebra?sh and channel cat?sh, while NOD2 was most closely related to fugu. Expression patterns of grouper NOD1 and NOD2 were next studied. NOD1 had the highest level of expression in skin while NOD2 in trunk kidney. Post Vibrio alginolyticus (strain EcGS020401), lipopolysaccharide (LPS) or PolyI:C challenges, gene expression of grouper NOD1 and NOD2 was stimulated to different extents. NOD1 showed a significant enhancement after LPS stimulation, but NOD2 increased more significantly after PolyI:C invasion, indicating that NOD1 and NOD2 may exert different effects on the eradication of bacteria and virus. The adaptor protein RIP-like-interacting CLARP kinase (RICK) and downstream molecule interleukin-8 (IL-8) were also induced at different levels after stimulation, which indicated that NOD1 and NOD2 signal transduction was involved in grouper innate immune protection against bacterial and viral infections. | |||
TO cite this article:HOU Qinghua,LU Danqi,YI Shibai, et al. Comparative analysis of nuclear oligomerization domain proteins (NOD)-1 and NOD2 in orange-spotted grouper (Epinephelus coioides)[OL].[25 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4499074 |
7. Expression Characteristics of vasa Gene at RNA and Protein Level in Apostichopus japonicus During Gametogenesis | |||
YAN Meng,SUI Juan,SHAO Mingyu,SHENG Wanqiang,ZHANG Zhifeng | |||
Aquatic Science 17 January 2012 | |||
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Abstract:The expression product of vasa gene is a widely conserved germ line marker and participates in the development regulation of reproductive system. However, no information on vasa gene in Holothuroidea is available. In this paper, we showed the expression characteristics of Apostichopus japonicus vasa gene (Aj-vasa) during gametogenesis in ovary and testis, using in situ hybridization and immunohistochemistry techniques. The expression of Aj-vasa RNA is strongest in early germ cells (oogoniums and spermatogonia), decreases as the maturation proceeded (oocyte and spermatocyte), and absents in spermatid and sperms. Our findings show that the overall expression trend of Aj-vasa RNA is similar with that of its protein(Aj-VASA) during gametogenesis. Nevertheless there was a sexuality difference about the subcellular location of vasa RNA and its protein which was reflected in the specifically expression in cytoplasm of female germ cells and the widely expression in both nucleus cytoplasm of male germ cells. It suggested that Aj-vasa RNA as well as Aj-VASA could be the marker of germ cells during gametogenesis. | |||
TO cite this article:YAN Meng,SUI Juan,SHAO Mingyu, et al. Expression Characteristics of vasa Gene at RNA and Protein Level in Apostichopus japonicus During Gametogenesis[OL].[17 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4462753 |
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