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1. Analysis of maize miRNA regulated by Exserohilum turcicum | |||
Fangli Wu,Weibo Jin | |||
Agronomy 25 January 2014 | |||
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Abstract:Northern leaf blight, caused by the fungus Exserohilum turcicum (Pass.), is a major disease of maize (Zea mays L.). MicroRNAs (miRNAs) have been recently reported as gene expression regulators related to several stress responses; however, evidence of the role of miRNAs in plant response to biotic stresses is limited. In this study, the miRNA expression patterns in maize in response to E. turcicum stress were investigated using a plant miRNA microarray platform. A total of 118 miRNAs were detected in mock- and E. turcicum-inoculated leaves. Among these miRNAs, miR530, miR811, miR829, and miR845 were identified as new miRNAs in maize through a homology-based approach. In addition, four miRNAs were differentially regulated in response to E. turcicum: miR811, miR829, miR845, and miR408. | |||
TO cite this article:Fangli Wu,Weibo Jin. Analysis of maize miRNA regulated by Exserohilum turcicum[OL].[25 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4583982 |
2. Dynamic Monitoring and Systemic Distribution of ‘Candidatus Liberibacter asiaticus’ in Affected Plant and Insect Vector by Taqman Real-Time PCR | |||
Zhou Binbin,Yin Youping,Hu Hao,Wang zhongkang | |||
Agronomy 27 May 2009 | |||
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Abstract:Huanglongbing(HLB) is one of the most destructive citrus diseases worldwide, in which the uncultured phloem-restricted α-proteobacterium ‘Candidatus Liberibacter’ is always associated. A Taqman real-time polymerase chain reaction (RTi-PCR) assay method specific for ‘Candidatus Liberibacter asiaticus’ (Las) has been developed previously. In this study, the RTi-PCR was used to examine and dynamic monitor the distribution of Las in the infected citrus plant and insect vector, citrus psyllid (Diaphorina citri). Quantitative analysis of Las titer indicated that it was distributed unevenly in plant and psyllid, and basically, those sections with more sieve-tube tissue had more pathogens. Dynamic analysis results showed that the titer has a significant change throughout a year in citrus leaf mid-vein and psyllid vector. Understanding the distribution and dynamic change of the bacterium inside the citrus tree and psyllid is critical for HLB research work like transmission mechanism and field epidemic regulation. The results in our study show that RTi-PCR is an excellent tool for quantitative diagnosis of HLB pathogen, as well as for monitoring the pathogen and assessing resistance and tolerance to Las in citrus breedings. Furthermore, the RTi-PCR is also an efficient technique to quantitatively evaluate the other endophytic bacteria in their host. | |||
TO cite this article:Zhou Binbin,Yin Youping,Hu Hao, et al. Dynamic Monitoring and Systemic Distribution of ‘Candidatus Liberibacter asiaticus’ in Affected Plant and Insect Vector by Taqman Real-Time PCR[OL].[27 May 2009] http://en.paper.edu.cn/en_releasepaper/content/32639 |
3. A multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of citrus huanglongbing and citrus bacterial canker disease | |||
Wang zhongkang,Zhou Binbin,Hu Hao,Yin Youping | |||
Agronomy 27 May 2009 | |||
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Abstract: | |||
TO cite this article:Wang zhongkang,Zhou Binbin,Hu Hao, et al. A multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of citrus huanglongbing and citrus bacterial canker disease[OL].[27 May 2009] http://en.paper.edu.cn/en_releasepaper/content/32626 |
4. Genetic diversity of “Candidatus Liberibacter asiaticus” isolates from different geographical regions in China | |||
Zhou Binbin,Yin Youping,Hu Hao,Wang Zhongkang | |||
Agronomy 26 May 2009 | |||
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Abstract:Citrus Huanglongbing (HLB) is one of the most destructive diseases. HLB was demonstrated grafting transmissibility via infected citrus scion in Southern China, therefore, it is important to investigate HLB disease epidemiology. In this study, a genetic diversity study was conducted using PCR-RFLP based on the outer membrane protein gene (omp) sequences. The amplicons of “Ca. L. asiaticus” were digested with enzymes. The difference of restriction patterns show that “Ca. L. asiaticus” may comprise several different omp strains, even among strains within a given region. DNA sequences and the deduced amino acid sequences of omp gene were compared to those in GenBank database with BLAST and the results were used to construct phylogenetic tree. The omp gene of “Ca. L. asiaticus” from China were highly homogeneous, but other “Candidatus. Liberibacter” species were not introduced or developed in china so far. The result is important on explorer HLB disease epidemiology and can help the scientific community develop more efficient methods to control the diseases. | |||
TO cite this article:Zhou Binbin,Yin Youping,Hu Hao, et al. Genetic diversity of “Candidatus Liberibacter asiaticus” isolates from different geographical regions in China[OL].[26 May 2009] http://en.paper.edu.cn/en_releasepaper/content/32562 |
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