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1. Network-based analysis and experimental validation explored Bu-Yang-Huan-Wu-Tang protects endothelial cells after hypoxia-reoxygenation injury by inhibiting the PI3K/AKT/FOXO1 pathway | |||
Shi Yan,Zhou Yue,Guan Qing,Wen Qingsi,Sun Yu,Yan Zewen,Li Yuye,Zhu Qingnan,Lin Hongli,Wang Dapeng | |||
Traditional Chinese Medicine and Pharmacology 01 March 2024 | |||
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Abstract:Purpose: To explore the drug targets and potential mechanisms of Bu-Yang-Huan-Wu-Tang (BYHWT) in the treatment of acute kidney injury (AKI) through network pharmacology and experimental validation. Methods: A network-based analysis research approach was used to predict the main active ingredients, targets of action and potential signaling pathways of BYHWT for AKI treatment. The protective effect of BYHWT on human umbilical vein endothelial cells (HUVEC) was verified by detecting the PI3K/AKT/FOXO1 signaling pathway and related factors through in vitro experiments. Results:A total of 124 active components of BYHWT were screened, with 444 action targets, 1,708 AKI disease-related target genes, and 210 targets at the intersection of components and diseases were obtained. Through GO and KEGG enrichment analysis, we obtained 219 signaling pathways of BYHWT for the treatment of AKI, and screened the top 10 pathways with the highest degree of enrichment, which involved the PI3K-AKT signaling pathway, and the experimental validation results showed that BYHWT-containing serum increased the expression of p-PI3K, p-AKT, and p-FOXO1, and increased the phosphorylation of FOXO1, and decreased the expression of FOXO1 expression. Conclusion: BYHWT may play a role in ameliorating endothelial cell apoptosis and protecting injured endothelial cells by regulating the PI3K/AKT/FOXO1 pathway. | |||
TO cite this article:Shi Yan,Zhou Yue,Guan Qing, et al. Network-based analysis and experimental validation explored Bu-Yang-Huan-Wu-Tang protects endothelial cells after hypoxia-reoxygenation injury by inhibiting the PI3K/AKT/FOXO1 pathway[OL].[ 1 March 2024] http://en.paper.edu.cn/en_releasepaper/content/4762351 |
2. Duhuo Jisheng decoction inhibits endoplasmic reticulum stress in chondrocytes induced by tunicamycin through the downregulation of miR-34a | |||
LIU Fayuan,WENG Xiaping,LIN Pingdong,ZHENG Chunsong,XU Huifeng,LIU Xianxiang,YE Hongzhi,LI Xihai | |||
Traditional Chinese Medicine and Pharmacology 10 September 2015 | |||
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Abstract:Our previous study showed that Duhuo Jisheng decoction (DHJSD) inhibited chondrocyte apoptosis by the mitochondria-dependent signaling pathway. Endoplasmic reticulum (ER) stress is upstream of the mitochondria-dependent signaling pathway and has been shown to promote chondrocyte apoptosis that occurs in osteoarthritis (OA). The present study aimed to evaluate whether DHJSD inhibits the chondrocyte apoptosis by regulating ER stress. DHJSD enhanced the viability of tunicamycin (TM)-exposed chondrocytes, a model of ER stress-induced apoptosis, in a dose- and time-dependent manner, as shown by MTT assay. The present results showed that DHJSD and sodium 4-phenylbutyrate (PBA), an ER stress inhibitor, reduced TM-induced chondrocyte apoptosis by 4',6-diamidino-2-phenylindole staining. To gain insight into the mechanisms of DHJSD that are responsible for enhancing the viability and inhibiting TM-induced chondrocyte apoptosis, the associated mRNA expressions and protein levels were detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis, respectively. The results showed that the expression levels of Xbp1, Xbp1s and Bcl-2 were increased, and the expression levels of Bip, Atf4, Chop, Bax, caspase-9 and -3 were decreased in the TM-exposed chondrocytes treated with DHJSD or PBA compared with that in the TM-exposed chondrocytes. To identify the possible mechanisms, the expression of miR-34a was examined by the TaqMan microRNA assay, and was downregulated in the TM-exposed chondrocytes treated with DHJSD or PBA compared with that in the TM-exposed chondrocytes. DHJSD inhibits ER stress in chondrocytes induced by exposure to TM by downregulating miR-34a, suggesting that DHJSD may be a potential therapeutic agent for OA. | |||
TO cite this article:LIU Fayuan,WENG Xiaping,LIN Pingdong, et al. Duhuo Jisheng decoction inhibits endoplasmic reticulum stress in chondrocytes induced by tunicamycin through the downregulation of miR-34a[OL].[10 September 2015] http://en.paper.edu.cn/en_releasepaper/content/4654175 |
3. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through inhibition of tau phosphorylation | |||
Song Yixiang,Miao Junye,Qiang Min,He Rongqiao,Wang Xuemei,Li Weiwei | |||
Traditional Chinese Medicine and Pharmacology 20 March 2014 | |||
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Abstract:BACKGROUND: Formaldehyde-induced neurotoxicity is implicated in the pathology of Alzheimer's disease. Icariin, a flavonoid found in Chinese herbal medicine Epimedium, exhibits neuroprotective activity; however, whether Icariin antagonizes formaldehyde-induced nerve injury is unclear. AIMS: To investigate the neuroprotective effects of Icariin on formaldehyde-treated SH-SY5Y cells and the possible mechanisms involved. METHODS: SH-SY5Y cells were divided into control group, formaldehyde treatment group, and Icariin treatment group. Cell viability, apoptosis, and morphological changes were determined by CCK8, flow cytometry, and confocal microscopy, respectively. The phosphorylation of protein tau was examined by Western blot. RESULTS: Formaldehyde showed a half lethal dose (LD50) of 0.3 mM in SH-SY5Y cells. Icariin (1 - 10 μM) prevented formaldehyde-induced cell death in SH-SY5Y cells in a dose-dependent manner, with the optimal effect observed at 5 μM. Examination of cell morphology by confocal microscopy demonstrated that 5 μM ICA significantly attenuated formaldehyde-induced cell injury. Additionally, Icariin inhibited formaldehyde-induced cell apoptosis in SH-SY5Y cells. Results from western blot analysis showed that Icariin suppressed formaldehyde-induced tau phosphorylation at Thr181 and Ser396, while having no effect on the total tau protein level. Furthermore, Icariin reduced Tyr216 phosphorylation and increased Ser9 phosphorylation of the tau kinase GSK-3β. CONCLUTION: Icariin protects SH-SY5Y cells from formaldehyde-induced injury possibly through inhibition of GSK-3β-mediated tau phosphorylation.????? | |||
TO cite this article:Song Yixiang,Miao Junye,Qiang Min, et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through inhibition of tau phosphorylation[OL].[20 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4590573 |
4. CDH1 gene polymorphisms and risk of gastric cancer associated with tongue coating appearance in a chinese population | |||
ZHAN Zhen,WU Juan,ZHANG Junfeng,YANG Yaping,TONG Shujuan,ZHANG Chunbing,LI Jin,YANG Xuewen,DONG Wei | |||
Traditional Chinese Medicine and Pharmacology 15 November 2011 | |||
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Abstract:Objective: Tongue coating appearances have always thought to be related with the progress of gastric caner in Chinese medicine. The genetic polymorphisms in E-cadherin gene (CDH1) may affect invasive/metastatic development of gastric cancer by altering gene transcriptional activity of epithelial cell. The main concern of this paper was to explore the associations among CDH1 gene polymorphisms, tongue coating appearance and predisposition of gastric cancer. Methods: Four potentially functional polymorphisms (rs13689, rs1801552, rs16260 and rs17690554) of the CDH1 gene were genotyped in a case-control study of 387 incident gastric cancer cases and 392 healthy controls by polymerase chain reaction-ligation detection reaction (PCR-LDR) method and the information of tongue coating was also collected. In order to simplify the tongue diagnosis for easily understood by biomedical professionals, only two typical tongue coating color (white and yellow) and three tongue coating amount (thick, thin and none) were identified for this analysis. Results: None of the four polymorphisms or their haplotypes achieved significant difference in their distribution between gastric cancer cases and controls. Multiple logistic regression analyses revealed that gastric cancer risk was not significantly associated with the variant genotypes of the four CDH1 polymorphisms as compared with their wild-type genotypes. However, combined the analyses of tongue coating appearance, we found, interestingly, that carrying C allele of rs13689 and G allele of rs17690554 could increase the risk of gastric cancer (OR=2.576, 95%CI=1.227-5.406 for rs13689; OR=2.154, 95%CI=1.014-4.574 for rs17690554, respectively) in individuals with yellow tongue coating. Conclusions: Our results indicate, for the first time, that genetic variants at CDH1 gene may play a role in gastric cancer preposition in consideration of specific tongue appearance in China. | |||
TO cite this article:ZHAN Zhen,WU Juan,ZHANG Junfeng, et al. CDH1 gene polymorphisms and risk of gastric cancer associated with tongue coating appearance in a chinese population[OL].[15 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4450077 |
5. Carboxymethylpachymaran Enhance Immunologic Function of Dendritic Cells Cultured In Two Kinds of Hepatoma Carcinoma Cell Line's Supernatant Related to NF-κB/Rel Pathway* | |||
YU Bin,CHEN Zhuo,WU Xian-lin,DAI Cong-qi,QIAN Guo-qiang,YU Jian-zhong,HE Hai-bin,WANG Zhi-xin,HOU Jun,CHEN Xiao-yin | |||
Traditional Chinese Medicine and Pharmacology 29 October 2011 | |||
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Abstract:Objective: To study the immunologic function of dendritic cells cultured in two kinds of hepatoma cell line's supernatant, and the enhancement effects of carboxymethylpachymaran (CMP) on dendritic cells (DCs). Methods: DCs were harvest after stimulated by granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) from umbilical cord blood using density gradient centrifugation method. Cultured supernatant of two hepatoma cell lines (HepG2 and HepG2.2.15) were collected for condition medium (CM) according to a volume ratio of supernatant to un-complete RPMI-1640 which was 3:1. CMP was dissolved in un-complete RPMI-1640 medium. Experimental groups were divided according to the culture medium, eithor condition medium or with CMP in it. DCs subsets cluster of differentiation 83 (CD83), CD86, CD1a and d-related human leukocyte antigens (HLA-DR) were analyzed by flow cytometry. The proliferation ability of allogeneic T cells in mixed lymphocyte reaction stimulated by DCs was examined by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) analysis. IL-12p70, interferon γ (IFN-γ) and nuclear factor kappa B (NF-κB) were detected by enzyme-linked immunosorbent assay (ELISA) analysis. Results: The proliferation of lymphocytes and secreting level of IL-12 and expression of phenotype of DCs cultured in two kinds of condition medium were lower than those of normal group (P<0.01). Compared with normal group, groups treated with CMP showed a higher expression level of DCs subsets, lymphocytes reproductive activity, as well as IL-12 and IFN-γ secretion levels. Groups treated with CMP also demonstrated higher levels of DCs phenotype expression, and IL-12 and IFN-γ secretion in supernatant of mixed lymphocyte reaction (MLR) and hgher lymphocytes reproductive activity as compared with CM group (P<0.05). Compared with the normal group, the expression level of NF-κB in DCs nuclear was higher in CMP groups, but lower in two CM groups (P<0.05). After CMP was added, the NF- κB expression levels of two CM groups were increased compared to those before adding (P<0.05). However, there was no significant difference between two CM groups (P>0.05). Conclusion: Two kinds of hepatoma cell line's supernatant can inhibit the immunologic function of DCs. And this suppressive effect may be related to the inhibition of NF-κB/Rel pathway. CMP may up-regulate the DCs function by activating the NF-κB/Rel pathway. | |||
TO cite this article:YU Bin,CHEN Zhuo,WU Xian-lin, et al. Carboxymethylpachymaran Enhance Immunologic Function of Dendritic Cells Cultured In Two Kinds of Hepatoma Carcinoma Cell Line's Supernatant Related to NF-κB/Rel Pathway*[J]. |
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