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| There are 11 papers published in subject: > since this site started. | |||
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| 1. Characterization of HIV-1 Variants Resistant to an Electronically Constrained Peptide Fusion Inhibitor | |||
| Xiyuan Wu | |||
| Basic Medicine 04 May 2017 | |||
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| Abstract:Emergence of drug resistant HIV-1 mutants is a serious problem for AIDS treatment. SC29EK, a shorter variant of SC34EK, is one of the representative next-generation fusion inhibitors of HIV-1. It shows high activity on inhibiting both wide type and T-20 resistant HIV-1 strains. In this study, we focused on the selection and characterization of HIV-1 escape mutants of SC29EK. Three mutations, Q39R, N43K and E49A located at N-heptad repeat regions and N126K located at C-heptad repeat region of gp41 were identified. High resistance to pocket targeting inhibitor peptides and cross-resistance to enfuvirtide (T20) and sifuvirtide (SFT) were detected, with the mechanisms of significantly reducing binding affinity of the inhibitors and dramatically enhancing interaction of the viral six-helix bundle. Our data helps increase the understanding of the structure-function relationship of gp41 and HIV-1 evolution, providing a reference for the clinical application of fusion inhibitors.????? | |||
| TO cite this article:Xiyuan Wu. Characterization of HIV-1 Variants Resistant to an Electronically Constrained Peptide Fusion Inhibitor[OL].[ 4 May 2017] http://en.paper.edu.cn/en_releasepaper/content/4732446 | |||
| 2. Seroprevalence of Hepatitis E among Pregnant Women in Zhenjiang, China | |||
| Rui Zhilian,Xiaochun Wang,Yang Yan,Peng Ying,Zhao Xiaoying,Wen Zhang,Fu Xingli,Zhou Chenglin,Yang Shixing,Shen Quan | |||
Basic Medicine 01 April 2017
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| Abstract: Backgroud: HEV is the most common cause of acute viral hepatitis in the worldwide. Most of HEV infections are mild or subclinical, nevertheless, hepatitis E (HE) is characteristically associated with a high occurrence of symptomatic presentations of clinical syndrome in pregnant women. Objectives: So far, only few cases of HE during pregnancy have been reported, and the data of seroprevalence among pregnant women was extremely limited. The aim of this study is to assess the HEV prevalence in the pregnant women in Zhengjiang, China. Materials and Methods: A total of 225 serum samples taken from pregnant women were subjected to detection of anti-HEV. IgM positive samples were tested for HEV RNA by using reverse transcription-nested PCR method. Positive PCR products were sequenced and phylogenetically analyzed. Results: The prevalence of anti-HEV IgG and IgM in pregnant women were 21.8% (49/225) and 3.6% (8/225), respectively. Four of the eight IgM positive samples were positive to HEV RNA. Phylogenetic analyses indicated that the 4 HEV strains had distinct nucleotide sequence and were divided into two different subgenotypes in genotype 4. Conclusions: The prevalences in current study for IgG and IgM were higher than those in the other regions of China. Additional, all of the four strains belonging to genotype 4 suggested that the genotype 4 was the predominant genotype in this area. | |||
| TO cite this article:Rui Zhilian,Xiaochun Wang,Yang Yan, et al. Seroprevalence of Hepatitis E among Pregnant Women in Zhenjiang, China[OL].[ 1 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4723385 | |||
| 3. Identification of the subtype diversity of genotype 4 hepatitis E virus in eastern China | |||
| ZHANG Wen,TIAN Hua,YANG Shixing,FU Xingli,LI Wang,HUANG Yuan,SUN Jiayao,ZHOU Gai,ZHOU Chenglin,SHEN Quan | |||
Basic Medicine 09 November 2014
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| Abstract:Hepatitis E viruses (HEVs), a zoonotic pathogen, use several species of animal as reservoirs. Swine is considered as the major reservoir for HEV infection in humans. Genotype 4 HEV is the dominant cause of hepatitis E disease in humans in China. Several recent studies showed that genotype 4 HEV is transmitted between humans and swine in China. In the present study, a total of 125 anti-HEV IgM positive human serum and 290 swine fecal samples were subjected to RT-PCR screening of HEV RNA. Results indicated 24 human serum samples were positive, while 9.66% (28/290) of the 290 swine fecal samples were positive for HEV RNA. Phylogenetic analysis based on partial capsid gene showed that the 51 HEV strains in the current study all belonged to genotype 4, clustering into 6 different subtypes. Some of HEV isolates prevalent in the human and swine populations were classified into the same clusters, suggesting that cross-species transmission occurred. Our study revealed the subgenotype diversity of genotype 4 HEV in eastern China. | |||
| TO cite this article:ZHANG Wen,TIAN Hua,YANG Shixing, et al. Identification of the subtype diversity of genotype 4 hepatitis E virus in eastern China[OL].[ 9 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4616844 | |||
| 4. Hepatitis B virus X protein increases the IL-1β-induced NF-kB activation via interaction with evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) | |||
| CHEN Wannan,LIU Lingling,JIAO Boyan,LIN Wansong,LIN Xinjian,LIN Xu | |||
| Basic Medicine 18 August 2014 | |||
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| Abstract:Hepatitis B virus X protein (HBx) transactivates multiple transcription factors including nuclear factor-kappa B (NF-κB) that regulates inflammatory-related genes. However, the regulatory mechanism of HBx in NF-κB activation remains largely unknown. This study reports that HBx augments the interleukin-1β (IL-1β)-induced NF-κB activation and interleukin-10 (IL-10) expression via interaction with a Toll-like receptor (TLR) adapter protein, ECSIT (evolutionarily conserved signaling intermediate in Toll pathways). GST pull-down and co-immunoprecipitation analyses showed that HBx directly interacted with ECSIT. Deletion analysis of HBx in a CytoTrap two-hybrid system revealed that the interaction region of HBx for ECSIT was attributed to aa 51-80. Co-transfection of HBx and ECSIT in IL-1β-stimulated cells appeared to activate IKK and IκB signaling pathway as phosphorylation of both IKK α/β and IκBα was increased whereas knockdown of ECSIT or HBx△51-80 mutant attenuated the phosphorylation. As a consequence of IκBα degradation, NF-kB was activated as evidenced by increases in NF-κB transcriptional activity and the nuclear translocation of p65 and p50 that resulted in the induction of IL-10. In contrast, knockdown of ECSIT by siRNA or treatment with an NF-κB selective inhibitor (helenalin) abolished the NF-κB activation and IL-10 expression. We conclude that ECSIT appears to be a novel HBx-interacting signal molecule and their interaction is mechanistically important in IL-1β induction of NF-κB activation. | |||
| TO cite this article:CHEN Wannan,LIU Lingling,JIAO Boyan, et al. Hepatitis B virus X protein increases the IL-1β-induced NF-kB activation via interaction with evolutionarily conserved signaling intermediate in Toll pathways (ECSIT)[OL].[18 August 2014] http://en.paper.edu.cn/en_releasepaper/content/4606851 | |||
| 5. Genome-wide screening of pathogenicity islands in Mycobacterium tuberculosis based on the genomic barcode visualization | |||
| WANG Guoqing,XU Guangyu,HU Jie,NI Zhaohui | |||
| Basic Medicine 03 July 2014
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| Abstract:Mycobacterium tuberculosis (M. tuberculosis) is one of the most widely spread human pathogenic bacteria, and it frequently exchanges pathogenesis genes among its strains or with other pathogenic microbes. The purpose of this study was to screen the pathogenicity islands (PAIs) in M. tuberculosis using the genomic barcode visualization technique and to characterize the functions of the detected PAIs. By visually screening the barcode image of the M. tuberculosis chromosomes, three candidate PAIs were detected as MPI-1, MPI-2 and MPI-3, among which MPI-2 and MPI-3 were known to harbor pathogenesis genes, and MPI-1 represents a novel candidate. Based on the functional annotations of Pfam domains and GO categories, both MPI-2 and MPI-3 carry genes encoding PE/PPE family proteins, MPI-2 encodes the type VII secretion system, and MPI-3 encodes genes for mycolic acid synthesis in the cell wall. Some of these genes were already widely used in early diagnosis or treatment of M. tuberculosis. The novel candidate PAI MPI-1 encodes CRISPR-Cas family proteins, which are known to be associated with persistent infection of M. tuberculosis. Our data represents a molecular basis and protocol for comprehensive annotating the pathogenic systems of M. tuberculosis, and will also facilitate the development of diagnosis and vaccination techniques of M. tuberculosis. | |||
| TO cite this article:WANG Guoqing,XU Guangyu,HU Jie, et al. Genome-wide screening of pathogenicity islands in Mycobacterium tuberculosis based on the genomic barcode visualization[OL].[ 3 July 2014] http://en.paper.edu.cn/en_releasepaper/content/4600909 | |||
| 6. L-Ficolin Binds to the Glycoproteins Hemagglutinin and Neuraminidase and Inhibits Influenza A Virus Infection Both in vitro and in vivo | |||
| PAN Qin,WANG Feng,CHEN Hadan,HOU Wei,Victor Tunje Jeza,ZHAO Yinglan,ZHANG Xiao-Lian | |||
| Basic Medicine 21 March 2013
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| Abstract:L-ficolin, one of the complement lectins found in human serum, is a novel pattern recognition molecule that can specifically bind to microbial carbohydrates, thereby activating the lectin complement pathway and mounting a protective innate immune response. However, little is known about the role of L-ficolin during viral infections in vivo. In the present study, we used a mouse model of influenza A virus infection to demonstrate that the administration of exogenous L-ficolin or ficolin A (FCNA - an L-ficolin-like molecule in the mouse) is protective against the virus. Furthermore, FCNA-null mice have a greatly increased susceptibility to infection with the influenza A virus. Moreover, we found recombinant human L-ficolin inhibited influenza A virus entry into Madin-Darby canine kidney cells. More importantly, L-ficolin can recognize and bind hemagglutinin (HA) and neuraminidase (NA) glycoproteins and different subtypes of influenza A virus,and these interactions can be competitively inhibited by N-acetyl-D-glucosamine. In addition, the binding of L-ficolin and FCNA may lead to the activation of the lectin complem e nt p athw ay. To o ur k n ow l e d g e, this is th e f ir s t re p o r t d e m onstrating that L-ficolin can bl ock influenza virus infections both in vitro and in vivo using FCNA-knockout mice, possibly by interacting with the carbohydrates of HA and NA. Therefore, these data may provide new immunotherapeutic strategies based on the innate immune molecule L-ficolin against the influenza A virus. | |||
| TO cite this article:PAN Qin,WANG Feng,CHEN Hadan, et al. L-Ficolin Binds to the Glycoproteins Hemagglutinin and Neuraminidase and Inhibits Influenza A Virus Infection Both in vitro and in vivo[OL].[21 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4531323 | |||
| 7. Comparison of direct boiling with commercial kits for extracting fecal microbiome DNA with Illumina sequencing of 16S rRNA tags | |||
| PENG Xin,ZHOU Hongwei,DENG Guanhua,JIANG Yunxia,WANG Yu,ZHANG Guoxia | |||
| Basic Medicine 16 January 2013
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| Abstract:High cost-efficiency and throughput are major advantages for determining metagenomic 16S rRNA tag sequences using next generation sequencing (NGS) techniques. These methods have significantly changed our view of microorganisms in fields of human health and environmental science. However, DNA extraction using commercial kits has become the major bottleneck because of its high cost and time consuming shortcomings. In the present study, we evaluated the determination of fecal microbiome using a direct boiling method compared to 5 different kinds of commercial methods, e.g. Qiagen and MO BIO kits. The PCoA analysis using UniFrac distances and the clustering result showed that direct boiling with a wide range of feces concentration had similar bacterial communities to most of the commercial kits, except the MO BIO method. The fecal concentration for boiling affected the estimation of α-diversity indices, but the results were generally comparable for boiling and commercial methods. The OTUs determined by direct boiling showed highly consistent frequencies with those determined by most of the commercial methods. Even for the MO BIO kit with an obviously different community structure, most of the OTUs determined at high to medium levels by the MO BIO kit were also determined in the direct boiling results with high confidences. Our present study suggested that direct boiling could be used to determine the fecal microbiome, and would significantly reduce the cost and improve the efficiency of the sample preparation for studying gut microbiome diversity.an) | |||
| TO cite this article:PENG Xin,ZHOU Hongwei,DENG Guanhua, et al. Comparison of direct boiling with commercial kits for extracting fecal microbiome DNA with Illumina sequencing of 16S rRNA tags[OL].[16 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4513274 | |||
| 8. Pseudomonas aeruginosa isolates of distinct sub-genotypes exhibit similar potential of antimicrobial resistance by drugs exposure | |||
| LIU Zhenhong,XU Yan,LIU Guirong,LIU Shulin | |||
| Basic Medicine 11 December 2012 | |||
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| Abstract:Pseudomonas aeruginosa, a wide-spread opportunistic pathogen, often complicates clinical treatments due to its resistance to a large variety of antimicrobials, especially in immune compromised patients, occasionally leading to death. However, the resistance to antimicrobials varies greatly among the P. aeruginosa isolates, which raises a question on whether some sub-lineages of P. aeruginosa might have greater potential to develop antimicrobial resistance than others. To explore this question, we divided 160 P. aeruginosa isolates collected from cities of USA and China into distinct genotypes using I-CeuI, a special endonuclease that had previously been proven to reveal phylogenetic relationships among bacteria reliably due to the highly conserved 26-bp recognition sequence. We resolved 10 genotypes by I-CeuI analysis and further divided them into 82 sub-genotypes by endonuclease cleavage with SpeI. Eight of the 10 genotypes contained both multi-drug resistant (MDR) and less resistant isolates based on comparisons of their antimicrobial resistance profiles (ARPs). When the less resistant or susceptible isolates from different genotypes were exposed to eight individual antimicrobials, they showed similar potential to become resistant with minor exceptions. This is to our knowledge the first report to examine correlations between phylogenetic sub-lineages of P. aeruginosa and their potential to become resistant to antimicrobials. This study further alerts the importance and urgency of antimicrobial abuse control. | |||
| TO cite this article:LIU Zhenhong,XU Yan,LIU Guirong, et al. Pseudomonas aeruginosa isolates of distinct sub-genotypes exhibit similar potential of antimicrobial resistance by drugs exposure[OL].[11 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4501720 | |||
| 9. Inhibitory Activity of Alltride, Sodium Houttuyfonate and Berberine Hydrochloride on Mycobacterium Tuberculosis | |||
| JIANG Xin,LU Chanyi | |||
| Basic Medicine 13 December 2010
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| Abstract:Three Chinese medicine monomers alltride, sodium houttuyfonate and berberine hydrochloride, has been identified some activity against tuberculosis in clinic application in China. To explore the activity and mechanism of them against Mycobacterium tuberculosis and multidrug-resistant Mycobacterium tuberculosis, MICs of them were performed in vitro. Comparison between the proteomes of Mycobacterium tuberculosis H37Rv, treated with and without alltride, differential protein spots were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MOLDI-TOF-MS). Alltride had the same inhibitory activity on susceptible and multidrug resistant Mycobacterium tuberculosis. Sodium houttuyfonate and berberine hydrochloride were higher inhibitory concentration on multidrug resistant Mycobacterium tuberculosis than susceptible. Four protein spots were downregulated in Mycobacterium tuberculosis H37Rv treated with alltride and identified as Rv2032，Rv0780，Rv1611 and Rv1284 by MOLDI-TOF-MS. The four proteins might be the targets of alltride. These findings suggest the multidrug resistance mechanisms of Mycobacterium tuberculosis are effective to berberine hydrochloride and sodium houttuyfonate. The anti-Mycobacterium tuberculosis mechanisms of these two drugs might be the similar with the one of the existing anti-tuberculosis drugs. Mechanism of alltride was different from them, might be inhibition biosynthesis of some nucleic acid and amino acid. | |||
| TO cite this article:JIANG Xin,LU Chanyi. Inhibitory Activity of Alltride, Sodium Houttuyfonate and Berberine Hydrochloride on Mycobacterium Tuberculosis[OL].[13 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4397485 | |||
| 10. Effects of uropathogenic Escherichia coli infection on gene expression profiles in human bladder transitional epithelial cells1 | |||
| Ge Xin,Dong jie,Chen jinying,Yao ping,Yang dongjing,Zhang yumei | |||
Basic Medicine 26 May 2009
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| Abstract:Interaction between uropathogenic Escherichia coli (UPEC) and host uroepithelial cells is the key step of the process of urinary tract infection. To further define the role uroepithelial cells play in initiating and modulating the host response to infection with UPEC strains, the human bladder transitional epithelial EJ cells were evaluated for their capacities to allow the adherence and invasion by UPEC132, a clinical strain isolated from China, and a cDNA microarray for 22 000 human genes was used to identify the gene expression differences between EJ cells infected with UPEC132 and uninfected EJ cells. Microscope observation showed that UPEC132 could adhere to EJ cells, and visualization by confocal microscope revealed that this invasive microorganism could be seen within the cells. EJ cells infected with UPEC132 significantly changed mRNA expression of a total of 29 genes, including 28 genes up-regulated and 1 gene down-regulated. Of these, regulators of growth and proliferation (e.g. immediate-early genes), cytokines (e.g. interleukin-6, interleukin-8), and modulators of apoptotic responses were the most prominent. In addition, the deduced signal transduction events based on bioinformatics analysis disclosed the complicated interaction between UPEC and host cells. | |||
| TO cite this article:Ge Xin,Dong jie,Chen jinying, et al. Effects of uropathogenic Escherichia coli infection on gene expression profiles in human bladder transitional epithelial cells1[OL].[26 May 2009] http://en.paper.edu.cn/en_releasepaper/content/32569 | |||
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