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1. Observe the difference of COVID-19 variation in different regions from the type and number of base mutations | |||
LIU Jian-Zhong, Zheng Jeffrey | |||
Biology 06 May 2020 | |||
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Abstract:Collecting Covid-19 genomes from three regions: Shanghai-China, Tbilisi-Georgia and Sydney-Australia, five similar genomes are selected from each region for research in this paper. Applying ”Datum gene sequence” method proposed,our results are shown that variation is immense in Sydney-Australia region, then variation is shown in the second in Tbilisi-Georgia region and it has a minimal value in Shanghai-China region respectively. | |||
TO cite this article:LIU Jian-Zhong, Zheng Jeffrey. Observe the difference of COVID-19 variation in different regions from the type and number of base mutations[OL].[ 6 May 2020] http://en.paper.edu.cn/en_releasepaper/content/4751960 |
2. Towards A new molecular tool for transgenic plants: Control of protein biosynthesis by a seed-specific promoter | |||
YANG Yuening,CHEN Moxian,ZHENG Shuxiao,YAO Yao,XU Chao,WANG Yin,LIU Jiesheng,CHYE Meelen,YANG Weidong,LI Hongye | |||
Biology 05 January 2013 | |||
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Abstract:[Background] Plants have been proven to be promising bioreactors in the production of many heterologous proteins including those in the biopharmaceutical industry. However, the efficiency of a potential plant bioreactor is impaired by the limitation in available promoters to drive foreign gene expression. Hence, the discovery of novel promoters is urgently needed. Seeds are ideal for protein storage and proteins can be kept as a stable form for several months. Thus a strong seed-specific promoter is a powerful tool in directing the expression and accumulation of target proteins in the seed. [Results] A 784-bp 5'-flanking sequence of the mung bean seed storage protein 8S globulin α' subunit gene was isolated by genome walking. When the sequence was analyzed with bioinformatics tools, numerous putative cis-elements were identified. The green fluorescent protein (GFP) reporter driven from this promoter was transiently expressed in protoplasts derived from mung bean cotyledons. Finally, transgenic Arabidopsis plants expressing the β-glucuronidase (GUS) reporter gene driven from the 8S globulin alpha' promoter were generated. GUS expression in transgenic seeds was observed by histochemical and quantitative GUS assays, confirming seed expression and high transcriptional activity. This study has identified the mung bean 8S α' promoter having potential for directing expression in seeds for bioreactor application. | |||
TO cite this article:YANG Yuening,CHEN Moxian,ZHENG Shuxiao, et al. Towards A new molecular tool for transgenic plants: Control of protein biosynthesis by a seed-specific promoter[OL].[ 5 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4510784 |
3. Improve on recovery of the recombinant human stem cell factor inclusion body in refolding with simultaneous purification process for large-scale | |||
Lili Wang,Chaozhan Wang ,Jiangfeng Liu,Xindu Geng | |||
Biology 06 March 2008 | |||
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Abstract: Recombinant expressed human stem cell factor (rhSCF) as cytoplasmic inclusion bodies (IB) are reported. In present work, to increase mass recovery of rhSCF production in large scale, the factors affecting about efficiency of rhSCF IB were recovered and solubilised in urea solution, and refolding with simultaneous purification process using protein folding liquid chromatography (PFLC) were investigated, including normal chromatographic column, the unit for the simultaneous renaturation and purification of proteins (USRPP). Finally, by combining the optimized buffer and USRPP, we were able to obtain 22 mg rhSCF with >95% purity.The mass recovery is 24 % for dilution, 38 % for the normal chromatographic column, 49 % for the USRPP. An average specific bioactivity is 4.27 ×105 IU/mg, 6.9×105 IU/mg, and 1.28×106 IU/mg, respectively. These protocol dates and new refolding with purification method -USRPP provide a cost effective and an efficient way to produce quantities of high purity rhSCF in large-scale. | |||
TO cite this article:Lili Wang,Chaozhan Wang ,Jiangfeng Liu, et al. Improve on recovery of the recombinant human stem cell factor inclusion body in refolding with simultaneous purification process for large-scale[OL].[ 6 March 2008] http://en.paper.edu.cn/en_releasepaper/content/19103 |
4. Functional characterization of a Root-knot nematode resistance gene Mi from tomato (Lycopersicon esculentum L.) | |||
Chen Rugang,Zhang Liying,Zhang Junhong,Zhang Wei,Wang Xue,Ouyang Bo,Li Hanxia,Ye Zhibiao | |||
Biology 29 March 2006 | |||
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Abstract:Root-knot nematodes (Meloidogyne spp.) cause major economic damage of numerous crop species around the world. Plant resistance is the most important attribute that is able to suppress invasion by the root-knot nematodes. In present study, a candidate root-knot nematode resistance gene Mi was isolated from the resistant tomato line RN-1. Expression profiling analysis revealed that this gene expressed specifically in roots, stems and leaves, but not in flowers or fruits. To verify the real function of this candidate gene, both the sense and RNAi vectors were constructed. We obtained thirty-one transgenic plants with 1 to 7 copies of T-DNA inserts of sense Mi from two nematode susceptible tomato cultivars as assayed by PCR and Southern blot analysis. RT-PCR analysis revealed that expression levels of Mi gene were varied in different transgenic plants. Nematode assays showed that the resistance to root-knot nematodes was significantly improved in some transgenic lines compared to untransformed susceptible control, and the resistance was inheritable in their selfed progenies. Loss of function via RNAi further confirmed the role of the Mi gene, and the original resistant lines became susceptible to root-knot nematodes. | |||
TO cite this article:Chen Rugang,Zhang Liying,Zhang Junhong, et al. Functional characterization of a Root-knot nematode resistance gene Mi from tomato (Lycopersicon esculentum L.)[OL].[29 March 2006] http://en.paper.edu.cn/en_releasepaper/content/5980 |
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