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1. 2,3,7,8-tetrachlorodibenzo-p-dioxin inhibits autophagy via Akt/ mTOR signaling pathway in RAW 264.7 macrophages | |||
Jing Lu,Meitong Liu,Haochen Zheng,Yong Fan,Qian Zhang,Shuang Guan | |||
Food Science and Technology 18 October 2020 | |||
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Abstract:2,3,7,8-tetrachlorodibenzodioxine (TCDD) was a highly toxic contamination and detected in the environment and food. It can be bio-magnified through food chain and bio-accumulate in human bodies, then cause various adverse health effects. Autophagy was a self-regulating process that degraded proteins and organelles in cells. The disruptions of autophagy balance have been bound up with multiple diseases and metabolic processes. In this paper, we detected the effects of TCDD on autophagy in RAW 264.7 cells and traced the molecular mechanism. The results showed that TCDD dose-dependently inhibited autophagy with the range from 0 to 10nM. Further, we found that this inhibition of autophagy was due to the decrease of p-Akt/Akt and increase of p-mTOR/mTOR expression. In addition, the autophagy inhibition caused by TCDD was resumed by using reversible mTOR inhibitor (rapamycin) and a selective Akt inhibitor (LY294002). This evidence showed that Akt/mTOR signaling pathway plays an important role in the autophagy induced by TCDD in RAW 264.7 cells. Our study confirmed the autophagy injury of TCDD on RAW 264.7 macrophages and the mechanism of TCDD toxicity was supplemented at the molecular level. | |||
TO cite this article:Jing Lu,Meitong Liu,Haochen Zheng, et al. 2,3,7,8-tetrachlorodibenzo-p-dioxin inhibits autophagy via Akt/ mTOR signaling pathway in RAW 264.7 macrophages[OL].[18 October 2020] http://en.paper.edu.cn/en_releasepaper/content/4752932 |
2. Comparative genomics and proteolytic enzymatic analysis of lactobacillus helveticus reveals strain biodiversity | |||
Qi Yanran,Liu Xiaoming | |||
Food Science and Technology 12 March 2019 | |||
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Abstract:Lactobacillus helveticus is a starter culture in the manufacture of Swiss cheese, Italian aged cheeses, and fermented milk products. Its growth relies on a complex proteolytic system that includes cell-envelope proteinases (CEPs) and peptidases. There were only a few strains of L. helveticus whose proteolytic systems in terms of genotype and phenotype have been characterized. In this study, a phylogenetic configuration and a pan/core genome analysis and an evaluation of the proteolytic enzymatic activities were conducted for 50 trains of L. helveticus including 38 isolated from various ecological niches in our lab. Three L. helveticus strains formed a phylogenetic lineage that was clearly separated from the other strains based on the phylogenetic tree, with the genome sizes from 1.798 to 2.255 Mbp. Diversity in key enzymatic activities was observed among L. helveticus strains. This study provides insight into the genetic and phenotypic diversity of L. helveticus isolated from various environments and the potential application in fermented dairy products. | |||
TO cite this article:Qi Yanran,Liu Xiaoming. Comparative genomics and proteolytic enzymatic analysis of lactobacillus helveticus reveals strain biodiversity[OL].[12 March 2019] http://en.paper.edu.cn/en_releasepaper/content/4747771 |
3. Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition | |||
Jiayu Gu,Bingyong Mao,Shumao Cui,Jianxin Zhao,Hao Zhang,Wei Chen | |||
Food Science and Technology 08 March 2018 | |||
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Abstract:Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut. Previous studies focused on the stimulated growth of Bifidobacterium, but did not further investigate the bifidobacterial composition, i.e. the specific species that was were stimulated. In this study mice were fed with FOS in different doses (0%, 5%, and 25%) for 4 weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3-V4 region and the groEL gene on the MiSeq platform, respectively. In the high-dose group (25% FOS) the relative abundance of Actinobacteria was significantly increased, which was mainly contributed by Bifidobacterium. At the genus level the relative abundances of Blautia and Coprococcus were also significantly increased. Through the groEL sequencing 14 species of Bifidobacterium were identified, among which Bifidobacterium pseudolongum (B. pseudolongum) was most abundant. After FOS treatment, B. pseudolongum became almost the sole bifidobacterial species (>95%). We isolated the B. pseudolongum strains and, by high performance liquid chromatography (HPLC), demonstrated their ability to metabolize FOS. Therefore, we concluded that FOS significantly stimulated the growth of B. pseudolongum in mice. Further investigations are needed to reveal the mechanism of selectiveness between FOS and B. pseudolongum, which would aid our understanding of the basic principles of dietary carbohydrates and host health. | |||
TO cite this article:Jiayu Gu,Bingyong Mao,Shumao Cui, et al. Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition[OL].[ 8 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4743709 |
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