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1. Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae | |||
LIU Jingying,YANG Xizhe,DONG Hui | |||
Biology 07 September 2015 | |||
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Abstract:The PCR RACE method was used to isolate LcSAP from Leymus chinensis. The 17.6 kDa LcSAP product comprises 161 residues. RT-PCR analysis showed that LcSAP was more strongly transcribed in the leaf than in the root, and that it was up-regulated by the imposition of salinity stress. When expressed heterologously in brewers' yeast, the gene enhanced the yeast cells' salinity tolerance. | |||
TO cite this article:LIU Jingying,YANG Xizhe,DONG Hui. Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae[OL].[ 7 September 2015] http://en.paper.edu.cn/en_releasepaper/content/4653399 |
2. Analyzation of relationship between soil nutrients and podophyllotoxin accumulation in Podophyllum hexandrum and Dysosma versipellis | |||
LI Mengfei,ZHANG Jing,LI Jinghua,ZHOU Lanlan,LI Tiantian,LI Wei | |||
Biology 06 March 2013 | |||
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Abstract:In order to analyze the correlation between soil nutrients and PDT accumulation. The experiment was designed to determine the PDT content in rhizomes of P. hexandrum and D. versipellis by high performance liquid chromatography (HPLC) and nutrients content in soil by high performance ion chromatography (HPIC) and high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS). The results of linear regress analysis showed that the PDT accumulation was positive relationship with NO3-, PO43-, Na+, Fe, Mn, and negative relationship with SO42-, K+, while Mg2+, Ca2+, Cu and Zn didn't reach significant levels. This study information can be helpful to produce PDT and related cyclolignans by commercial cultivation, cell and tissue cultures of P. hexandrum, D. versipellis and other related plant sources. | |||
TO cite this article:LI Mengfei,ZHANG Jing,LI Jinghua, et al. Analyzation of relationship between soil nutrients and podophyllotoxin accumulation in Podophyllum hexandrum and Dysosma versipellis[OL].[ 6 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4526883 |
3. In vivo localization of action sites of tenuazonic acid in photosynthetic apparatus by chlorophyll a fluorescence kinetics OJIP | |||
CHEN Shiguo,ZHANG Min,Strasser Reto J?rg,QIANG Sheng | |||
Biology 02 February 2012 | |||
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Abstract:Tenuazonic acid (TeA), a phytotoxin produced by the fungus Alternaria alternata isolated from diseased Crofton weed (Eupatorium adenophorum), exhibits a strong inhibition in photosynthesis, especially photosystem II (PSII) activity. In vivo the chlorophyll fluorescence induction transient of host plant and in vitro fluorescence transient of six kinds of other higher plants show that TeA acts several sites in photosynthesis apparatus. First, as the classical PSII inhibitors, the most important action site of TeA is that it interrupts electron transport beyond QA on the acceptor side of PSII due to binding to the QB-niche. Moreover, TeA leads to severe inactivation of PSII reaction centers (RCs). On the other hand, TeA has no affect on the antenna pigments, the energy transfer from antenna pigment molecules to RCs, and the oxygen-evolving complex (OEC) at the donor side of PSII. Nevertheless, it's different from the known classical PSII inhibitors that the dominant influence of TeA is not on the primary photochemical reaction but the biochemical reaction after QA. On the basis of the competition experiments with [14C]atrazine, it's further confirmed that TeA does not share with atrazine and DCMU the same binding environment despite their common action target: the QB-site. Additionally, TeA causes inactivation of the FNR system and therefore the reduction of NADP+ at PSI electron acceptor side. These actions distinguish TeA from the classical photosynthesis inhibitors. This provides perhaps a new idea and approach to help the search and development of bioherbicides in tetramic acid families or biomimetic synthesis of new herbicides from TeA precursor. | |||
TO cite this article:CHEN Shiguo,ZHANG Min,Strasser Reto J?rg, et al. In vivo localization of action sites of tenuazonic acid in photosynthetic apparatus by chlorophyll a fluorescence kinetics OJIP[OL].[ 2 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4464180 |
4. Expression of anthocyanin biosynthetic genes during fruit development in Fengxiang strawberry | |||
ZHOU Bo,LI Yuhua | |||
Biology 01 February 2012 | |||
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Abstract:Anthocyanins are the main pigments in flowers and fruits. In most cases, anthocyanin accumulation in fruit is highly controlled by the developmental level. In this study, the cDNA fragments of three genes, chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS), which are involved in the flavonoid pathway, were isolated from total RNA of strawberry ripe fruit by using polymerase chain reaction technique and labeled as probes to determine the expression of anthocyanin biosynthetic genes. Northern analysis showed that a correlation between anthocyanin accumulation and expression of the flavonoid pathway genes during the ripening of strawberry fruits. At the early stages of fruit development, the mRNA levels encoding CHS, DFR, ANS were high probably responsible for the accumulation of condensed tannins, but the levels decreased dramatically when fruits turned white from green. During the stage of pigment accumulation, their mRNA levels increased strongly to be involved anthocyanin biosynthesis. Difference of CHS in mRNA abundance was correlated with differential accumulation of anthocyanins throughout the process of fruit development. Therefore, CHS could be a key structure gene involved in anthocyanin synthesis. Furthermore, the co-ordination of expression of anthocyanin biosynthetic genes implied a common regulatory mechanism controlling the expression of structural genes in the flavonoid pathway. | |||
TO cite this article:ZHOU Bo,LI Yuhua. Expression of anthocyanin biosynthetic genes during fruit development in Fengxiang strawberry[OL].[ 1 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4464645 |
5. Changes in chloroplast ultrastructure, fatty acid components of thylakoid membrane and chlorophyll a fluorescence transient in flag leaves of a super-high-yield hybrid rice and its parents during the reproductive stage | |||
Mei-Ping Zhang,Guo-Xiang Chen | |||
Biology 14 January 2010 | |||
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Abstract:In plants, it is well established that chloroplast is one of the early targgeted organelles to break down during leaves senescing. Here we applied a newly-developed super-high-yield hybrid rice (Oryza sativa L.) LiangYouPeiJiu (LYPJ) and its parents lines to investigate changes in ultrastructure of chloroplasts, fatty acid composition of thylakoid membrane lipids and chlorophyll a fluorescence transient in natural senescing leaves. We found that at full expansion of flag leaves in three lines, chloroplasts often showed oblong shapes with a typical membrane system of stroma and grana thylakoids, whereas their shapes had been changed from oblong to spherical during senescence. Our data showed that the initiation of senescence displayed accumulation of starch and an increase in the number and size of plastoglobuli with the damaged thylakoid membranes; subsequently, swollen thylakoid membranes in stroma and in grana with a significant increase in MDA content, and disorganization of thylakoid membrane system with significant changes in fatty acid composition of thylakoid membrane lipids were developed. Compared with its parents, the newly-developed hybrid rice LYPJ had the highest capacity of carbohydrate transport from leaves (sources) to ears (sink), the lowest accumulation of starch grains in the leaf chloroplasts, and the slowest senescing rate of chloroplast in overall leaf senescence process. Chlorophyll a fluorescence transients of three kinds of flag leaves were analysed by so-called JIP-test. The results of analysis suggest that these findings inculding a high inherited activity of antioxidant enzymes and high photosynthetate transport to pretect chloroplast structure in the hybrid rice LYPJ have close relations to its super-high yield . | |||
TO cite this article:Mei-Ping Zhang,Guo-Xiang Chen. Changes in chloroplast ultrastructure, fatty acid components of thylakoid membrane and chlorophyll a fluorescence transient in flag leaves of a super-high-yield hybrid rice and its parents during the reproductive stage[OL].[14 January 2010] http://en.paper.edu.cn/en_releasepaper/content/38983 |
6. Sexual differences in photosynthetic characteristics of Ginkgo during leaf senescence | |||
Dawei Shi,Xiaodong Wei,Guoxiang Chen,Yanli Xu | |||
Biology 13 January 2010 | |||
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Abstract:Photosynthetic characteristics were compared between female and male ginkgoes, to investigate the differences in photosynthetic decline of leaves between two species during leaf senescence. Photosynthetic rate was used as a biomarker to define the initiation of leaf senescence which could be divided two stages. Therefore, the leaf senescence of ginkgo occurred at late August which could be the beginning of autumn. During the early stages of leaf senescence, photosynthetic functions, such as the electron transport activities of photosystems and photophosphorylation, and scavenging activities were lower in female than in male. But at the late senescence, these characteristics of female were higher those of male. With leaf aging, the damage to chloroplast of female induced by senescence was more than that of male. Our results found that there was a similar rate of leaf senescence between female and male, which may be the reason why the ginkgo has declined during the evolution of plant senescence. | |||
TO cite this article:Dawei Shi,Xiaodong Wei,Guoxiang Chen, et al. Sexual differences in photosynthetic characteristics of Ginkgo during leaf senescence[OL].[13 January 2010] http://en.paper.edu.cn/en_releasepaper/content/38873 |
7. Effects of low temperature on membrane lipid peroxidation in a newly developed super-high-yield hybrid rice (Oryza sativa L.) at seedling stage | |||
Dawei Shi,Xiaodong Wei,Guoxiang Chen,Chengjun Zhang,Ping Wang,Jing Wang,Chuangen Lv | |||
Biology 23 November 2009 | |||
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Abstract:To study the effects of low temperature on high-yield hybrid rice (Oryza sativa L.) at seedling stage, chlorophyll content, membrane lipid peroxidation, fatty acids composition and chloroplast ultrastructure of two varieties were investigated—a newly-developed cultivated super-high-yield hybrid rice LYPJ, and a traditional hybrid rice SY-63, setting 25℃ as control and 10℃ as low temperature treatment. In SY-63, chlorophyll content in rice leaves was decreased obviously with the duration at low temperature, whereas it did not decrease statistically in LYPJ. MDA (malondialdehyde) content increased slightly in LYPJ, but in SY-63, MDA content increased higher than those in 25℃-treated seedlings. The contents of saturated fatty acids decreased and the contents of unsaturated fatty acids increased in both of the two hybrids compared to the control. The IUFA (index of unsaturated fatty acid) of fatty acids increased in the two cultivars, being lower in LYPJ. The damage to the ultrastructure of chloroplasts was not so serious with increasing cold stress, but the number of osmiophilic granules in SY-63 increased significantly. The results indicated that LYPJ could have stronger chilling-resistance than SY-63 at seedling stage. | |||
TO cite this article:Dawei Shi,Xiaodong Wei,Guoxiang Chen, et al. Effects of low temperature on membrane lipid peroxidation in a newly developed super-high-yield hybrid rice (Oryza sativa L.) at seedling stage[OL].[23 November 2009] http://en.paper.edu.cn/en_releasepaper/content/36947 |
8. A Fibre-Assembling-Pressure Model for Developing Root Pressure | |||
Wang Xiaoen | |||
Biology 12 December 2008 | |||
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Abstract:For decades, it has well been known that the drive force of conducting both water and dissolved mineral ions from roots to leaves of plants makes mainly up of two: the pull of transpiration from leaves and root pressure. It had early been clear with regard to the transpiring pull, but mechanisms how root pressure be developed, hitherto, is fuzzy. Based on both experimental phenomenon of siphon-like transport of water defying gravity along fibre surface and crystallisation mechanisms, this paper theoretically suggests a fibre-assembling-pressure model for developing root pressure. According to the model, in roots the continuous polymerization of fiberized network, such as formation of nascent cell wall and maturity of xylem, can develop the fibre-assembling pressure, a positive hydraulic pressure. For a plant that grows with vigor, growth of all of fibres, such as xylem, can provide a drive force for water transport, division and expansion of cells in meristems, and gravitropic growth of roots. | |||
TO cite this article:Wang Xiaoen . A Fibre-Assembling-Pressure Model for Developing Root Pressure [OL].[12 December 2008] http://en.paper.edu.cn/en_releasepaper/content/26564 |
9. Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phrase High Performance Liquid Chromatography Under Salt and Alkali Stress | |||
Hong Yan | |||
Biology 20 September 2006 | |||
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Abstract:Several organic acids accumulated in Kochia Scoparia shoots and roots were studied by means of reverse-phrase high performance liquid chromatography with A C18 column. Five types of binary organic acids were separated. The organic acid concentrations were determined in K. Scoparia seedlings stressed by saline (NaCl) and alkaline (NaHCO3) at the same Na+ concentration. Concentrations of organic acids were stimulated by alkaline because the cells will adjust their pH values through the accumulation of organic acids, when the environment is basic. The concentrations of oxalic acid and succinic acid are higher than those of other organic acids, including tartaric acid and malic acid, and the concentration of citric acid is the lowest. The concentrations of organic acids in roots are higher than those in shoots under salt (NaCl) stress, but he results are opposite while the roots are under alkali (NaHCO3) stress. This indicates that there are different adaptive strategies for K. Scoparia seedlings in organic acid metabolism under salt and alkali stress | |||
TO cite this article:Hong Yan. Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phrase High Performance Liquid Chromatography Under Salt and Alkali Stress[OL].[20 September 2006] http://en.paper.edu.cn/en_releasepaper/content/8417 |
10. Identification of diurnal and circadian candidate genes in rice by using cDNA array | |||
Feng Zhang,Haitao Dong,Dean Jiang,Debao Li | |||
Biology 30 December 2005 | |||
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Abstract:cDNA array containing 5837 rice unique genes was used to explore gene expression patterns at 4 h intervals throughout a day. It was found that there were 190 genes with diurnal changes, of which 170 genes could change in circadian manner. Analysis of those genes showed that circadian clock played a key role in coordination of many physiological processes such as glycolysis, water and mineral elements transport. By clustering diurnal change genes from cDNA data, we found 10 clusters with different expression patterns. | |||
TO cite this article:Feng Zhang,Haitao Dong,Dean Jiang, et al. Identification of diurnal and circadian candidate genes in rice by using cDNA array[OL].[30 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4787 |
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