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1. Key Differential Gene Analysis between Ewing Sarcoma Cado-Es1 Cell Line Tumor Stem Cells, Non-Tumor Stem Cells, and Mesenchymal Stem Cells | |||
Hongliang Wu,Sihang Zheng,Qun He,Yan Li | |||
Clinical Medicine 24 March 2022 | |||
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Abstract:Background Ewing sarcoma is a highly malignant tumor. It is of great significance to identify new biomarkers or therapeutic targets. This study aimed to analyze the differentially expressed genes between Ewing sarcoma ERG type cell line cado-es1 tumor stem cell like cells, non-tumor stem cell like cells and mesenchymal stem cells (MSC) derived from Ewing sarcoma patients, and study the key genes related to Ewing sarcoma ERG type. Methods The NCBI SRA database data ERP104460 was retrieved, and three groups of MSC cells, tumor stem cell like cells (SP group) and non-tumor stem cell like cells (nsp group) obtained from different Ewing sarcoma patients were selected. The RNA-seq data of 9 samples in each group were analyzed to screen the significantly differentially expressed genes. Pathway enrichment analysis and the protein-protein interaction network was performed, and the hub genes were screened. Results Compared with MSC group, 8286 differentially expressed genes were obtained, including 3638 up-regulated genes and 4648 down-regulated genes. There were 9097 differentially expressed genes in nsp group compared with msc group, including 4800 up-regulated genes and 4297 down regulated genes. Compared with nsp group, four differentially expressed genes were obtained, including three up-regulated genes, U2, SNORA45b and RNU2-1. One down regulated gene was MTND1P27. These differentially expressed genes are mainly involved in biological processes and biological pathways such as cell division, cell cycle and DNA replication. By GO, KEGG and protein interaction network, 10 hub genes including THBS1, POSTN, COL1A1, COL1A2, MMP2, MMP14, SPARC, DCN, SPP1 and COL3A1 were screened in sp vs msc group; 10 hub genes including THBS1, MMP2, LOX, POSTN, COL1A1, COL1A2, MMP14, SPARC, DCN and SPP1 were obtained in nsp vs msc group. The key differential genes of PPI network between sp vs msc group and nsp vs msc group were COL3A1 and LOX. Conclusion THBS1, POSTN, COL1A1, COL1A2, MMP2, MMP14, SPARC, DCN, SPP1 and COL3A1 are key hub genes in Ewing sarcoma tumor stem cell like cells; THBS1, MMP2, LOX, POSTN, COL1A1, COL1A2, MMP14, SPARC, DCN and SPP1 are key hub genes in non-tumor stem cell like cells of Ewing sarcoma. | |||
TO cite this article:Hongliang Wu,Sihang Zheng,Qun He, et al. Key Differential Gene Analysis between Ewing Sarcoma Cado-Es1 Cell Line Tumor Stem Cells, Non-Tumor Stem Cells, and Mesenchymal Stem Cells[OL].[24 March 2022] http://en.paper.edu.cn/en_releasepaper/content/4757042 |
2. Treatment of primary pulmonary malignant fibrous histiocytoma: case report and literature Review | |||
XU Song,Li Xiongfei,SHI Tao,REN Fan,YANG Fan,Liu Renwang,REN Dian,DONG Shangwen,FAN Haiyang,WEI Sen,CHEN Gang,CHEN Jun | |||
Clinical Medicine 15 April 2017 | |||
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Abstract:Objective: Malignant fibrous histiocytoma (MFH) is an aggressive soft tissue sarcoma which can occur in various organs. However, primary MFH arising from lung is quite rare. Method: Herein we reported a case of 61-year-old male with primary pulmonary MFH and explore the underlying mechanisms by next generation sequencing. Results: Five gene mutations, including TSC2, ARID1B, CDK8, KDM5C and CASP8, were detected and the mTOR inhibitor might be an efficient treatment for this patient. In addition, we reviewed in the English literature approximately 36 case reports of primary pulmonary MFH since 1979 and summarized the clinical features and prognosis of this rare pulmonary malignant tumor.Conclusion:MFH is not sensitive to chemo- and radio-therapy. Once the diagnosis is confirmed, a complete surgical resection is necessary. Target therapy by mTOR inhibitors might be a promising treatment. | |||
TO cite this article:XU Song,Li Xiongfei,SHI Tao, et al. Treatment of primary pulmonary malignant fibrous histiocytoma: case report and literature Review[OL].[15 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4725565 |
3. Over-expression of LAPTM4B-35 and VEGF is related to poor prognosis in local advanced cervical carcinoma | |||
Fanling Meng,Pan Shang,Hongtao Song,Ge Lou | |||
Clinical Medicine 04 June 2016 | |||
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Abstract:Objectives: Patients with locally advanced cervical cancer (LACC) are difficult to treat. The purpose of this study is to investigate lysosome-associated protein transmembrane 4?-35 (LAPTM4B-35) and vascular endothelial growth factor (VEGF) expression in LACC and evaluate their clinicopathological and prognostic significance. Methods: The expression of LAPTM4B-35 and VEGF proteins was analyzed by immunohistochemistry in 119 LACC and 40 normal cervical specimens. Results: LAPTM4B-35 and VEGF were expressed in 73.9% and 75.6% of cervical cancer, respectively, which are higher than the normal cervical tissues. LAPTM4B-35 expression was positively correlated with the expression of VEGF. The expression of LAPTM4B-35 and VEGF was significantly correlated with FIGO stage, Histological grade and lymph node metastasis (P<0.05). Kaplan-Meier analysis showed that LACC patients with high expression of LAPTM4B-35 and VEGF exhibited poor overall survival (OS) and disease-free survival (DFS) (P<0.05). Conclusions: We demonstrate that LAPTM4B-35 correlates with VEGF and is a poor prognostic factor in local advanced cervical cancer. | |||
TO cite this article:Fanling Meng,Pan Shang,Hongtao Song, et al. Over-expression of LAPTM4B-35 and VEGF is related to poor prognosis in local advanced cervical carcinoma[OL].[ 4 June 2016] http://en.paper.edu.cn/en_releasepaper/content/4693517 |
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