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1. Sexual dimorphism of steroidogenesis regulated by GnIH in the Goldfish, Carassius auratus | |||
QI Xin,ZHOU Wenyi,LU Danqi,LI Shuisheng,LIU Xiaochun,ZHANG Yong,LIN Haoran | |||
Biology 22 December 2012 | |||
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Abstract:Gonadotrophin-inhibitory hormone (GnIH) has pronounced inhibitory effects on reproduction in a number of species. GnIH suppresses gonadotropin synthesis/release at the hypothalamic and pituitary levels; however, increasing evidences suggested a putative function on the gonad. In this study, we have demonstrated the localization of receptors for GnIH in ovary and testis of goldfish. In situ hybridization showed that goldfish GnIHRs were localized only in the oocytes before cortical alveolus stage, and were localized in the interstitial tissue of testis. Implantation of goldfish GnIH peptides reduced the serum estradiol level in the female goldfish, but enhanced serum testosterone level in the male goldfish. Besides, injection of goldfish GnIH peptides increased the expression of StAR and 3bHSD mRNA and decreased the expression of CYP19 mRNA significantly in testis, while showed no effect on these genes in ovary. In addition, in vitro results showed that goldfish GnIH peptides not only increased the expression of StAR, 3bHSD and decreased CYP19 mRNA, but also increased the expression of FSHR and LHR mRNA in testis cells, but showed no effect on the expression of detected genes in ovary cells. Thus, we believe that GnIH could contribute to the sexual dimorphism of steroidogenesis in goldfish. | |||
TO cite this article:QI Xin,ZHOU Wenyi,LU Danqi, et al. Sexual dimorphism of steroidogenesis regulated by GnIH in the Goldfish, Carassius auratus[J]. |
2. Evidences for the regulation of GnRH and GTH expression by GnIH in the goldfish, Carassius auratus | |||
QI Xin,ZHOU Wenyi,LI Shuisheng,LU Danqi,YI Shibai,XIE Rui,LIU Xiaochun,ZHANG Yong,LIN Haoran | |||
Biology 21 December 2012 | |||
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Abstract:Gonadotrophin-inhibitory hormone (GnIH) plays an important role in regulating of reproduction in teleosts. To clarify the mode of action of GnIH on the synthesis of gonadotropin releasing hormone (GnRH) and gonadotrophin (GtH), three GnIHR cDNAs were cloned from the goldfish brain. In situ hybridization results showed that GnIHRs were localized to the hypothalamus and pituitary. In the hypothalamus, GnIHRs were found in the NPP,NPO and NLT, whereas sGnRH neurons were reported to be located, and potentially regulated by GnIH. In the pituitary, only two GnIHRs were observed and they were localized to the PI instead of the adenohypophysis where GtH-expressing cells are localized, suggesting indirect regulation of GtH by GnIH. In vivo, intraperitoneal (i.p.) injections of synthetic goldfish GnIH-Ⅱ peptide and GnIH-Ⅲ peptide significantly decreased sGnRH and FSHβ mRNA levels. Only GnIH-Ⅱ decreased LHβ mRNA levels significantly. In vitro, both GnIH-Ⅱ and GnIH-Ⅲ showed no effect on GtH synthesis, but an inhibition of GnRH-stimulated LHβ and FSHβ synthesis was observed when GnIH-Ⅲ was applied to primary pituitary cells in culture. Thus, GnIH could contribute to the regulation of gonadotropin in the brain and pituitary in teleosts. | |||
TO cite this article:QI Xin,ZHOU Wenyi,LI Shuisheng, et al. Evidences for the regulation of GnRH and GTH expression by GnIH in the goldfish, Carassius auratus[J]. |
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