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1. Understanding the early ovarian developmental changes in the female chickens | |||
Ping Yu,Jian Li,Yuling Mi,Caiqiao Zhang | |||
Animal Husbandry, Veterinary Medicine 04 December 2015 | |||
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Abstract:The reproductive performance of female hen mainly depends on ovarian and follicular development. Therefore the complete insight of the mechanisms leading to the development of ovary is very important. In this respect we have studied early ovarian development until the formation of primordial follicles in the chicken. Our study was based on on the early ovarian development and then post hatching development of ovary. We have noticed asymmetric ovarian developmental phenomenon after E7.5 of embryonic chicken sex differentiation. The size of the right side ovary was not distinct and cortex disappeared. The right ovarian germ cell proliferation rate gradually decreased from E5.5 to E7.5 and some of them were crowded into the medullary lacunae by programmed cell death. In our another study we have noticed that the primordial follicle formation started to commence after the third day after hatching and it became rapid after first week of post hatching to two weeks of post hatching in the female chicken. In addition to this, we have observed an abnormal phenomenon during the formation of primordial follicle when a low number of the multiple oocyte follicles that were thought to be oocyte cysts and did not completely break apart during cyst breakdown due to the unknown reason. This underlying mechanisms needs further research. Therefore, our study of early ovarian development in the chicken will make a theoretical base for further understanding of the regulation of reproduction function and improving the laying performance in poultry. | |||
TO cite this article:Ping Yu,Jian Li,Yuling Mi, et al. Understanding the early ovarian developmental changes in the female chickens[OL].[ 4 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4669011 |
2. Distribution of the Multidrug Resistance Gene cfr in Staphylococcus Isolates from Pigs, Workers, and the Environment of a Hog market and a Slaughterhouse in Guangzhou, China | |||
LIN Dachuan,WANG Jing,WEI Hongkun,LIU Xiaoqin,LIU Jianhua,ZENG Zhenling | |||
Animal Husbandry, Veterinary Medicine 17 December 2014 | |||
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Abstract:The multi-resistance gene cfr has been widely disseminated among the livestock in China. A total of 444 Staphylococcus isolates recovered from pigs, workers, and the environment of a slaughterhouse and a hog market in Guangzhou, China, to investigate the prevalence and dissemination of cfr among staphylococci from different sources. 20 (4.5%) staphylococcal isolates, including one Staphylococcus cohnii, one Staphylococcus aureus, and 18 Staphylococcus simulans, harboured the cfr gene. The cfr-carrying S. aureus strain BKED isolated from the ground surface swab in hog market, exhibits spa type t889 and belongs to the clonal lineage ST9. SmaI pulsed-field gel electrophoresis (PFGE) analysis of 26 staphylococcal strains (22 S. simulans and 4 S. cohnii ), including previously reported cfr-carrying staphylococci of animal food origin, exhibited 19 major PFGE patterns A-S. Clonal spread of cfr between porcine strains and strains of human, the environment, food origins occurred. The genetic contexts of the cfr gene in plasmids pHNKF3, pHNZT2 and pHNCR35 from S. simulans of swine or human origin were very similar to that observed in other Staphylococcus species isolated from animal, human clinics, and animal food. In summary, clone spread and horizontal transmission via mobile elements were the two main ways leading to the dissemination of the multi-resistance gene cfr among staphylococcal isolates from different sources. Given the possibility that the cfr gene is transferred from animals to humans via the food chain, close contact and the environment, continued surveillance of this gene in pigs and workers in close contact with them is warranted. | |||
TO cite this article:LIN Dachuan,WANG Jing,WEI Hongkun, et al. Distribution of the Multidrug Resistance Gene cfr in Staphylococcus Isolates from Pigs, Workers, and the Environment of a Hog market and a Slaughterhouse in Guangzhou, China[OL].[17 December 2014] http://en.paper.edu.cn/en_releasepaper/content/4623852 |
3. The translocation of CIDEC in hepatocytes depends on nutritional status | |||
Zhou Lei,Li Hongqiang,Yang Zaiqing | |||
Animal Husbandry, Veterinary Medicine 15 January 2014 | |||
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Abstract:The CIDEC protein is located in lipid droplets (LDs) and the endoplasmic reticulum (ER), and is induced in fatty liver. However, the binding domain, the functional domain and the underlying mechanism of CIDEC in stimulating fat accumulation remain unclear. Here, we investigated the subcellular localization and function of pig CIDEC and confirmed CIDEC promotes unilocular development of LDs, reduces the specific surface area (SSA) of LDs and stimulates fat accumulation in HepG2 cells. By analyzing a series of CIDEC mutants, we revealed the N-domain (1-173 aa) is involved in LD localization and the C-domain (174-238 aa) is necessary for LD fusion. Further analysis indicated that the 106-173 aa region includes an ER-binding domain. Moreover, CIDEC stayed in the ER under lipid deficient conditions and translocated to LDs under fatty acid stimulation suggesting that localization of CIDEC in the ER is prior to the LD. Conclusions: Our data indicated excessive nutrition stimulated hepatic CIDEC expression and an increasing level of CIDEC induced hepatic LD fusion and fat accumulation. Our work suggests that CIDEC protects LDs by decreasing the SSA of LDs and is involved in the development of fatty liver. | |||
TO cite this article:Zhou Lei,Li Hongqiang,Yang Zaiqing. The translocation of CIDEC in hepatocytes depends on nutritional status[OL].[15 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4582429 |
4. miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing | |||
Zhou Jiawei,Liu Gang,Guan Kaifeng,Ma Changping,Lei Bin,Tong Keya,Li Jialian,Li Fenge | |||
Animal Husbandry, Veterinary Medicine 03 April 2013 | |||
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Abstract:miRNAs are a large family of small regulatory elements that direct messenger RNA degradation or disrupt mRNA translation. High-throughput Solexa sequencing approach was adopted to expand the repertoire of porcine miRNAs and to explore their roles on spermatogenesis. 237,590 total reads (792 unique reads) representing 65 known mature miRNAs in the miRBase release 14.0 were detected. 116 miRNAs including 12 known miRNAs in the miRBase release 15.0 and 104 novel miRNAs were identified from the un-annotated small RNAs. 466,757 targets for 104 novel miRNAs were predicted by MIREAP. The highly expressed miRNAs in porcine testes (let-7c, let-7f, miR-148a, miR-140* and miR-199b) had a very high rate of SNP mutation, and the most popular SNP was T to G mutation at position 6. Three known miRNAs including miR-514, miR-507, miR-508-5p were significantly differentially expressed between some groups (p<0.05), while two novel miRNAs- DB-m0102 and DB-m0071 were not significantly differentially expressed between all detected groups. | |||
TO cite this article:Zhou Jiawei,Liu Gang,Guan Kaifeng, et al. miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing[OL].[ 3 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4535593 |
5. Molecular cloning, characterization and inhibitory effect on B-cell survival of BAFF-R, a novel receptor of TNFR family in horse (Equus caballus) | |||
WU Haitao,KE Zhen,ZHANG Jianrong,XU Xinzhou,LIU Meng,ZHANG Shuangquan | |||
Animal Husbandry, Veterinary Medicine 07 February 2012 | |||
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Abstract:B-cell survival depends on signals induced by B-cell activating factor (BAFF) binding to its receptor (BAFF-R). In mice, mutations in BAFF or BAFF-R cause B-cell lymphopenia and antibody deficiency. However, the information about horse is poorly understood. Here, We report the identification and analysis of a novel horse TNF receptor related to B-cell suivival, BAFF-R. The horse BAFF-R gene spans 1444 bp, including three exons and located on chromosome 28. The horse BAFF-R protein is composed of 183 aa, with the calculated molecular weight 19 kDa and isoelectric point 6.12. As a type Ⅲ transmembrane protein, its extracellular domain has one cysteine-rich domain (CRD) containing four cysteine residues, and cytoplasmic domain exists the TRAF3 binding domain. The genetic relationship between horse and dog is the closest by the phylogenetic analysis of BAFF-R amino acids. Moreover, recombinant BAFF-R-Fc protein not only binds to B-cell, but also inhibits B-cell survival induced by BAFF in vitro. These studies provide the basis for investigation on its roles in regulating B lymphocyte survival and related immune diseases in horse. | |||
TO cite this article:WU Haitao,KE Zhen,ZHANG Jianrong, et al. Molecular cloning, characterization and inhibitory effect on B-cell survival of BAFF-R, a novel receptor of TNFR family in horse (Equus caballus)[OL].[ 7 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465365 |
6. Screening and Identification of Differentially Expressed Genes in Goose Hepatocytes Exposed to Free Fatty Acid | |||
Pan Zhixiong,Wang Jiwen | |||
Animal Husbandry, Veterinary Medicine 19 November 2010 | |||
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Abstract:The overaccumulation of triglycerides (TG) in hepatocytes induced hepatic steatosis, however, little is known about the mechanism of goose hepatic steatosis. The aim of this study was to define an experimental model of hepatocellular steatosis with TG overaccumulation and minimal cytotoxicity, using a mixture of various proportions of oleate and palmitate free fatty acids (FFAs) to induce fat-overloading, then using suppressive subtractive hybridization (SSH) and a quantitative PCR approach to identify genes with higher or lower expression levels after the treatment of cells with FFA mixtures as compared with untreated cells. Overall, 502 differentially expressed clones, representing 21 novel genes and 87 known genes, were detected by SSH. Based on functional clustering, up- and down-regulated genes were mostly related to carbohydrate and lipid metabolism, enzyme activity and signal transduction. The expression of 20 selected clones involved with carbohydrate and lipid metabolism pathways was further studied by quantitative PCR. The data indicated that six clones similar to the genes ChREBP, FoxO1, apoB, IHPK2, KIF1B and FSP27, which participate in de novo synthesis of fatty acid and secretion of very low density lipoproteins (VLDLs), had significantly lower expression levels in the hepatocytes of treated with FFA mixtures. Meanwhile, thirteen clones similar to the genes DGAT-1, ACSL1, DHRS7, PPARα, L-FABP, DGAT-2, PCK, ACSL3, CPT-1, A-FABP, PPARβ, MAT and ALDOB had significantly higher expression levels in the hepatocytes of treated with FFA mixtures. These results suggest that several metabolic pathways are altered in goose hepatocytes, which may be useful for further research into the molecular mechanism of goose hepatic steatosis. | |||
TO cite this article:Pan Zhixiong,Wang Jiwen. Screening and Identification of Differentially Expressed Genes in Goose Hepatocytes Exposed to Free Fatty Acid[OL].[19 November 2010] http://en.paper.edu.cn/en_releasepaper/content/4392163 |
7. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes | |||
Wu Xianshi,Wei Chengwu,Pan Cuiling,Duan Ying,Huang Kehe | |||
Animal Husbandry, Veterinary Medicine 09 November 2010 | |||
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Abstract:In this study, the effects of different forms and concentrations of Se on the regulation of glutathione peroxidase (GPx) activity and mRNA levels of phospholipid hydroperoxide GPx (GPx4) and type I deiodinase (D1) in chicken hepatocytes were evaluated. Primary cultured chicken hepatocyte monolayers derived from male White Leghorn chickens (aged 30-40 days) were incubated for 24 h with 0 (control), 0.5, 1, 1.5, 2, 3, 4 or 5 μmol/l of Se from DL-selenomethionine (Se-Met), Kappa-selenocarrageenan (Se-Car) or sodium selenite (Na2SeO3). Compared with controls, significant increases of GPx activity were observed in all hepatocytes treated with Se except for the hepatocytes treated with 5 μmol/l of Na2SeO3, with maximal effects at 2 μmol/l of Se-Met or Se-Car and 1.5 μmol/l of Na2SeO3, respectively. Significant decreases of GPx4 mRNA were observed in all hepatocytes treated with Se (vs. control). D1 mRNA was significantly increased in all groups treated with Se (vs. control), with maximal effects at 1.5 μmol/l of Se-Met and 0.5 μmol/l of Se-Car or Na2SeO3, respectively. Se-Met at doses of 1.5-5 μmol/l had greater effect on D1 mRNA than Se-Car and Na2SeO3 at equivalent doses. After resulting in the maximal effect, higher Se supplementation led to a dose-dependent reduction of GPx activity and D1 mRNA in all hepatocytes treated with Se. These results suggest that in chicken hepatocytes, the regulations of GPx and D1 of by different forms and concentrations of Se vary. | |||
TO cite this article:Wu Xianshi,Wei Chengwu,Pan Cuiling, et al. Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes[J].British Journal of Nutrition,2010,104(11):1605-1612. |
8. Effects of exogenous metallothionein on several endocrine hubs of dairy cattles | |||
Wu Lizhuan,Chen Haiyan,Zhang Bin,Li Lili,Xiao Bingnan,Chen Yuguang,Xiao Dingfu,Luo Jiajie,Wu Zongming | |||
Animal Husbandry, Veterinary Medicine 26 January 2010 | |||
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Abstract:To approach the effects of exogenous metallothionein (Zn-MT) on endocrine hubs of dairy cattles on the low-grade thermal stress, an experiment was conducted with 24 lactating cows, who were randomly allocated to groups A, B, C and D, and supplemented with 0, 6.0, 12.0 and 16.0mg Zn-MT•capita-1, respectively, by intravenous injection. The results showed that: The corrected milk yields in groups B, C and D were higher than those in groups A (P<0.05or P<0.01), the fat percentages of milk was improved significantly (P<0.05). The secretory capacity of INS in control group was lower than in experimental groups, especially in group B in 30d(P<0.01); the secretory capacity of IGF-Ⅰand T3 in groups B, C and D were higher than those in groups A (P<0.05or P<0.01), and in 45d, the same capacity in group C was the highest among all these numbers. The FSH in groups B and C constantly maintained low level, but the range of variation of FSH in group A was 4 times as same as in group C; The secretory capacity of Prog and E2 in experimental groups were higher than those of group A in anaphase (30~45d)(P<0.05); and the PRL in groups B, C and D were higher significantly than those in groups A (P<0.01), and the effect was faint in anaphase (P>0.05). Exogenous Zn-MT had the best effects on the thermoresistance of diary cattle 12.0mg•capita-1 by intravenous injection every 30~45 days. On one hand, the speed of catabolism and quantity of heat production in bodies were reduced by Zn-MT through adjusting secretory volume of endocrine hormone which is related to energy metabolism, on the other hand, to approach the healthful effects of thermoresistance, the gonadal hormones were controlled amplitude of variation, and on the same time, the secretory volume of PRL was improved by Zn-MT, which resulted in physiological status which was varied on balance for diary cattle. | |||
TO cite this article:Wu Lizhuan,Chen Haiyan,Zhang Bin, et al. Effects of exogenous metallothionein on several endocrine hubs of dairy cattles[OL].[26 January 2010] http://en.paper.edu.cn/en_releasepaper/content/39410 |
9. Numerical simulation of inspiratory airway in nasal cavity of Bactrian camels (Camelus bactrianus) | |||
Wang Hongju,Bai Zhongtian ,Wang Jianlin ,Gao Chengjuan | |||
Animal Husbandry, Veterinary Medicine 27 September 2008 | |||
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Abstract:To investigate the flow patterns in nasal cavity of Bactrian camel (Camelus bactrianus), we construct an anatomically accurate finite element model of its left nasal cavity from CT scans of a healthy adult nose. By numerically solving the steady-state Navier-Strokes and continuity equations, we study the flow distribution and the complete velocity field for inspiration throughout the nasal cavity under physiological flow rates of breathing. During inspiration, the highest velocity occurred along the nasal floor. | |||
TO cite this article:Wang Hongju,Bai Zhongtian ,Wang Jianlin , et al. Numerical simulation of inspiratory airway in nasal cavity of Bactrian camels (Camelus bactrianus)[OL].[27 September 2008] http://en.paper.edu.cn/en_releasepaper/content/24420 |
10. Effects of Crude Enzyme Preparation Supplemented | |||
Xiaojie Ai,Zhengkang Han | |||
Animal Husbandry, Veterinary Medicine 09 December 2005 | |||
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Abstract:Twenty four 7-day-old crossbred goslings (Chuan white×Tai lake) were divided randomly into control and treatment groups. Enzyme preparation was added in the diet of treatment group at dosage of 1 g/kg. The blood samples were collected from 60 day-old goslings via the wing vein. During the fifty-three days experiment, body weights were adopted measured each week, related hormone contents and biochemical parameters in plasma were determined. The results were as following:(1) Compared with control, body weight of treatment group were significantly higher (P<0.05) since 3-week-old goslings. The weight gains were significant higher (P<0.05) in 4~7 week-old goslings too, feed intake were increased and the “feed: body weight gain” was decreased; (2) Compared with control, growth hormone (GH) and insulin-like growth factor-I (IGF-I) levels were dramatically enhanced by 36.62 % (P<0.01) and 66.55 % (P<0.01) respectively, while the level of β-endorphin (β-END) decreased by 51.66 % (P<0.01); (3) Thyroid-stimulating hormone (TSH) and 3,3\ | |||
TO cite this article:Xiaojie Ai,Zhengkang Han. Effects of Crude Enzyme Preparation Supplemented [J].Journal of Shanghai Jiaotong University(Science) ,2005年,E-10卷,sup1期,115 ~ 119 |
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