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	1. Comparisons of extraction methods of microbial DNA from fermented grains
	ZHANG Yuhan,LI Guanhua,YUAN Lin
	Biology 19 May 2016
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	Abstract:Microbial populations in fermented grains during fermentation is the key to influence the flavor of Chinese liquor. In this paper, fermented grains samples from Hetao were used for DNA extraction, we studied the effects of six different extraction methods on total DNA extraction from fermented grains. The follow-up experiment showed that the obtained results in the use of the repeated freezing and thawing was the best for DNA extraction and polyacrylamide gel electrophoresis. Comparing with the other methods, the repeated freezing and thawing can reflect better the diversity of microorganism.
	TO cite this article:ZHANG Yuhan,LI Guanhua,YUAN Lin. Comparisons of extraction methods of microbial DNA from fermented grains[OL].[19 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4691595


	2. Characterization of Acetobacter pasteurianus CGMCC 3089 during evolutionary adaptation to ethanol
	Zheng Yu,Han Qi,Zhang Keping,Wang Min
	Biology 25 October 2012
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	Abstract:Acetobacter pasteurianus is usually used for vinegar fermentations, however, the growth of A. pasteurianus was commonly inhibited by high ethanol concentration. In this research, the ethanol resistance of A. pasteurianus CGMCC 3089 was improved by a continuous ethanol stress adaptation culture. The characterization of A. pasteurianus CGMCC 3089 during the evolutionary process of adaptation to ethanol was performed with respect to the improved resistance to ethanol. It was found that the ethanol resistance of A. pasteurianus was related to the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenases (ALDH). Furthermore, the improved resistance against ethanol was an inheritable phenotype, instead of a transient physiologic adaptation. The results of Random Amplified Polymorphic DNA (RAPD) suggested that there was some diversity between the genomes of the original strain and the evolutionary strain.
	TO cite this article:Zheng Yu,Han Qi,Zhang Keping, et al. Characterization of Acetobacter pasteurianus CGMCC 3089 during evolutionary adaptation to ethanol[OL].[25 October 2012] http://en.paper.edu.cn/en_releasepaper/content/4493041

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	3. High cell-density production of glucagon-like peptide-1 derivate (GP62) in recombinant E.coli by optimization of culture medium
	Jiang Deqi,Ran Yanhong,Chen Ying,Sang Yanxia,Zhou Tianhong,Li Hongjian
	Biology 11 February 2011
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	Abstract:This article aims to get high level expression of GLP-1 derivate (GP62) in high cell-density recombinant Escherichia coli BL21(DE3) by optimizing the fed-batch culture medium. The effects of different basal mediums, yeast extract and inorganic salts concentration on cell growth and expression of GLP-1 derivate (GP62) in recombinant E.coli BL21(DE3) were investigated by single factor test and orthogonal array. Based on the 2YT mediun, get the optimun 2YT-GP62 medium. The highest productivity of 30.0 mg l-1 GLP-1 derivate (GP62) and the ?nal cell density 21.3 (OD600) had been obtained in 5L fermentor induced with 0.6 mmol l-1 IPTG. The final cell density was 4.4 times higher than that in LB culture medium and the expression level of GP62 improved from 27.5% to 31.2%. The results showed that 2YT-GP62 medium can be used for recombinant GLP-1 derivate batch cultivation.
	TO cite this article:Jiang Deqi,Ran Yanhong,Chen Ying, et al. High cell-density production of glucagon-like peptide-1 derivate (GP62) in recombinant E.coli by optimization of culture medium[OL].[11 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4410546


	4. Optimal conditions for producing microalgal oil with high oleic acid content from Chlorella vulgaris LB 112
	Zhang Wei,Wu Hong,Zong Minhua
	Biology 07 July 2009
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	Abstract:Five different microalgae, including Chlorella vulgaris 24, Chlorella vulgaris LB112, Chlorella pyrenoidosa 27, Chlorella pyrenoidosa LB137, Chlorella pyrenoidosa LB308, were investigated for the purpose of screening out a strain producing lipid with high oleic acid content and Chlorella vulgaris LB112 was found to be able to accumulate lipid of high oleic acid content and unsaturated fatty acid content. Effects of culture conditions and culture medium components on the cell growth, lipid accumulation as well as lipid composition of Chlorella vulgaris LB112 were further investigated. The optimal carbon source, nitrogen source and C/N molar ratio were glucose, glycine, and 357, respectively. The supplement of small amount of divalent metal ions including Mg2+ and Fe2+ was beneficial to cell growth and lipid accumulation of Chlorella vulgaris LB112. The favorable temperature, initial pH of medium, light intensity and shaking rate were 25 °C, 6.0, 1000 lux and 130 r min-1, respectively. Under the optimum conditions, a biomass of 4.67 g l-1 and a lipid content of 47.58%, which are much higher than the original values (2.20 g l-1 and 34.55%) before optimization, could be obtained after culture for 7 days. The microalgal oil mainly contains 7.44% palmitic acid, 2.78% palmitoleic acid, 6.58% stearic acid, 68.10% oleic acid, 10.07% octadecadienoic acid, 4.02% octadecatrienoic acid and the total unsaturated fatty acids content reaches around 85%.
	TO cite this article:Zhang Wei,Wu Hong,Zong Minhua. Optimal conditions for producing microalgal oil with high oleic acid content from Chlorella vulgaris LB 112[OL].[ 7 July 2009] http://en.paper.edu.cn/en_releasepaper/content/33683


	5. Economical propionic acid production from cane molasses by Propionibacterium freudenreichii CCTCC M207015 in a plant fibrous-bed bioreactor
	Xiaohai Feng,Bo Wu,Hong Xu,Hanjie Ying,Pingkai Ouyang
	Biology 16 January 2009
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	Abstract:Propionic acid was produced in a plant fibrous-bed (PFB) bioreactor by Propionibacterium freudenreichii CCTCC M207015. Sugar cane bagasse and cane molasses, as the by-products of sugar-refinery, were used as cell immobilized material and carbon source respectively for propionic acid production. For the non-treated cane molasses, 62.08 g l–1 of propionic acid was produced after 254 h of fermentation with a productivity of 0.24 g h–1 l–1. Hydrolyzed cane molasses was also applied for the propionic acid production. The maxiumun propionic acid yield reached 91.87 g l–1 after 254 h fermentation with a productivity of 0.36 g h–1 l–1. The PFB bioreactor exhibited excellent production stability during batch fermentation and the propionic acid yield remained high after ten batch of propionic acid production.
	TO cite this article:Xiaohai Feng,Bo Wu,Hong Xu, et al. Economical propionic acid production from cane molasses by Propionibacterium freudenreichii CCTCC M207015 in a plant fibrous-bed bioreactor[OL].[16 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27931


	6. The positive influences of Mg2+, Mn2+and Zn2+ addition on the gamma-CGTase Production by Bucillus macorous
	Wang feng,Du guocheng,Cheng jian
	Biology 25 October 2007
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	Abstract:The effects of Mg2+, Mn2+ or Zn2+ addition on gamma-CGTase production by Bacillus macorous WSH02-06 were studied. The gamma-CGTase yield was increased maximally almost three-times by the addition of Mg2+, Mn2+ and Zn2+. The addition of these cations also stimulated cell growth. The consumption of Mg2+ was promoted in the cultures with the co-existance of Mg2+ with Mn2+ or/and Zn2+. The maximal gamma-CGTase yield and dry cell weight showed a linear increasing relation with the consumption of Mg2+. The increased intracellular concentration of ATP and ATP-to-ADP ratios by the addition of these cations can provide more energy for the better cell growth and higher gamma-CGTTase production.
	TO cite this article:Wang feng,Du guocheng,Cheng jian. The positive influences of Mg2+, Mn2+and Zn2+ addition on the gamma-CGTase Production by Bucillus macorous[OL].[25 October 2007] http://en.paper.edu.cn/en_releasepaper/content/15921


	7. Ethanol tolerance and the variation of plasma membrane composition of yeast floc populations with different size distribution
	Juanjuan Lei,Zhao Xinqing,Ge Xumeng,Bai Fengwu
	Biology 31 May 2007
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	Abstract:The ethanol tolerance of a self-flocculating yeast strain SPSC01 was investigated in an oxygen-limited fed-batch bioreactor. Employing Focused Beam Reflectance Measurement (FBRM) on-line monitoring system, four yeast floc populations with the average size ranging from 100 to 400μm were obtained. It was found that ethanol tolerance increased with the increasing floc size in the 100, 200, and 300μm floc populations, while increasing the average floc size further to 400μm resulted in lower ethanol tolerance. Examination of the membrane composition of different floc populations revealed that the plasma membrane composition of the floc populations was significantly different in the contents of ergosterol, phosphatidylinositol, as well as phospholipid palmitoleic acid. What’s more, the plasma membrane of more ethanol tolerant floc population was less permeable when subjected to 15% (V/V) ethanol shock treatment, and the plasma membrane ATPase activities were higher in the floc populations with higher ethanol tolerance. These results indicate that the average size distribution of the floc populations exerted great influence on the physiological status of yeast cells during the ethanol production process, leading to the changes in plasma membrane composition that contributed to improved ethanol tolerance in self-flocculating yeast SPSC01.
	TO cite this article:Juanjuan Lei,Zhao Xinqing,Ge Xumeng, et al. Ethanol tolerance and the variation of plasma membrane composition of yeast floc populations with different size distribution [OL].[31 May 2007] http://en.paper.edu.cn/en_releasepaper/content/13181


	8. Optimization of an enzyme production medium and bioconversion conditions for L (+)-tartaric acid production by Corynebaterium sp. in fed-batch culture
	Wenpeng Li,Jinlun Xie
	Biology 24 February 2007
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	Abstract:The medium for production of cis-epoxysuccinate hydrolase from Corynebaterium sp. YNUCC0211 and bioconversion conditions for L (+)-tartrate production were optimized by means of statistical methods respectively. Results revealed that the yields of L (+)-tartrate were improved by feeding fresh synthesized cis-expoxysuccinate solution with 12.0 of pH or with 3% of hydrogen peroxide to the optimum medium after being inoculated for 32-48 hours. The optimum medium contains (g/litre): glucose 15, cornsteep liquor 3.0, yeast extraction 0.3, K2HPO4.3H2O 3.5, dipotassium cis-epoxysuccinate 7.0, NH4NO3 4.0, (NH4)2 SO4 1.0, MgSO4.7H2O 1.0, FeSO4.7H2O 0.02, pH 7.0 - 7.2.
	TO cite this article:Wenpeng Li,Jinlun Xie. Optimization of an enzyme production medium and bioconversion conditions for L (+)-tartaric acid production by Corynebaterium sp. in fed-batch culture[OL].[24 February 2007] http://en.paper.edu.cn/en_releasepaper/content/11195

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