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	1. Effects of Bioefficacy of DL-2-Hydroxy-4-Methylthio-Butanoic Acid on Egg Production and Egg Quality in Laying Hens Fed on Medium or Low Nutrient Scheme
	Jianmei Wan,Jianping Wang,Xuemei Ding,Shiping Bai,Qiufeng Zeng,Huanwei Peng,Zhuowei Su,Yue Xuan,Keying Zhang
	Animal Husbandry, Veterinary Medicine 21 April 2017
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (4K B)

	Abstract:Two experiments were carried out to investigate the effects of bioefficacy of DL-2-Hydroxy-4-Methylthio-Butanoic Acid (HMTBA) for layers fed on medium or low nutrient level diets under restricted feeding (Exp.1) or ad libitum feeding (Exp.2) on egg production and quality. In Exp.1, a total of 2000 layers were assigned to 10 treatments in a completely randomized design, with 10 replicates per treatment, whilst in Exp.2 there were 10 replicates (15 hens per replicate) of 10 treatments. In all experiments, the bioefficacy of HMTBA was set at 80%, 84%, and 88% (on weight basis). In Exp.1, Methionine deficiency significantly (P < 0.05) reduced egg weight and eggshell weight regardless of the dietary nutrient level. Bioefficacy of HMTBA had no influence (P > 0.05) on any egg production or egg quality parameters of hens when compared with DL-methionine (DLM) treatments. In Exp.2, egg production, ADFI, egg weight, egg mass and feed conversion ratio significantly declined due to methionine deficiency (P < 0.01). There was no difference (P > 0.05) between HMTBA and DLM on egg production and egg quality parameters within each dietary nutrient level. In conclusion, bioefficacy of HMTBA can be set at 88% on weight basis regardless of dietary nutrient level.
	TO cite this article:Jianmei Wan,Jianping Wang,Xuemei Ding, et al. Effects of Bioefficacy of DL-2-Hydroxy-4-Methylthio-Butanoic Acid on Egg Production and Egg Quality in Laying Hens Fed on Medium or Low Nutrient Scheme[OL].[21 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4727788


	2. Effects of methionine source and level on hepatic methionine and lipids metabolism in laying hens fed with low or moderate nutrient level diet under restricted feeding
	Jianmei Wan,Jianping Wang,Xuemei Ding,Shiping Bai,Qiufeng Zeng,Huanwei Peng,Zhuowei Su,Yue Xuan,Keying Zhang
	Animal Husbandry, Veterinary Medicine 20 April 2017
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (4K B)

	Abstract:The study was carried out to investigate the effects of methionine source and level on hepatic methionine and lipid metabolism of commercial laying hens fed with low nutrient level (LN) or moderate nutrient level (MN) diets under restricted feeding. A completely randomized design was used. Within each nutrient level, there were 2 methionine sources (DL-methionine, DLM; DL-2-hydroxyl-4-methylthio-butiric acid, HMTBA) and 3 supplementation levels (LN: 0, 0.091 and 1%; MN: 0, 0.113 and 1%). A total of 2000 layers was allocated to 10 dietary treatments with 10 replicates for each. For hens fed LN diets: regardless of nutrient level, methionine source and level had no effects on concentration of Hcy in liver or plasma; hens treated with DLM had higher plasma Met and Tau concentration than those treated with HMTBA; when compared with the control group, adding methionine (HMTBA source) at 0.091% or 1% markedly increased the expression of GNMT; adding methionine (DLM source) at 1% significantly increased the expression of BHMT. Under the condition of feeding MN diets: plasma Met concentration significantly increased with methionine supplementation. Adding methionine (HMTBA source) at 0.113% or 1% significantly increased the expression of ADA and adding methionine (HMTBA source) at 0.113% significantly increased the expression of MS. When control diet was fed, hens fed LN diets had higher expression of BHMT and MS. When adding methionine (HMTBA source) at 1%, hens fed LN diets had higher expression of SAHH, MTHFR, CEBPβ, AdipoR2, INF-α and IL-1β. When adding methionine (DLM source) at 1%, feeding LN diet significantly increased the expression of TNF-α. In conclusion, methionine deficiency may increase the methionine regeneration by up-regulating the expression of genes involved in hepatic methionine metabolism. Under the condition of adding excess HMTBA, feeding low-nutrient diet may increase the folate-dependent pathway of methionine regeneration, SAMe-dependent transmethylation, fat synthesis and inflammatory cytokines in liver, and increase the potential risk of liver diseases.
	TO cite this article:Jianmei Wan,Jianping Wang,Xuemei Ding, et al. Effects of methionine source and level on hepatic methionine and lipids metabolism in laying hens fed with low or moderate nutrient level diet under restricted feeding[OL].[20 April 2017] http://en.paper.edu.cn/en_releasepaper/content/4727428


	3. The relative bio-efficacy of DL-methionine and DL-methionine hydroxyanalogue calcium saltcompared to L-methionine in broilers
	Peng Jialong,Gao Jun,Bai Shiping,Luo Yuheng,Ding Xuemei,Zeng Qiufeng,Wang Jianping,Zhang Keying
	Animal Husbandry, Veterinary Medicine 20 April 2017
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (4K B)

	Abstract:The relative bio-efficacy (RBE) of DL-methionine (DLM) and DL-methionine hydroxy analogue calcium salt (MHA-Ca) compared to L-Met was evaluated in this paper. A total of 2560 10-d-old Ross 308 male broilers were randomly allotted to 16 treatments with 8 replicates of 20 birds each.Birds were fed with either a corn-soybean meal-pea protein concentrate-based diet (Met：0.28,10-21d；0.26,22-42d with no methionine and cysteine supplementation), or methionine supplemented diets at0.03, 0.06, 0.10, 0.15, and 0.21% methionine from L-Met, or DLM, or MHA-Ca from 10 to 42 days of age based on the Met content (L-Met or DL-Met) or equal mass to MHA.The average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) in the period of 10 to 42 days were measured. Liver (at 42d) was collected to determine the redox state:H2O2, catalase (CAT), malondialdehyde (MDA), total glutathione (T-GSH), oxidized GSH (GSSG), reduced GSH (reGSH) and 8-hydroxyguanosine (8-OHDG). Supplement of Met elicited linear or quadratic improvements on ADG, ADFI and FCR with no different among the Met sources except FCR (P=0.0251, L-Met and DLM was better than MHA-Ca). The effect on carcass performance was similar to growth performance. Based on equal mass, the RBE of DLMand MHA-Ca to L-Met was 115.4and 87.6%,respectively based on ADG; 94.6 and 48.6%, respectively, based on FCR; 119.8% and 91.2%, respectively, based on breast weight; 107.4% and 79.5%, respectively, based on breast meat yield. The supplementation of methionine (regardless of source) increased the levelsof GSH (P<0.0001) and decreased the level of MDA(P<0.0001), CAT (P<0.0001) and 8-OHDG (P=0.0035) in broiler livers. Inconclusion, the RBE of DLM and MHA-Ca was 108% and 64% on a product base. MHA-Ca tended to have an advantage on anti-oxidative system which may partly compensate for the low efficiency on protein synthesis, while the relation between anti-oxidative ability and RBE needed further study.
	TO cite this article:Peng Jialong,Gao Jun,Bai Shiping, et al. The relative bio-efficacy of DL-methionine and DL-

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