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1. Effect of Neurokinin B on expression of GnRH and kisspeptin in the hypothalamus of rat | |||
Gao Xiaoxiao,Zhang Kaifa,Li Xiumei,Yang Chen,Luo Lei,Liu Ya,Fang Fugui,Zhang Xiaorong | |||
Animal Husbandry, Veterinary Medicine 06 May 2016 | |||
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Abstract:The aim of study is to investigate the effect of central administration of neurokinin B (NKB) on the timing of puberty onset and the expression levels of kisspeptin and gonadotrophin releasing hormone (GnRH) genes and proteins in the hypothalamus of rats. Prepubertal Sprague-Dawley rats were divided into three groups: NKB, vehicle, and control groups. The rats in the NKB group received intracerebroventricular injection of NKB solution, those in the vehicle group received injections of an equal volume of saline, and those in the control group did not receive any treatment. The rats were sacrificed, and the hypothalamus was obtained, sectioned, and used for fluorescence immunocolocalization of GnRH and kisspeptin immunoreactivity. Total RNA was extracted and quantified, and the DNA was analyzed using RT-PCR. The results of this study demonstrated that NKB treatment of prepubertal rats expedited the timing of vaginal opening (VO) and increased the ovary and uterus weights. Additionally, the hypothalamic expression of Kiss1 and GnRH mRNA and the number of GnRH-immunoreactive (ir) cells number significantly decreased (P < 0.05) in the arcuate nucleus (ARC) but increased in the paraventricularis nucleus (PVN) and median eminence (ME), while the number of kisspeptin-ir cells significantly increased (P < 0.05) in the ARC and periventricular nucleus (PeN) relative to the control. There were no differences (P > 0.05) in the number of kisspeptin-ir cells in the PVN. Overall, the results suggest that NKB plays a major role in modulating the activity of kisspeptin and GnRH during puberty. Nevertheless, both kisspeptin and GnRH serve different functions in the in the different nuclei of the hypothalamus during the onset of puberty. | |||
TO cite this article:Gao Xiaoxiao,Zhang Kaifa,Li Xiumei, et al. Effect of Neurokinin B on expression of GnRH and kisspeptin in the hypothalamus of rat[OL].[ 6 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4687301 |
2. Reconstruction of pig embryos with different lipids - a handmade model | |||
Wang Chengfei,Niu Yingjie,Chi Daming,Zeng Yaqiong,Li Juan | |||
Animal Husbandry, Veterinary Medicine 23 March 2016 | |||
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Abstract:Numerous neutral lipids were stored in cytoplasm of porcine oocytes in the form of lipid droplets (LDs). These LDs play an important role in vitro maturation and embryo development. Reconstructed embryos with different lipids were performed in present study to establish a model for study of LDs. Three groups of reconstructed embryos were obtained by hand-made cloning (HMC) and delipation: W-L: whole LDs in embryos; H-L: half LDs in embryos; R-L: removed LDs in embryos. After chemical activation (0h), reconstructed embryos were cultured in WOWs system for 6 days. The in vitro developmental competence of the three kinds of reconstructed was assessed. Results showed that: each kind of these three groups of embryos can develop to blastocyst stage. Therefore, the embryos with other content of LDs can be reconstructed in future studies. Then, the H-L group embryos, which as a model to study the roles of LDs and mitochondria in early development of embryos, were composed of three parts: a half normal oocyte (stained by MitoTracker Red CMXRos before bisecting) without nucleus, a half of delipated oocyte without nucleus and a donor cell. Following, 1-cell (12h), 2-cell (24h), 4-cell (36h) and blastocyst (144h) were collected to observe the distribution of LDs and mitochondria. The results showed that LDs and mitochondria were successfully tracked from 1-cell to blastocyst stage. Both LDs and mitochondria were only visible in normal cytoplasm at 1-cell stage. At 2-cell stage, mitochondria were uniformly distributed in the cytoplasm in 28% of the total number. But the LDs still not evenly distributed. However, both LDs and mitochondria were dispersed fully at 4-cell and blastocyst stage. In conclusion, reconstructed embryos with different lipids were appropriate models for lipids study, as well as investigate the interaction among lipids, mitochondria and other organelles. ????? | |||
TO cite this article:Wang Chengfei,Niu Yingjie,Chi Daming, et al. Reconstruction of pig embryos with different lipids - a handmade model[OL].[23 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4681821 |
3. Expression and methylation status of imprinted gene IGF2R in lung of deceased cloned transgenic dairy goat | |||
ZHANG Yanli,ZHANG Guomin,WAN Yongjie,Wang Feng | |||
Animal Husbandry, Veterinary Medicine 20 November 2014 | |||
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Abstract:Dairy goat is a good model for production of transgenic proteins in milk by somatic cell nuclear transfer (SCNT). However, animals produced from SCNT are often linked with lung deficiencies. Imprinted genes are important regulators of lung growth, and may be subjected to faulty reprogramming. In the present study, firstly, microsatellite analysis, PCR and DNA sequence identification were conducted to confirm that these three dead kids were genetically identical to the transgenic donor cells. Secondly, the CpG island methylation profiles of imprinted insulin-like growth factor receptor (IGF2R) gene was assessed by Bisulfite Sequencing PCR (BSP) in lungs of the three dead transgenic clones and normally produced lambs. In addition, the relative mRNA level of IGF2R was also determined by real-time PCR (RT-PCR). Results showed that IGF2R gene in the lung of dead cloned goat fetus showed abnormal hypermethylation and higher mRNA expression levels (P<0.05) comparing to the control, indicating that aberrant DNA methylation reprogramming may be one of the important factors for the death of transgenic cloned animals. | |||
TO cite this article:ZHANG Yanli,ZHANG Guomin,WAN Yongjie, et al. Expression and methylation status of imprinted gene IGF2R in lung of deceased cloned transgenic dairy goat[OL].[20 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4619349 |
4. Expression of Nerve Growth Factor (NGF), and Its Receptors TrkA and p75 in the Reproductive Organs of Laying Hens | |||
PU Shaoxia,QU Changwei,LI Chunmei | |||
Animal Husbandry, Veterinary Medicine 10 July 2014 | |||
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Abstract:In order to investigate the expression levels of nerve growth factor (NGF) and its receptors (TrkA and p75) in the prehierarchical follicle and oviducts of hens, five 130-day-old laying hens were examined by immunohistochemistry and RT-PCR analyses. NGF and its receptors were expressed in the theca cells and granulosa cells of prehierarchical follicles, and they also expressed in the epithelial cells of oviducts. The expressions of NGF mRNA were discrepant in different parts of the prehierarchical follicle and oviducts. The expression of NGF and p75 mRNA levels in large white follicle (LWF) were high, as well as TrkA in small yellow follicle (SYF). In oviduct, the expression of NGF is highest in infundibulum but lowest in magnum. These results suggest that NGF may play an important role in the regulation of reproduction in hens. | |||
TO cite this article:PU Shaoxia,QU Changwei,LI Chunmei. Expression of Nerve Growth Factor (NGF), and Its Receptors TrkA and p75 in the Reproductive Organs of Laying Hens[OL].[10 July 2014] http://en.paper.edu.cn/en_releasepaper/content/4603982 |
5. Expression profiles of key candidate genes involved in steroidogenesis during follicular atresia in the pig ovary | |||
Zengxiang Pan,Jinbi Zhang,Fei Lin,Xueshan Ma,Xuguang Wang,honglin Liu | |||
Animal Husbandry, Veterinary Medicine 20 April 2012 | |||
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Abstract:More than 99% of follicles in mammalian ovaries undergo a degenerative process known as atresia, and thus only a limited number of ovarian follicles actually ovulate after full growth and development. The endocrinological regulatory mechanisms involved in follicular development have been studied extensively, but the precise and systematic molecular mechanisms of steroidogenesis enzymes involved in atresia are unclear. In the present study, we examined whether and how the steroidogenesis enzymes are involved in porcine ovary follicular atresia. Expression of steroidogenic acute regulatory protein, CYP11, CYP17, 3β-hydroxysteroid dehydrogenase (3β-HSD), CYP19, as well as related pituitary and ovarian hormone receptors were quantified in ovaries. During porcine follicular atresia, expressions of P450 cholesterol side chain cleavage enzyme, progesterone and androgen receptors increased significantly during the late atretic stage, while the expression of aromatase and follicle-stimulating hormone receptors decreased significantly in the early stage. These data suggested that the regulation of aromatase by follicle-stimulating hormone might induce follicular atresia, and that progesterone and androgen production further promoted follicular atresia. Additionally, a correlation analysis indicated a large and complex interactive network among these genes and the endocrinological microenvironment of the follicles. Significant correlations were observed between expression of steroidogenic enzymes and their receptors, and also between progesterone and 17β-estradiol (E2) levels in follicular fluid. Taken together, these results suggest that CYP19 plays a role during early atresia by regulating the production of E2, whereas CYP11 and 3β-HSD increase atresia progression by increasing progesterone levels. | |||
TO cite this article:Zengxiang Pan,Jinbi Zhang,Fei Lin, et al. Expression profiles of key candidate genes involved in steroidogenesis during follicular atresia in the pig ovary[OL].[20 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4476052 |
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