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1. Characterization and isolation of estradiol degrading bacteria Acinetobacter sp. (7-1) Isolated from Changchun, China. | |||
YU Jian,ZHAO yanyang,HOU yue | |||
Biology 15 December 2020 | |||
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Abstract:17β- Estradiol, as a major environmental estrogen, is a serious threat to human health and ecological security. The MS medium containing 0.5 mM estradiol as the sole carbon source was used to isolate a new Gram-negative bacteria from the fishing pond. This screening strain can use as the sole carbon source and energy source such as estradiol and testosterone. After 16S rRNA analysis, the bacteria belong to Acinetobacter sp with 99.71% identity. The strain was Gram-negative bacilli, Spherical, Blunt round ends, Scattered or arranged in pairs, No bud, No flagella. Size 2.126μm×0.753μm; Conditions for optimum degradation of estradiol were 27℃, 150 rpm, and substrate concentration was 1 mM/L, pH7.5 and 1% inoculum. The highest degradation rate, the degradation rate can reach 85%. The strain could grow normally in 5 μg/mL auromycin, but not in 100 μg/mL ampicillin,50 μg/mL kanamycin,10 μg/mL streptomycin. The degradation rate of 17β- estradiol was 81.4%, that of testosterone was 64.6%, that of progesterone was 44.6%, and that of 17α- ethinyl estradiol was 62.3% by high performance liquid chromatography. | |||
TO cite this article:YU Jian,ZHAO yanyang,HOU yue. Characterization and isolation of estradiol degrading bacteria Acinetobacter sp. (7-1) Isolated from Changchun, China.[OL].[15 December 2020] http://en.paper.edu.cn/en_releasepaper/content/4753226 |
2. Pusillimonas oleiphila sp. nov., a new member of the genus Pusillimonas that degrades fuel oils at low temperature | |||
Ma Ting,Huang Lei,Li Guoqiang | |||
Biology 07 March 2011 | |||
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Abstract:The taxonomic position of a novel strain, designated T7-7T, was investigated. This strain was isolated at 10oC as a diesel-oil-degrading organism from seabed mud in the Bohai Sea. Previously, a polar lipid pattern with phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and unknown aminolipids AL1 as the primary components was found. Partial 16S rRNA gene sequence analysis indicated that this strain was related most closely to Pusillimonas ginsengisoli DCY25T (98.4% sequence similarity), Pusillimonas soli MJ07T(97.5%) and Pusillimonas noertemannii BN9T (96.7%). The levels of 16S rRNA gene sequence similarity between strain T7-7T and other recognized species of the family Alcaligenaceae were below 95.3%. This suggested that strain T7-7T represented a member of the genus Pusillimonas. Different methods for the construction of phylogenetic dendrograms separated this strain from other previously known strains of Pusillimonas. Fatty acid analysis demonstrated the presence of high concentrations of C16:0 and C17:0 cyclo. Strain T7-7T exhibited relatively low levels of DNA-DNA relatedness with P. ginsengisoli DCY25T(47.1%), P. soli MJ07T(44.5%) and P. noertemannii BN9T (38.6%). Based on the 16S rRNA gene sequence similarity to the strains of Pusillimonas, and other unique phenotypic properties such as fatty acid composition, polar lipid profiles, and DNA-DNA hybridization, T7-7T was classified as a new species with the name Pusillimonas oleiphila sp. nov. The strain type was designated as T7-7T (=CGMCC 1.6148 T =DSM 18250T). | |||
TO cite this article:Ma Ting,Huang Lei,Li Guoqiang. Pusillimonas oleiphila sp. nov., a new member of the genus Pusillimonas that degrades fuel oils at low temperature[OL].[ 7 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4414817 |
3. A new strain Lactobacillus silagius: identification and characteristics | |||
MA Ting,WANG Jun,FAN Yanhui,LI Guoqiang | |||
Biology 07 March 2011 | |||
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Abstract:A novel strain which could produce lactic acid under the thermophile temperature of 48 oC was isolated from the silage. The strain was designated as lactobacillus sp. MD-1. Cells of the strain were gram-positive, bar-shaped, non-mobile, facultatively anaerobic and catalase-negative. No spores were detected. The strain produced D- and L-lactic acid in a homofermentative mechanism. 16S rRNA analysis of the strain showed that it had the sequence similarity of 99.3% with strain L. farraginis, but L. farraginis was heterofermentative. Based on taxonomic and 16S rRNA data, strain MD-1 was named Lactobacillus silagius sp. nov. Non-denaturing PAGE of LDH stained for activity on D/L lactate showed the strain had D- and L-lactate dehydrogenase. The lactic acid yield of strain MD-1 was about 140g/L on condition of the optimal fermentation and could be applied in lactic acid production. | |||
TO cite this article:MA Ting,WANG Jun,FAN Yanhui, et al. A new strain Lactobacillus silagius: identification and characteristics[OL].[ 7 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4414811 |
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