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1. QCM biosensor for detection of small nucleic acid fragments: sandwich reaction and signal amplification by silver nanoclusters | |||
GUO Jia,JIANG Qifa,Rui-Qin Fang | |||
Chemistry 24 September 2021 | |||
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Abstract:This article presents a quartz crystal microbalance (QCM) biosensor for the determination of nucleic acids by modifying electrodes with cysteine (Cys), polyethylene glycol (PEG), and silver nanoclusters (AgNCs), which represents a new approach with increased specificity and sensitivity of detection. The probe deoxyribonucleic acid immobilized on the surface of the QCM gold electrode hybridizes with a cysteine conjugate to form a specific recognition layer. The unmodified sites are then non-specifically closed by polyethylene glycol. The target gene is subsequently captured by the immobilized probe, resulting in a change in frequency. The addition of silver ions with reducing agents in an ammonia environment to form silver nanoclusters of DNA-AgNCS was used to further extend the sensitivity of the QCM sensor. The results show that it is possible to expand the frequency variation of the QCM sensor by a factor of 63.6 using such a method. A linear relationship between frequency change and DNA concentration was observed in the range of 1 nM to 10 M of target gene concentration. Thus, this biosensor provides a low-cost and sensitive tool for detecting DNA. | |||
TO cite this article:GUO Jia,JIANG Qifa,Rui-Qin Fang. QCM biosensor for detection of small nucleic acid fragments: sandwich reaction and signal amplification by silver nanoclusters[OL].[24 September 2021] http://en.paper.edu.cn/en_releasepaper/content/4755569 |
2. Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect | |||
Litianyi Tang,Hong Liu | |||
Chemistry 19 March 2018 | |||
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Abstract:In this work, we report a method for the fabrication of nitrocellulose (NC) membrane with photonic crystal (PC) pattern for bio-detection. The membrane is prepared by imprinting the corresponding PC pattern into it through thermal Nano-imprint method. Aptamer beacon marked with fluorescein is used for detection of target molecules. Only one droplet (2-3μl) containing the aptamer-target complexes is dripped onto the nitrocellulose membrane. After evaporation (15-20min), the droplet is completely dried and the complexes form a coffee ring on the membrane. The ring keep growing with the increase of complexes concentration .As the aptamer beacon is marked with fluorescein and PC pattern has a fluorescence enhancement effect, the fluorescent signal on the coffee ring for detection is considerable improved. Fluorescent detection of adenosine triphosphate (ATP) is used for testing the application of this method. Owing to fluorescence enhancement effect of PC, the fluorescent signal for detection is improved and the limit of detection is 80.2μM for ATP. Therefore, we believe this method is promising for the fabrication of patterned photonic nitrocellulose membrane for bio-detection and point-of-care testing. | |||
TO cite this article:Litianyi Tang,Hong Liu. Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect[OL].[19 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4743982 |
3. A New Fluorescent Chemodosimeter Based on Phenylaniline for Fe3+/Cu2+ | |||
YUAN Ruili,ZHAO Xinyuan,Zhang Lifeng,Wei Liuhe,Yu Mingming,LI Zhanxian | |||
Chemistry 29 January 2013 | |||
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Abstract:In this paper, a fluorescent chemodosimeter p-(2,3,4,5-tetraephenyl)-phenylaniline (D) was synthesized, which shows excellent selectivity towards Fe3+/Cu2+. When FeCl3 or CuCl2 was added into the ethanol solution of D, it exhibits a phenomenon that a great decrease of emission intensity against environmental and biological samples. In addition, further experiments demonstrate that Fe(NO3)3 has the similar influence as FeCl3 while Fe2(SO4)3 has not on the emission spectra of D; as for copper salts, copper acetate has the similar influence as CuCl2 while Cu(NO3)2 and CuSO4 cannot make the emission intensity of D quench completely. | |||
TO cite this article:YUAN Ruili,ZHAO Xinyuan,Zhang Lifeng, et al. A New Fluorescent Chemodosimeter Based on Phenylaniline for Fe3+/Cu2+[OL].[29 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4516424 |
4. Electrochemical Detection of Nitric Oxide on a SWCNT/RTIL Composite Gel Microelectrode | |||
Chang Ming Li,Jianfeng Zang,Dong Ping Zhan,Wei Chen,Sun Changqing,Ai. L. Teo,Yek. T. Chua, Vee. S. Lee, Shabbir. M. Moochhala | |||
Chemistry 27 April 2006 | |||
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Abstract:Single walled carbon nanotubes (SWCNT) and room temperature ionic liquid (RTIL) were used to make a gel microelectrode for studies of the oxidation of nitric oxide (NO). The Faraday response of the gel microelectrode was contributed from two components: an outside-surface microdisk and a thin-layer cell formed by inner porous electrode materials, and enhanced by the thin-layer effect. An EC mechanism, electrochemical NO oxidation followed by a chemical oxidation, was proposed. The gel microelectrode with a Nafion® coating eliminated interferences from nitrite and some biomolecules, improved stability, and had a linear response range from 100 nM to 100 µM. | |||
TO cite this article:Chang Ming Li,Jianfeng Zang,Dong Ping Zhan, et al. Electrochemical Detection of Nitric Oxide on a SWCNT/RTIL Composite Gel Microelectrode[OL].[27 April 2006] http://en.paper.edu.cn/en_releasepaper/content/6429 |
5. O-floating adn O-diffusing at metal surfaces | |||
SUn Changqing | |||
Chemistry 08 September 2005 | |||
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Abstract:The obsercved O-floating or diffusing at metal surfaces are attributed to the processed of tetrahedron bond switching under a certain conditions. | |||
TO cite this article:SUn Changqing. O-floating adn O-diffusing at metal surfaces[OL].[ 8 September 2005] http://en.paper.edu.cn/en_releasepaper/content/2824 |
6. Cu3O2 pairing tetrahedron at Cu(001) surface: aging and relaxation | |||
Sun Changqing | |||
Chemistry 06 September 2005 | |||
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Abstract:It is shown that the Cu3O2 paring tetrahedron formed at Cu(001) surface will suffer dehybridization under annealing. | |||
TO cite this article:Sun Changqing. Cu3O2 pairing tetrahedron at Cu(001) surface: aging and relaxation[OL].[ 6 September 2005] http://en.paper.edu.cn/en_releasepaper/content/2802 |
7. Electronic process of Cu(Ag, V, Rh)-(001) surface oxidation: atomic valences and bonding kinetics | |||
Sun Changqing | |||
Chemistry 09 August 2005 | |||
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Abstract:The electronic processes of Cu(Ag, V, Rh)(001) surface oxidation are comparatively analyzed based on the recent ‘chemical bond – valence band – potential barrier’ (BBB) correlation mechanism [C. Q. Sun, Prog. Mater. Sci. 48, 521-685 (2003)], which allows reaction formulae for all the observed phases with identification of individual atomic valence and the binding kinetics at the surfaces with the same geometry. It is consistently understood that the forming kinetics of the primary oxide tetrahedron and its derivative on the valence density-of-states are intrinsically common for all these analyzed systems, though the patterns of observation in terms of morphology and crystallography vary from situation to situation. However, the lattice size and electronegativity of the host surfaces determine extrinsically the site selectivity of the oxygen, the order of bond formation and the orientation of the oxide tetrahedron. | |||
TO cite this article:Sun Changqing. Electronic process of Cu(Ag, V, Rh)-(001) surface oxidation: atomic valences and bonding kinetics[OL].[ 9 August 2005] http://en.paper.edu.cn/en_releasepaper/content/2631 |
8. Estimating the extent of surface oxidation by measuring the porosity dependent dielectrics of oxygenated porous silicon | |||
L. K. Pan,Sun Changqing,C. M. Li | |||
Chemistry 02 August 2005 | |||
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Abstract:Surface oxidation and porosity variation play significant roles in the dielectric performance of porous silicon (PS) yet discriminating the contribution of these events is a challenge. Here we present an analytical solution that covers contributions from the components of silicon oxide surface, silicon backbone and voids using a serial-parallel capacitance structure. Agreement between modeling predictions and measurement has been realized, which turns out an effective method that enables us to estimate the extent of surface oxidation of a specimen by measuring the porosity dependent dielectric response of the chemically passivated PS, and provides guidelines that could be useful for designing dielectric porous structures with surface oxidation. | |||
TO cite this article:L. K. Pan,Sun Changqing,C. M. Li. Estimating the extent of surface oxidation by measuring the porosity dependent dielectrics of oxygenated porous silicon [OL].[ 2 August 2005] http://en.paper.edu.cn/en_releasepaper/content/2587 |
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