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1. Altered expression of plant miRNAs and their target mRNAs in tomato flower and fruit upon virus infections | |||
Lin Ruohong ,Junli Feng,Yuwei Wang,Jianning Liu,Jishuang Chen | |||
Biology 28 December 2011 | |||
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Abstract:The economic importance of tomato is well documented, and tomato has become a model for fleshy fruit development and ripening studies. Plant microRNAs (miRNAs) are small endogenous RNAs that are involved in a variety of activities, including plant development, signal transduction and protein degradation, as well as response to environment stress, and pathogen invasion. Here in this study, we aimed at quantifying the expression alterations of nine miRNAs and target mRNAs in tomato flower and fruit development upon CMV and TAV infections. Three different Cucumber mosaic virus (CMV) strains CMV-Fny, CMV-Fny2b, CMV-Fny-satT1 and Tomato aspermy virus (TAV)-Bj were used in our investigation, and the miRNA/mRNA expression alterations were analyzed by real-time quantitative RT-PCR. The results shown the levels of several miRNA/mRNA pairs were increased upon virus infection. However, the increased levels of individual miRNAs differed for different virus strains, reflecting differences in severity of symptom phenotypes. The altered expression pattern of these miRNA/mRNA pairs and their predicted functions indicate the possible roles in flower and fruit development, and provide experimental data for understanding the miRNA-mediated phenotype alterations in tomato fruit. | |||
TO cite this article:Lin Ruohong ,Junli Feng,Yuwei Wang, et al. Altered expression of plant miRNAs and their target mRNAs in tomato flower and fruit upon virus infections[OL].[28 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4457683 |
2. Different interactions between Cucumber mosaic virus and Tomato mosaic virus in tomato varieties | |||
Yixing Li,Guo Hong,Chen Shaoning,Chen Jishuang | |||
Biology 15 January 2010 | |||
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Abstract:Different interactions between CMV (Cucumber mosaic virus) and ToMV (Tomato mosaic virus) had been obtained in several tomato varieties. When tomato cv. Hezuo 903 plants were first inoculated with CMV-Fny, and then followed by a second inoculation with ToMV-N5 after 6-8 days, plants with co-infection expressed CMV-Fny-induced symptoms. The ToMV-N5-induced necrosis just localized on the inoculated leaves. Typical interference could be summarized for this phenomenon. Systemic necrosis induced by ToMV-N5 and interference between CMV-Fny and ToMV-N5 could occur in most tomato varieties (Hangza 1 and Zheza 809) except Zhongshu 4. They performed mild mosaic and synergism instead. The transcription of defense-related genes (PR-1a1 and PR-2) had been detected in tomato cv. Hezuo 903 plants among interference reaction. Results showed that two PR genes did not express in inoculated plants with mosaic and leaf deformation symptoms but was activated in ToMV-N5 infected plants with necrosis symptoms. It indicated that these two genes were effective with ToMV-N5 infection and could be interfered by pre-inoculation with CMV-Fny. Genotypes of resistance gene Tm2 in tomato cv. Zhongshu 4 and Hezuo 903 had been identified. Results revealed that two tomato varieties genotypes were both consistent with symptoms induced by ToMV-N5. It demonstrated that there may be a certain relationship between infection symptoms caused by ToMV-N5 on these two tomato varieties and genotype of Tm2. | |||
TO cite this article:Yixing Li,Guo Hong,Chen Shaoning, et al. Different interactions between Cucumber mosaic virus and Tomato mosaic virus in tomato varieties[OL].[15 January 2010] http://en.paper.edu.cn/en_releasepaper/content/39033 |
3. The fragment containing CP gene of Cucumber mosaic virus determines the severe leaf distortion phenotype expression on N. tabacum | |||
Xu Gang,Wu Peng,Liao Qian-sheng,Chen Ji-shuang | |||
Biology 04 March 2009 | |||
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Abstract:The Cb7 isolate of Cucumber mosaic virus (CMV) induces severe leaf distortion on Nicotiana tabacum in contrast to CMV-Fny that causes systemic mosaic in this host. It was found that N. tabacum plants infected with the reassortant, a combination of transcripts derived from cDNAs of CMV-Fny RNA1 and 2 (F1 and F2) and of CMV-Cb7 RNA3 (F12C3), displayed severe leaf distortion symptoms characteristic of the wild-type Cb7 infection, indicating that genomic RNA3 had a distinctive role in the determination of the symptom. An exchange of the fragment containing CP gene between Cb7 and Fny constructed two chimeric RNA3s: CFB, including CP gene of Fny, and FCB, including CP gene of Cb7. Each chimera mixed with F1 and F2 was inoculated onto N. tabacum. The F12CFB-infected plants expressed systemic mosaic symptoms, while the F12FCB-infected ones showed severe leaf distortion symptoms. The results suggested that the genetic determinant of the severe leaf distortion symptom showing on N. tabacum was exclusively associated with the fragment containing CP gene in the Cb7 genome. Accumulation of CMV RNAs in the systemic leaves of N. tabacum plants infected with the reassortant was determined using Northern blotting. The results showed that the accumulation of subgenomic RNA4 of F12FCB was significantly higher than that of F12CFB and Fny, as a control, and the amounts of RNA4 of F12CFB were equivalent to those of Fny. Therefore, it had been inferred that the high amounts of viral subgenomic RNA4, presenting high accumulation of CP, might be responsible for the severe leaf distortion symptom expression on N. tabacum. | |||
TO cite this article:Xu Gang,Wu Peng,Liao Qian-sheng, et al. The fragment containing CP gene of Cucumber mosaic virus determines the severe leaf distortion phenotype expression on N. tabacum[OL].[ 4 March 2009] http://en.paper.edu.cn/en_releasepaper/content/29878 |
4. Cucumber mosaic virus-mediated Regulation of Disease Development Caused by Tomato mosaic virus in Tomato Hosts | |||
Hong Guo,Shaoning Chen,Rong Zeng,Zhiyou Du,Zhenxiao Zhang,Weimin Zhu,Jishuang Chen | |||
Biology 02 March 2009 | |||
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Abstract:Cucumber mosaic virus (CMV) and Tomato mosaic virus (ToMV) are two principal viruses infecting tomato (Lycopersicon esculentum Mill). N5 is a necrosis strain of ToMV. In this work, interference between these two virues was analyzed in tomato cv. Hezuo 903. Plants were first inoculated with CMV-Fny or CMV-Fny∆2b, respectively. 7 days postinoculation (dpi), a second challenge inoculation was performed with ToMV-N5. The results showed that plants can be protected against challenge with ToMV-N5 by initial CMV-Fny infection, but not by CMV-Fny2b. The accumulation of these three viruses in single and double infections was detected by real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) assays. The real-time RT-PCR values indicated that ToMV-N5 accumulation was significantly suppressed by double infection with CMV-Fny in the second true leaves. The interaction between PVX and ToMV was further studied. The host reaction of PVX-BJ and ToMV-N5 double infections was almost the same as in ToMV-N5 singly infections. Based on above results, we speculate that the 2b protein may be an important determinant involved in CMV-mediated regulation of disease development caused by ToMV-N5 in tomato cv. Hezuo 903. | |||
TO cite this article:Hong Guo,Shaoning Chen,Rong Zeng, et al. Cucumber mosaic virus-mediated Regulation of Disease Development Caused by Tomato mosaic virus in Tomato Hosts[OL].[ 2 March 2009] http://en.paper.edu.cn/en_releasepaper/content/29796 |
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