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1. Fate and Removal of Various Antibiotic Resistance Genes in Typical Pharmaceutical Wastewater Treatment Systems | |||
Zhai Wenchao,Mao Daqing,Luo Yi | |||
Environmental Science and Techology 25 December 2015 | |||
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Abstract:The high levels of antibiotic residues in pharmaceutical wastewater treatment plants (PWWTPs) make these plants the hotspots for the proliferation of antibiotic resistance genes (ARGs). This study investigated the fate and removal of 11 subtypes ARGs for sulfonamide, tetracycline, β-lactam and macrolide resistance in each stage of two full-scale PWWTPs in northern China. The levels of typical ARG subtypes in the final effluents ranged from (2.56±0.13)×10^1 to (2.36±0.11)×10^7copies/ml. The absolute abundance of ARGs in effluents accounted for only 0.03%-78.1% of influents of the two PWWTPs, while the majority of the ARGs were transported to the dewatered sludge with concentrations from (2.65±0.43)×10^5 to (4.27±0.03)×10^10copies/g dry weight (dw). The total loads of ARGs discharged through dewatered sludge plus effluent was 1.01-14.09 folds higher than that in the raw influents, suggesting the proliferation of ARGs occurred in the wastewater treatment. The proliferation of ARGs mainly occurs in biological treatment process, such as aeration tank, anoxic tank, sequencing batch reactor (SBR) and bio-contact oxidation, facilitates the proliferation of various ARGs, implying significant replication of certain subtypes of ARGs may be attributable to microbial growth. Chemical oxidation seems promising to remove ARGs, with removal efficiency ranged from 29.3% to 85.7%, while the partial correlation analysis showed significant correlations between antibiotic concentration and ARG removal. Thus the high antibiotic residues within the PWWTPs may have an influence on the proliferation, fate and removal of the associated ARG subtypes. | |||
TO cite this article:Zhai Wenchao,Mao Daqing,Luo Yi. Fate and Removal of Various Antibiotic Resistance Genes in Typical Pharmaceutical Wastewater Treatment Systems[OL].[25 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4673578 |
2. Microbial enzym and biomass responses:deciphering vermifiltration treating excess sludge fed continuosly using Eisenia fetida despite seasonal variations | |||
XING Meiyan,Sun Wen,XU Zhe | |||
Environmental Science and Techology 26 August 2014 | |||
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Abstract:This paper reports on a seasonal pattern comparison of microbial enzymatic activities and biomass responses based on a conventional biofilter (BF, without earthworm) and a vermi?lter (VF, with earthworm, Eisenia fetida) for sewage sludge treatment fed continuously. The VSS reduction, viable cell number and enzyme activities were assayed to probe what made the VF operated stably. The results indicated that the earthworms' activities can further the volatile suspended solids (VSS) reduction with a 27.17% more than BF. Though the VF has a lower level in the viable cell number, the earthworms strongly improved the enzyme activities, such as INT- dehydrogenase, protease, β-glucosidase and amylase, which can explain the excellent performance of VSS reduction, compared with the BF. The correlation analysis documented that the VSS reduction was positively correlated with microbial enzyme activities. More importantly, the earthworm enabled the VF to avoid the detrimental influence of temperature, which guaranteed a stable performance during seasonal variations. | |||
TO cite this article:XING Meiyan,Sun Wen,XU Zhe. Microbial enzym and biomass responses:deciphering vermifiltration treating excess sludge fed continuosly using Eisenia fetida despite seasonal variations[OL].[26 August 2014] http://en.paper.edu.cn/en_releasepaper/content/4607574 |
3. Community Analysis of Halophilic Bacterial Consortium Capable of Degrading Phenol over a Wide Range of Salinity and the Degradation Pathway | |||
HUANG Zhongzi,WANG Ping,LI Hui,LIN Kuangfei,LU Zhiyan,GUO Xiaojue,LIU Yongdi | |||
Environmental Science and Techology 15 June 2014 | |||
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Abstract:A moderately halophilic bacterial enrichment was able to degrade 120 mg/L of phenol in the presence of 1-2 M of NaCl within 3 d or 2.5-3 M of NaCl within 6 d. The optimal degradation was achieved at 1.5 M of NaCl and 350 mg/L of phenol. PCR-DGGE profile of the enrichment showed that the Acidobacterium sp. and Chloroflexus sp. dominated the community. The phenol-biodegradation pathways consisted of an initial oxidative attack by phenol hydroxylase, and subsequent ring fission by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that ectoine and hydroxyectoine were the main compatible solutes to adjust the bacterial osmotic pressure. This study provides further information on the understanding of phenol-degradation over a wide range of salinity and remediation of phenol as a pollutant in the environment. | |||
TO cite this article:HUANG Zhongzi,WANG Ping,LI Hui, et al. Community Analysis of Halophilic Bacterial Consortium Capable of Degrading Phenol over a Wide Range of Salinity and the Degradation Pathway[OL].[15 June 2014] http://en.paper.edu.cn/en_releasepaper/content/4600166 |
4. Biodegradation of sulfamethoxazole by Phanerochaete chrysosporium | |||
GUO Xiali,ZHU Zhengwei,LI Hongli | |||
Environmental Science and Techology 10 February 2014 | |||
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Abstract:In this paper, degradation of sulfamethoxazole(SMX) by the white-rot fungus Phanerochaete chrysosporium was assessed. P. chrysosporium had a stronger tolerance for SMX in the concentration range of 10-30mg/L. The degradation percentage of SMX in liquid medium was 53% after 24h and reached 74% after 10 days when SMX was added at 10mg/L. Crude laccase produced by P. chrysosporium was used to degrade SMX in vitro. The results showed that the removal of SMX obviously increased with laccase activity and reaction time increasing. | |||
TO cite this article:GUO Xiali,ZHU Zhengwei,LI Hongli. Biodegradation of sulfamethoxazole by Phanerochaete chrysosporium[OL].[10 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4584992 |
5. Eco-physiological characteristics of three emergent macrophyte species to concentration changes of COD, nitrogen and phosphorus | |||
Sun Ruilian,Liu Jian | |||
Environmental Science and Techology 10 September 2013 | |||
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Abstract:Three macrophyte species (Zizania latifolia Turcz., Iris pseudacorus L. and Typha latifolia L.) were studied in constructed microcosm wetlands in order to compare the ability of hydrophytes in purifying simulated polluted river water and to analyze plant responses to oxidative stress induced by chemical oxygen demand (COD), nitrogen and phosphorus. The results showed that planted microcosms were more effective in removing COD, total nitrogen (TN) and total phosphorus (TP) than unplanted microcosms. Among the three hydrophytes, Z. latifolia was superior for TN removal, while I. pseudacorus units showed the highest TP removal efficiency. However, there was no significant difference between planted units in COD removal efficiencies. The plasticity of I. pseudacorus was the best exposed to sewage, which was associated with significantly higher SOD and CAT activities with treatment T1. However, treated with low COD/N/P (T1), the MDA levels of T. latifolia and Z. latifolia were basically unaffected. In addition, treatment of polluted river water induced enhancement in proline level of three species. It can be concluded that the antioxidative defence system and free proline accumulation were activated but could not resist the oxidative stress in plants exposed to high concentrations of COD/N/P (≥160/30/4 mg L-1). | |||
TO cite this article:Sun Ruilian,Liu Jian. Eco-physiological characteristics of three emergent macrophyte species to concentration changes of COD, nitrogen and phosphorus[OL].[10 September 2013] http://en.paper.edu.cn/en_releasepaper/content/4559293 |
6. Expression of anti-hyperglycaemic peptide MC6 from Momordica Chanrantia in Escherichia coli | |||
LIU Sixiu,SUI Guodong,FU Zhongping,MU Ruimin | |||
Environmental Science and Techology 12 January 2013 | |||
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Abstract:An anti-hyperglycaemic peptide MC6 [accession:AAX06814, B.nage et al, patent US 6852695] from Momordica Chanrantia with 18 amino acids was converted into nucleic acid sequence MC and synthesized according to E. coli prefer codons. Subsequently, MC was ligated to pET32a and expressed in E.coli. Recombinant protein pET-MC (about 21KD) was purified and analyzed by 12% SDS-PAGE. It showed that expressed recombinant pET-MC had good solubility in aqueous media. This result may provide such possibility that the bio-engineering bacteria methods can be used in the preparation of anti-diabetes medicines. | |||
TO cite this article:LIU Sixiu,SUI Guodong,FU Zhongping, et al. Expression of anti-hyperglycaemic peptide MC6 from Momordica Chanrantia in Escherichia coli[OL].[12 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4514714 |
7. Degradation of biphenyl and PCBs by isolated microorganism | |||
Qi Yun,Zhao Lin,Bao Shilong,Liang Wenjuan,Liu Shejiang | |||
Environmental Science and Techology 22 October 2012 | |||
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Abstract:Biphenyl is natural and synthetic aromatic compounds. This pollution has caused pubic concern due to its wide-spread use, improper handling, as well as its low degradability, strong bioaccumulation and high toxicity in the environment. Two bacterium strains, G-1 and G-2, were isolated from an activated sludge of a chemical factory wastewater treatment plant in China. Both the strains can use biphenyl as sole carbon and energy sources. The G-1 almost complete degraded it within 7 days, but can not utilized 3,3',4,4'-tetrachlorobiphenyl (PCB77) as sole carbon source. Although, train G-2 degrade biphenyl slower than G-1, it can utilize PCB 77. Strain G-2 was identified as Paracoccus according to phylogenetic similarity of 16S rRNA gene sequence. | |||
TO cite this article:Qi Yun,Zhao Lin,Bao Shilong, et al. Degradation of biphenyl and PCBs by isolated microorganism[OL].[22 October 2012] http://en.paper.edu.cn/en_releasepaper/content/4492400 |
8. Identification and characterization of cell wall proteins of a toxic dinoflagellate Alexandrium catenella using 2-D DIGE and MALDI TOF-TOF mass spectrometry | |||
WANG Dazhi,DONG Hongpo,LI Cheng,XIE Zhangxian,LIN Lin,HONG Huasheng | |||
Environmental Science and Techology 30 March 2011 | |||
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Abstract:Cell wall is an important subcellular component of dinoflgaellate cells in regard to various aspects of cell surface associated ecophysiology but the full range of cell wall proteins (CWPs) and their functions remain to be elucidated. This study identified and characterized CWPs of a toxic dinoflagellate, Alexandrium catenella using a combination of 2-D fluorescence difference gel electrophoresis (DIGE) and MALDI TOF-TOF mass spectrometry approaches. Using sequential extraction and temperature shock methods, sequentially extracted CWPs and protoplast proteins were separated from A. catenella, respectively. Comparison between sequentially extracted CWPs labeled with Cy3 and protoplast proteins labeled with Cy5, 120 CWPs were confidentially identified in the 2-D DIGE gel. These proteins gave positive identification of protein orthologues in the protein database by de novo sequence analysis and homology-based search. Majority of the prominent CWPs identified were hypothetical or putative proteins with unknown function or no annotation, while cell wall modification enzymes, cell wall structural proteins, transporter/binding proteins, signaling and defense proteins were tentatively identified in agreement with the expected role of the extracellular matrix in cell physiology. This work represents the first attempt to investigate CWPs of dinoflagellates which provides a potential tool for future comprehensive characterization of CWPs and elucidation of physiological functions of CWPs in dinoflagellates. | |||
TO cite this article:WANG Dazhi,DONG Hongpo,LI Cheng, et al. Identification and characterization of cell wall proteins of a toxic dinoflagellate Alexandrium catenella using 2-D DIGE and MALDI TOF-TOF mass spectrometry[OL].[30 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4419286 |
9. Proteomics of a toxic dinoflagellate Alexandrium catenella DH01: Detection and identification of cell surface proteins by fluorescent labeling | |||
LI Cheng,WANG Dazhi,XIE Zhangxian,DONG Hongpo,HONG Huasheng | |||
Environmental Science and Techology 27 March 2011 | |||
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Abstract:Alexandrium catenella DH01 is a toxic dinoflagellate species which is not only able to produce paralytic shellfish toxins(PSTs) but also cause harmful algal blooms (HABs) in the coast of China. In this study, we present a new protocol for specific labeling and detection of cell surface proteins (CSPs) of A. catenella DH01 cells using CyDye DIGE Fluor minimal dyes, and have identified CSPs using a combining approach of two dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF mass spectrometry. The results showed that the fluorescent cyanine dye Cy3 could specific combine with CSPs of A. catenella DH01 cells with minimal labeling of intracellular proteins. Trizol method was the best to extract CSPs with respect to protein spot number and resolution among the three protein extraction methods. A total of 41 CSPs were separated and identified from A. catenella DH01 in 2-D gel, among of them, 14 proteins gave the positive identification of protein orthologues in the protein database by MALDI TOF-TOF MS analysis. This work represents the first attempt to investigate CSPs of A. catenella using CyDye DIGE fluors dyeing method, which provides a potential important tool for future comprehensive characterization of CSPs and elucidation of physiological functions of CSPs in dinoflagellatess. | |||
TO cite this article:LI Cheng,WANG Dazhi,XIE Zhangxian, et al. Proteomics of a toxic dinoflagellate Alexandrium catenella DH01: Detection and identification of cell surface proteins by fluorescent labeling[OL].[27 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4418677 |
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