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1. Characterization of Akirin in the innate immune response of the kuruma shrimp Marsupenseus japonicus | |||
LIU Ning,SUN Jiejie,WANG Xianwei,WANG Lei,ZHANG Hongwei,ZHAO Xiaofan,WANG Jinxing | |||
Aquatic Science 27 November 2015 | |||
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Abstract:Akirin as a conserved nuclear protein functions in parallel with the NF-κB of the immune deficiency (IMD) pathway and the Toll-like receptor (TLR) pathway. In this study, the full-length cDNAs of Akirin and 14-3-3 in Marsupenaeus japonicus, named as MjAki and Mj14-3-3 were identified separately. MjAki was mainly expressed in hemocytes, heart and intestine and Mj14-3-3 was detected in all six tested tissues including hemocytes, heart, hepatopancreas, gills, stomach, and intestine. The expression of MjAki was upregulated in the hemocytes of shrimp challenged with Vibrio anguillarum and white spot syndrome virus (WSSV), Mj14-3-3 was first decreased and then increased in the shrimp challenged with V. anguillarum and no obviously change in the shrimp challenged with WSSV. The expression of some AMPs was obviously suppressed by knockdown of MjAki and Mj14-3-3 with RNA interference. The interaction of MjAki with Mj14-3-3, dorsal, relish was confirmed in vitro. We proposed that MjAki combined with Mj14-3-3, dorsal and relish, acted as an active nuclear factor of NF-κB dependent pathway in innate immunity of M. japonicus. | |||
TO cite this article:LIU Ning,SUN Jiejie,WANG Xianwei, et al. Characterization of Akirin in the innate immune response of the kuruma shrimp Marsupenseus japonicus[OL].[27 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4664578 |
2. Cloning, phylogeny and expression correlated to gonads development of Leptin genes in blunt snout bream | |||
ZHAO Honghao,ZENG Cong,YI Shaokui,WAN Shiming,Chen Baixiang,GAO Zexia | |||
Aquatic Science 14 November 2015 | |||
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Abstract:To investigate the leptin related genes expression patterns and their possible function during the gonadal development in fish, the cDNA and genomic sequences of leptin, leptin receptor (leptinR), and leptin receptor overlapping transcript like-1 (leprotl1) were cloned and their expression levels were quantified in the different gonadal development stages of Megalobrama amblycephala. The results showed that the full length cDNA sequences of leptin, leptinR, and leprotl1were 953, 3432 and 1676 bp, coding 168, 1,082, and 131 amino acid polypeptides, and the genomic sequences were 1836 bp, 28528 bp and 5480 bp, which respectively had 3, 15 and 4 exons, respectively. The phylogenetic analysis revealed that three genes were relatively conserved in fish species. Quantitative real-time PCR results showed that the three genes were ubiquitously expressed in all examined tissues during the different gonadal development stages. The leptin and leptinR took part in the onset of puberty, especially in female M. amblycephala, by increasing the expression levels in brain during the stage I to III of ovary. The expression levels of leptin and leptinR had significant differences between male and female in HPG axis tissues (P<0.05). The leptinR had the same variation tendency with leptin, but the opposite changes of expression levels were found in leprotl1, which may resist the expression of leptinR for inhibiting the function of leptin in target organ. These findings revealed details about the possible role of these genes in regulating gonadal maturation in fish species. | |||
TO cite this article:ZHAO Honghao,ZENG Cong,YI Shaokui, et al. Cloning, phylogeny and expression correlated to gonads development of Leptin genes in blunt snout bream[OL].[14 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4661884 |
3. Proteomic analysis revealed that cellular Stress Granule pathway is disrupted by Grass carp reovirus infection | |||
Xu Dan,Song Lang,Wang Hao,Xu Xiaoyan,Lu Liqun | |||
Aquatic Science 12 March 2015 | |||
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Abstract:Grass carp (Ctenopharyngodon idella) hemorrhagic disease, caused by grass carp reovirus (GCRV), is emerging as a serious problem in grass carp aquaculture. To better understand the molecular responses to GCRV infection, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization tandem mass spectroscopy were performed to investigate altered proteins in C. idella kidney (CIK) cells. Differentially expressed proteins in mock infected CIK cells and GCRV-infected CIK cells were compared. Twenty-three differentially expressed spots were successfully identified (22 upregulated spots and 1 downregulated spot), which included cytoskeleton proteins, macromolecular biosynthesis-associated proteins, stress response proteins, signal transduction proteins, energy metabolism-associated proteins and ubiquitin proteasome pathway-associated proteins. Moreover, 10 of the corresponding genes of the differentially expressed proteins were quantified by real-time reverse transcription polymerase chain reaction to examine their transcriptional profiles. The T cell internal antigen 1(TIA1) and Ras-GTPase-activating SH3-domain-binding protein1(G3BP1) proteins of the cellular Stress Granule pathway from grass carp Ctenopharyngodon idella (designated as CiTIA1 and CiG3BP1) were upregulated and downregulated, respectively, during GCRV infection. The full-length cDNA of CiTIA1 was 2753 bp with an open reading frame (ORF) of 1155bp that encodes a putative 385-amino acid protein. The full-length cDNA of CiG3BP1 was 2271bp ORF of 1455bp that encodes a putative 485-amino acid protein. Phylogenetic analysis revealed that the complete ORFs of CiTIA1 and CiG3BP1 were very similar to zebrafish and well characterized mammalian homologs. The expressions of the cellular proteins CiTIA1 and CiG3BP1 response to GCRV were validated by western blotting. This study provides useful information on the proteomic and cellular stress granule pathway's responses to GCRV infection, which adds to our understanding of viral pathogenesis. | |||
TO cite this article:Xu Dan,Song Lang,Wang Hao, et al. Proteomic analysis revealed that cellular Stress Granule pathway is disrupted by Grass carp reovirus infection[OL].[12 March 2015] http://en.paper.edu.cn/en_releasepaper/content/4633739 |
4. miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle | |||
Yan Biao,Wang Zhenhua,Zhao Jinliang | |||
Aquatic Science 14 October 2014 | |||
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Abstract:MyoD is one of the helix-loop-helix proteins regulating muscle-specific gene expression in tilapia. Tight regulation of the MyoD protein level is necessary for the precise regulation of skeletal muscle development. MicroRNAs (miRNAs) are a class of regulatory RNAs that post-transcriptionally regulate gene expression. An increasing amount of evidence has suggested that miRNAs play an important role in regulating skeletal muscle development. We reasoned that MyoD expression may be regulated by miRNAs. Predictions from bioinformatics have identified a putative miR-203b target site in the 3′-UTR of the MyoD gene. Interestingly, miR-203b expression is negatively correlated with MyoD expression, whereas miR-203b suppression leads to a significant increase in MyoD expression, thereby activating MyoD downstream genes. A 3′-UTR luciferase reporter assay further verifies the direct interaction between miR-203b and MyoD. Taken together, our results reveal a novel molecular mechanism inwhich miRNA participates in transcriptional circuits that regulate gene expression in tilapia skeletal muscle.????? | |||
TO cite this article:Yan Biao,Wang Zhenhua,Zhao Jinliang. miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle[OL].[14 October 2014] http://en.paper.edu.cn/en_releasepaper/content/4613624 |
5. Dynamic expression pattern of Corticotropin releasing hormone and Urotensin I, II gene and responses to acute salinity and temperature challenge in zebrafish during early development | |||
Lei Luo,Aqin Chen,Jianghong Xiong,Congcong Hu,Weiqun Lu | |||
Aquatic Science 26 December 2013 | |||
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Abstract:Corticotropin releasing hormone (CRH), urotensin I (UI) and urotensin II (UII) are found throughout vertebrate species from fish to human. To further understand the role of CRH, UI and UII in teleosts during development, we investigated the expression pattern of CRH, UI, UIIα and UIIβ, and the response to acute salinity and temperature challenge during the early development of zebra?sh, Danio rerio. The results reveal that CRH, UI, UIIα and UIIβ mRNA are detected from 0hpf, and the relative expression levels alter along with development from 0hpf to 6dpf and reach the highest level at 6dpf except UIIα at 5dpf. After zebrafish at 2, 3, 4 and 5 dpf were exposed to an acute stress for 15min, no significant difference in expression levels of CRH, UI, UIIα and UIIβ mRNA was observed in response to acute osmotic (30ppt) stress compared with control, while cold stress (18℃) and hot stress (38℃) generally inhibited the mRNA levels of CRH, UI, UIIα and UIIβ at each time point apart from at 3dpf. These indicate that the contribution of CRH, UI, UIIα and UIIβ to stress response in zebrafish may be stressor-specific during early development. Overall, results from this study offer new insights into the developmental and temperature-specific function of CRH, UI, UIIα and UIIβ in zebrafish embryos and larvae. | |||
TO cite this article:Lei Luo,Aqin Chen,Jianghong Xiong, et al. Dynamic expression pattern of Corticotropin releasing hormone and Urotensin I, II gene and responses to acute salinity and temperature challenge in zebrafish during early development[OL].[26 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4578577 |
6. Daily rhythms of urotensin I and II gene expression and hormone secretion in the caudal neurosecretory system of the euryhaline flounder (Platichthys flesus) | |||
Lv Weiqun ,Ying Zhang,Jianghong Xiong,Richard Balment | |||
Aquatic Science 04 April 2013 | |||
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Abstract:The caudal neurosecretory system (CNSS) is a unique neuroendocrine structure for environmental adaptation in fish, and is the major site of expression and secretion of urotensin I (UI) and II (UII). This study examined daily changes in mRNA expression and the secretion profile of UI and UII in the CNSS. Daily rhythms were observed in mRNA level of CNSS UI, urophysis UI, plasma UII, glucose, potassium and sodium. No statistically significant (Cosinor, P>0.05) diel rhythmicity in mRNA level of CNSS UII, urophysis UII, cortisol, lactate, osmolality and chloride were detected. The calculated acrophase of sodium, cortisol, plasma UII, urophysis UII, urophysis UI and mRNA level of CNSS UI rhythms were recorded at 13:04 h, 13:39 h, 14:45 h, 15:27 h. 14:41 h and 14:39 h, respectively and a positive relationship was evident among them. The acrophase of glucose and potassium rhythms were recorded at 18:57 h and 22:35 h, respectively. The glucose levels increased progressively at the onset of the UII surge at 15:00 h and reached peak values at dusk. The results support the hypothesis that the CNSS may play a role in the control of co-ordinated daily changes in energy mobilization, nutritional behavior and osmoregulatory systems in euryhaline flounder. Our findings described for the first time the existence of daily rhythms of CNSS hormone expression and secretion in Platichthys flesus. These results reveal the importance of taking into account the time of day when assessing stress responses and evaluating UI and UII as physiological indicators of stress in this species. | |||
TO cite this article:Lv Weiqun ,Ying Zhang,Jianghong Xiong, et al. Daily rhythms of urotensin I and II gene expression and hormone secretion in the caudal neurosecretory system of the euryhaline flounder (Platichthys flesus)[OL].[ 4 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4532623 |
7. Biotransformation of selenium by seaweed Gracilaria lemaneiformis and its effects on the antioxidant enzymes | |||
ZuoYa,Yang Yufeng,Chen Tianfeng,Zheng Wenjie | |||
Aquatic Science 02 April 2013 | |||
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Abstract:The accumulation and biotransformation of selenium (Se) in seaweed Gracilaria lemaneiformis, collected from Nan-ao Island, Shantou, China were investigated in the present study. The seaweed was cultured for 12 days in medium containing 0-2000 mg/L of selenite, and the results showed that, under the concentrations lower than 750 mg/L, Se promoted the growth of seaweed, and increased the contents of chlorophyll α, carotenoids, and phycobiliprotein. When the concentrations of Se was higher than 1000 mg/L, the photosynthetic pigments in the seaweed were significantly lower than that of control, while the maximum photochemical efficiency (Fv/Fm) was also decreased. The accumulation of Se in the biomass was linearly correlated to the dose of Se added into the media. Moreover, the organic rate of Se in seaweed was over 80% in all the groups. Furthermore, Se enrichment induced overproduction of reactive oxygen species (ROS) and membrane lipid peroxidation (LPO) in the seaweed cells, as accompanied by decrease in the activities of antioxidant enzymes, such as catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD). Taken together, these results suggest that G. lemaneiformis possesses tolerance to Se and could prevent phytotoxicity by altering various metabolic processes. In addition, G. lemaneiformis exhibits great potential to transform inorganic Se into organic Se and therefore could be a sound candidate for the production of Se-enriched functional food. | |||
TO cite this article:ZuoYa,Yang Yufeng,Chen Tianfeng, et al. Biotransformation of selenium by seaweed Gracilaria lemaneiformis and its effects on the antioxidant enzymes[OL].[ 2 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4535536 |
8. Comparative analysis of nuclear oligomerization domain proteins (NOD)-1 and NOD2 in orange-spotted grouper (Epinephelus coioides) | |||
HOU Qinghua,LU Danqi,YI Shibai,DING Xu,SUN Yan,ZHANG Huixian,LIU Xiaochun,ZHANG Yong,LIN Haoran | |||
Aquatic Science 25 December 2012 | |||
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Abstract:Nucleotide-binding oligomerization domain-containing proteins-1 and-2 (NOD1 and NOD2) are members of the NOD-like receptors (NLRs) family. They are both cytoplasmic receptors, and sense microbial infections/danger molecules to induce host innate immune response. In this study, the full-length ORF sequences of NOD1 and NOD2 were cloned, and the putative amino acid sequences were identi?ed in orange-spotted grouper (Epinephelus coioides). The complete open reading frame (ORF) of grouper NOD1 contained 2823 bp encoding a 940 amino acid protein. Grouper NOD2 cDNA contained a 2967 bp ORF, encoding a protein of 988 amino acid residues. Both grouper NOD1 and NOD2 had similar domains to human and fish counterparts. Phylogenetic tree analysis showed that grouper NOD1 clustered with grass carp, zebra?sh and channel cat?sh, while NOD2 was most closely related to fugu. Expression patterns of grouper NOD1 and NOD2 were next studied. NOD1 had the highest level of expression in skin while NOD2 in trunk kidney. Post Vibrio alginolyticus (strain EcGS020401), lipopolysaccharide (LPS) or PolyI:C challenges, gene expression of grouper NOD1 and NOD2 was stimulated to different extents. NOD1 showed a significant enhancement after LPS stimulation, but NOD2 increased more significantly after PolyI:C invasion, indicating that NOD1 and NOD2 may exert different effects on the eradication of bacteria and virus. The adaptor protein RIP-like-interacting CLARP kinase (RICK) and downstream molecule interleukin-8 (IL-8) were also induced at different levels after stimulation, which indicated that NOD1 and NOD2 signal transduction was involved in grouper innate immune protection against bacterial and viral infections. | |||
TO cite this article:HOU Qinghua,LU Danqi,YI Shibai, et al. Comparative analysis of nuclear oligomerization domain proteins (NOD)-1 and NOD2 in orange-spotted grouper (Epinephelus coioides)[OL].[25 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4499074 |
9. RA mediates meiosis onset in germ cells of Nile tilapia, Oreochromis niloticus | |||
DONG Ranran,JIANG Wentao,YANG Shijie,FANG Lingling,FENG Ruijuan,ZHOU Linyan,WANG Deshou | |||
Aquatic Science 29 November 2012 | |||
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Abstract:Recent studies have implicated that retinoic acid (RA) signal pathway played a critical role in meiosis initiation in mammals, birds and amphibians. However, the role of RA in meiosis initiation of teleosts remains to be elucidated. In the present study, RA synthetase (aldh1a2) and catabolic enzymes (cyp26a1 and cyp26b1) were cloned from the Nile tilapia. Tissue distribution analysis showed that aldh1a2, cyp26a1 and cyp26b1 were expressed both in gonadal and non-gonadal tissues (heart, liver, spleen, intestine, kidney, muscle, brain, gill, testis and ovary). Expression of aldh1d2 and cyp26a1 showed significant sexual dimorphism in gonads, while cyp26b1 showed no difference between ovary and testis. Moreover, ontogeny study revealed that aldh1a2 was up-regulated just before meiosis initiation of germ cells in XX and XY gonads. Expression level of cyp26a1 was very low and showed no significant difference between XX and XY gonads at 20 and 30 dah (day after hatching). Nevertheless, it was consistently low in XY gonads while up-regulated in XX gonads from 90 to 180 dah. Expression of cyp26b1 was very low in both XX and XY gonads at all sampling points except for 30 dah when it was significantly up-regulated in XY gonads. Treatment of XX fish by citral (RA synthetase inhibitor) alone or together with RA were performed from 5 to 70 dah. Citral treatement resulted in delayed meiosis initiation and partial or complete sex reversal. On the contrary, RA rescued citral treated fish and induced normal meiosis initiation and oogenesis. Immunohistochemistry analysis in the ovotestis showed that aromatase signals were detected in interstitial cells in ovarian tissues while Dmrt1 signals were confined to Sertoli cells in testicular tissues and around degenerating oocytes. Furthermore, aldh1a2 and meiosis marker scp3 were up-regulated in premeiotic phase of germ cells in sex reversed gonads. In contrast, cyp26a1 was down-regulated while cyp26b1 remained relatively stable throughout the examined time points in citral treated fish. These results indicated that RA is indispensable for meiosis initiation, and probably Cyp26a1, instead of Cyp26b1, was involved in meiosis initiation of germ cells in fish. In conclusion, the role for RA in meiosis initiation may be conserved in vertebrates. | |||
TO cite this article:DONG Ranran,JIANG Wentao,YANG Shijie, et al. RA mediates meiosis onset in germ cells of Nile tilapia, Oreochromis niloticus[OL].[29 November 2012] http://en.paper.edu.cn/en_releasepaper/content/4499107 |
10. Application of whole-implicit algorithm and virtual neural lattice in pelagic longline modeling | |||
ZHANG Xinfeng,CAO Daomei,SONG Liming | |||
Aquatic Science 22 March 2012 | |||
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Abstract:This study developed a whole-implicit algorithm and virtual neural lattice to model and simulate the dynamics of pelagic longline gear. The programming, model calculation and behavior simulation of longline dynamics were implemented in R language. Equilibrium state and stable solution of the models were reached. The hooks were more stable in the deepest water layer than that in the midwater layer. It indicated that the whole-implicit method and virtual neural lattice were high efficient and accurate in solving the complicated engineering equations of longline gear, also in the presentation of the motion of longline in sea water. | |||
TO cite this article:ZHANG Xinfeng,CAO Daomei,SONG Liming. Application of whole-implicit algorithm and virtual neural lattice in pelagic longline modeling[OL].[22 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4472683 |
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