Select Subject

All (8830)

Agronomy (158)

Animal Husbandry,... (64)

Aquatic Science (16)

Astronomy (36)

Aviation (22)

Basic Medicine (184)

Biology (504)

Chemical Engineering (103)

Chemistry (635)

Civil and Archite... (105)

Clinical Medicine (236)

Computer Science ... (924)

Dynamic and Elect... (121)

Earth Science (119)

Economics (67)

Education (31)

Electrics, Commun... (927)

Energy Science an... (67)

Engineering and T... (15)

Environmental Sci... (140)

Food Science and ... (63)

Forestry (36)

Hydraulic Enginee... (40)

Mechanics (240)

Metallurgical Eng... (32)

Mine Engineering ... (38)

Nuclear Science a... (21)

Pharmacy (98)

Physics (920)

Preventive Medici... (37)

Psychology (20)

Security Science ... (17)

Sports Science (6)

Surveying and Map... (15)

Textile Science a... (16)

Traditional Chine... (56)

Transportation En... (57)

Select/Unselect all

For Selected Papers

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list


	1. Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-Feng
	ZHAO Xiaona
	Animal Husbandry, Veterinary Medicine 05 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Yu-Ping-Feng is a well-known traditional Chinese multi herbal formula, and it is frequently being used to treat cold and flu and inflammation-associated diseases. We aim to evaluate the immunostimulatory activity of polysaccharide from Yu-Ping-Feng(YPF-PS) in vitro on chicken macrophage to make clear the bioactive molecular of Yu-Ping-Feng. Gel permeation chromatography (GPC) and gas chromatography?mass spectrometry (GC-MS) analysis, amino acid analysis and nuclear magnetic resonance (NRM) spectroscopy elucidated the structure of the YPF-PS. In order to evaluate the biological activities of YPF-PS, lipopolysaccharides (LPS)-induced cell proliferation and phagocytosis rate and the levels of cytokine: nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin-1 beta, interleukin-6 (IL-1β, IL-6), interferon beta (IFN-β) and costimulatory molecules CD80, CD86 in chicken macrophage were detected. The results indicated that molecular weight the weight of YPF-PS was distributed on 5 KD~500 KDa, which is a mixture of glucose (Glu), fructose (Fru), arabinose (Ara) and few rhamnose with the molar ratio of 61.29: 55.47: 99.67: 19.58, having anomeric carbon signals of β-L-arabino furanoside, α-L-rhamnoside, α-D-glucopyranoside and β-D-fructofuranose. Furthermore, cell immunology experiments show that YPF-PS at dosage of 15.62 μgmL -1 could increase the LPS-induced cell proliferation and phagocytosis rate significantly in chicken macrophage, moreover, the levels of cytokine: NO, TNF-α, IL-1β, IL-6 and IFN-βin cells elevated obviously, equally shocking, costimulatory molecules CD80, CD86 were detected having a tremendous enhancement. We believe that the YPF-PS has potent immunomodulatory properties and could be explored as a novel potential immunostimulant for the food and pharmaceutical purpose.
	TO cite this article:ZHAO Xiaona. Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-Feng[OL].[ 5 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4686482


	2. Effects of polysaccharides from Platycodon Grandiflorum on immunity-enhancing activity in vitro
	ZHAO Xiaona
	Animal Husbandry, Veterinary Medicine 05 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:This study evaluated the immunity-enhancing activity of polysaccharides from Platycodon Grandiflorum (PGPS) in vitro. Lymphocyte proliferation, cell cycle distribution, and percentages of CD4+ and CD8+ T cells were investigated. Different concentrations of PGPS (PGPStc, PGPS60c, PGPS80c and PGPStp) were added to peripheral blood T lymphocytes. Results showed that PGPStc markedly enhanced T lymphocyte proliferation individually or synergistically with phytohemagglutinin (PHA) at most concentrations and the lymphocyte proliferation rates were the highest. Then, the active sites of PGPStc and PGPS60c were subsequently selected. Lymphocyte cell cycle distribution and percentages of CD4+ and CD8+ T cells were determined by flow cytometry. At most time points, PGPStc could promote lymphocytes enter into S and G2/M phases, while increasing the percentages of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments.
	TO cite this article:ZHAO Xiaona. Effects of polysaccharides from Platycodon Grandiflorum on immunity-enhancing activity in vitro[OL].[ 5 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4686485

Select/Unselect all

For Selected Papers

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Rank

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item