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1. Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-Feng | |||
ZHAO Xiaona | |||
Animal Husbandry, Veterinary Medicine 05 May 2016 | |||
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Abstract:Yu-Ping-Feng is a well-known traditional Chinese multi herbal formula, and it is frequently being used to treat cold and flu and inflammation-associated diseases. We aim to evaluate the immunostimulatory activity of polysaccharide from Yu-Ping-Feng(YPF-PS) in vitro on chicken macrophage to make clear the bioactive molecular of Yu-Ping-Feng. Gel permeation chromatography (GPC) and gas chromatography?mass spectrometry (GC-MS) analysis, amino acid analysis and nuclear magnetic resonance (NRM) spectroscopy elucidated the structure of the YPF-PS. In order to evaluate the biological activities of YPF-PS, lipopolysaccharides (LPS)-induced cell proliferation and phagocytosis rate and the levels of cytokine: nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin-1 beta, interleukin-6 (IL-1β, IL-6), interferon beta (IFN-β) and costimulatory molecules CD80, CD86 in chicken macrophage were detected. The results indicated that molecular weight the weight of YPF-PS was distributed on 5 KD~500 KDa, which is a mixture of glucose (Glu), fructose (Fru), arabinose (Ara) and few rhamnose with the molar ratio of 61.29: 55.47: 99.67: 19.58, having anomeric carbon signals of β-L-arabino furanoside, α-L-rhamnoside, α-D-glucopyranoside and β-D-fructofuranose. Furthermore, cell immunology experiments show that YPF-PS at dosage of 15.62 μgmL -1 could increase the LPS-induced cell proliferation and phagocytosis rate significantly in chicken macrophage, moreover, the levels of cytokine: NO, TNF-α, IL-1β, IL-6 and IFN-βin cells elevated obviously, equally shocking, costimulatory molecules CD80, CD86 were detected having a tremendous enhancement. We believe that the YPF-PS has potent immunomodulatory properties and could be explored as a novel potential immunostimulant for the food and pharmaceutical purpose. | |||
TO cite this article:ZHAO Xiaona. Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-Feng[OL].[ 5 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4686482 |
2. Effects of polysaccharides from Platycodon Grandiflorum on immunity-enhancing activity in vitro | |||
ZHAO Xiaona | |||
Animal Husbandry, Veterinary Medicine 05 May 2016 | |||
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Abstract:This study evaluated the immunity-enhancing activity of polysaccharides from Platycodon Grandiflorum (PGPS) in vitro. Lymphocyte proliferation, cell cycle distribution, and percentages of CD4+ and CD8+ T cells were investigated. Different concentrations of PGPS (PGPStc, PGPS60c, PGPS80c and PGPStp) were added to peripheral blood T lymphocytes. Results showed that PGPStc markedly enhanced T lymphocyte proliferation individually or synergistically with phytohemagglutinin (PHA) at most concentrations and the lymphocyte proliferation rates were the highest. Then, the active sites of PGPStc and PGPS60c were subsequently selected. Lymphocyte cell cycle distribution and percentages of CD4+ and CD8+ T cells were determined by flow cytometry. At most time points, PGPStc could promote lymphocytes enter into S and G2/M phases, while increasing the percentages of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments. | |||
TO cite this article:ZHAO Xiaona. Effects of polysaccharides from Platycodon Grandiflorum on immunity-enhancing activity in vitro[OL].[ 5 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4686485 |
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